Metabolic flux analysis of CHO cells at growth and non-growth phases using isotopic tracers and mass spectrometry

Metabolic Engineering

Volumn 13, Issue 5, 2011, Pages 598-609

  Ahn, Woo Suk ^a   Antoniewicz, Maciek R ^a  

^a University of Delaware   (United States)

Author keywords

Mammalian cell culture; Mass spectrometry; Metabolic flux map; Non stationary flux analysis; Stable isotope tracers

Indexed keywords

BIOPHARMACEUTICAL INDUSTRY; BIOTHERAPEUTICS; CELL LINES; CHINESE HAMSTER OVARY CELLS; CHO CELL; ENERGY METABOLISM; EXPONENTIAL PHASE; EXTRACELLULAR; FED-BATCH CULTURES; FLUX ANALYSIS; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GROWTH PHASE; HIGH FLUX; INTRACELLULAR METABOLITES; ISOTOPIC TRACER; KETOGLUTARATE; MALIC ENZYMES; MAMMALIAN CELL CULTURE; MEDIUM OPTIMIZATION; METABOLIC FLUX; METABOLIC FLUX ANALYSIS; NONSTATIONARY; OXIDATIVE PENTOSE PHOSPHATE PATHWAYS; PYRUVATES; RECOMBINANT PROTEIN; REDOX METABOLISM; STABLE ISOTOPE TRACERS; STATIONARY PHASE; TCA CYCLE;

ANIMAL CELL CULTURE; BATCH CELL CULTURE; CELLS; CYTOLOGY; ENZYME ACTIVITY; GAS CHROMATOGRAPHY; GLUCOSE; ISOTOPES; MAMMALS; MASS SPECTROMETRY; METABOLITES; PATHOLOGY; PHYSIOLOGY; PROTEINS;

METABOLISM;

2 OXOGLUTARIC ACID; GLUCOSE; GLUTAMIC ACID; LACTIC ACID; MALATE DEHYDROGENASE (DECARBOXYLATING); OXALOACETIC ACID; PENTOSE PHOSPHATE; PYRUVIC ACID; TRACER;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL GROWTH; CELL METABOLISM; CHO CELL; CONTROLLED STUDY; DYNAMICS; ENERGY METABOLISM; GLYCOLYSIS; GROWTH CURVE; ISOTOPE TRACING; MASS FRAGMENTOGRAPHY; MASS SPECTROMETRY; METABOLITE; NONHUMAN; PENTOSE PHOSPHATE CYCLE; PRIORITY JOURNAL;

ANIMALS; CARBON ISOTOPES; CELL PROLIFERATION; CHO CELLS; CITRIC ACID CYCLE; CRICETINAE; CRICETULUS; GLUCOSE; GLYCOLYSIS; MASS SPECTROMETRY;

CRICETULUS GRISEUS; MAMMALIA;

EID: 80052032471     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2011.07.002     Document Type: Article

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