Mg2+ binds to the surface of thymidylate synthase and affects hydride transfer at the interior active site

Journal of the American Chemical Society

Volumn 135, Issue 20, 2013, Pages 7583-7592

  Wang, Zhen ^a,d   Sapienza, Paul J ^b   Abeysinghe, Thelma ^a   Luzum, Calvin ^a,e   Lee, Andrew L ^b   Finer Moore, Janet S ^c   Stroud, Robert M ^c   Kohen, Amnon ^a  

^a E11 SSH   (United States)

^b University of North Carolina   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^e Space and Naval Warfare Systems Center Pacific   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENTROPY OF ACTIVATION; INTRINSIC KINETICS; LONG RANGE INTERACTIONS; NMR RELAXATION EXPERIMENTS; NON-CONSERVED RESIDUES; STRUCTURAL EVIDENCE; TEMPERATURE DEPENDENCE; THYMIDYLATE SYNTHASE;

ANTIBIOTICS; ENZYME ACTIVITY; ESCHERICHIA COLI; ISOTOPES; KINETICS; PROTEINS;

HYDRIDES;

ANTIBIOTIC AGENT; BACTERIAL ENZYME; FOLIC ACID; GLUTAMIC ACID; ISOTOPE; MAGNESIUM ION; THYMIDYLATE SYNTHASE; MAGNESIUM; WATER;

ARTICLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENTHALPY; ENTROPY; ENZYME ACTIVE SITE; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME STABILITY; ENZYME STRUCTURE; ESCHERICHIA COLI; FLUORESCENCE ANALYSIS; METAL BINDING; TEMPERATURE DEPENDENCE; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; METABOLISM; SURFACE PROPERTY;

BINDING SITES; ESCHERICHIA COLI; MAGNESIUM; MODELS, MOLECULAR; MOLECULAR STRUCTURE; SURFACE PROPERTIES; THYMIDYLATE SYNTHASE; WATER;

EID: 84878237195     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja400761x     Document Type: Article

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