Enzyme dynamics and hydrogen tunnelling in a thermophilic alcohol dehydrogenase

Nature

Volumn 399, Issue 6735, 1999, Pages 496-499

  Kohen, Amnon ^a   Cannio, Raffaele ^b   Bartoluccl, Simonetta ^c   Klinman, Judith P ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b CNR   (Italy)

^c UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL DEHYDROGENASE; HYDROGEN;

ARTICLE; CATALYST; ENZYME MECHANISM; ENZYME SUBSTRATE; GEOBACILLUS STEAROTHERMOPHILUS; NONHUMAN; PRIORITY JOURNAL; PROTON TRANSPORT; THERMODYNAMICS; THERMOPHILIC BACTERIUM;

ALCOHOL DEHYDROGENASE; BACILLUS STEAROTHERMOPHILUS; BENZYL ALCOHOL; CATALYSIS; HYDROGEN; MODELS, CHEMICAL; NAD; TEMPERATURE;

BACTERIA (MICROORGANISMS); GEOBACILLUS STEAROTHERMOPHILUS;

EID: 0033519723     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/20981     Document Type: Article

References (28)

1. Kohen, A. & Klinman, P. J. Enzyme catalysis beyond chemical paradigms. Acc. Chem. Res. 31, 387-404 (1988).
2. Bell, R. P. The Tunneling Effect in Chemistry (Chapman & Hall, London, 1989).
3. Steinfeld, J. I., Fran , J. S. & H , W. L. (eds) Chemical Kinetics and Dynamics (Prentice Hall, Upper Saddle River, NJ, 1998).
4. Banderskii, V. A., Makarov, D. E. & Wight, C. A. Adv. Chem. Phys. 88, 151-207 (1994).
5. Cho, Y., Murray, C. J. & Klinman, J. P. Hydrogen tunneling in enzyme reactions. Science 243, 1323-13 (1 ).
6. Brooks, C. L. I., Karplus, M. & Pettitt, B. M. Adv. Chem. Phys. 71, 14-23; 33-59; 75-233; (1998).
7. Rasmussen, B. F., Stack, A. M., Bingo, D. & Petiko, G. A. Crystalline A fraction below the dynamical transition at 220-K. Nature 357, 423-424 (1992).
8. Rudd, P. M. et al. modify the dynamic stability and functional activity of an enzyme. Biochemistry, 33, 17-22 (1994).
9. Kohen, A., Jonsson, T. & Klinman, P. J. of protein glycosylation on catalysis: changes in hydrogen tunneling and enthalpy of activation in the glucose oxidase reaction. Biochemistry 36, 2611 (1997).
10. Cannio, R., Rossi M. & Bartolucci, S. A amino acid substitutions are for the higher the stability of a novel NAD(+)-dependent bacillus alcohol dehydrogenase. Eur. J. Biochem. 22, 345-352 (1994).
11. Guagliardi, A. et al. Purification and ch tion of the alcohol dehydrogenase of bacillus growing at 70°C. Int. J. Biochem. Cell Biol. 28, 239-246 (1 ).
12. , S., H , G. & P. Conformational stability of lactate dehydrogenase from Bacillus th . Biochem. Soc. Trans. 6, 1197 (1978).
13. y, P., K , J., S gor, A. & G. A. Adjustment of conformational stability is a key in the thermal tion of protein. Proc. Natl. Acad. Sci. USA ( ).
14. V , P. G. & P. R. H. stability, dynamics and enzyme activity in 3-phosphoglycerate kin from past and thermophilus. J. Mol. Biol.320, ( ).
15. Wrba, A., Schweiger, A., S , V., , R. & Zavodsky, P. D-gly -3-ph dehydrogenase from the The . Biochemistry 29, 7584-7592 ( ).
16. Lin, S. & S , W. H. Tunneling in ination reactions - structural effects on the secondary beta-isotope effects. J. Am. Chem. Soc. 116, 6107-6110 (1994).
17. S ders, W.H. Calculations of isotope effects in ting reactions. New experimental crite for tunneling in . J. Am. Chem. Soc. 107, 164-169 (1985).
18. J. & study of tunneling and coupled motion. J. Am. Chem. Soc. 121, 1997-( ).
19. L. & , W. H. of Isotopic Molecules (Krieger, Mal , FL, 1987).
20. , M. of protein flexibility to thermostability. Protein Eng. 1, 477-480 ( ).
21. Vol' , M. V., , R. R., M , A. K., Urashadze, Z. D. & Khar , Y. I. The theory of enzyme catalysis. Mol. Biol. 6, 347-353 (1972).
22. , J. K. & Wa , A. On the relationship between the dispersed proton transfer and the role of oscillations. Proc. . Natl Acad. Sci. USA 94, 12360-12365 (1997).
23. D., & , S. D. Large kinetic isotope effect in enzymatic proton transfer and the role of substrate oscillations. Proc. Natl Acad. Sci. USA 94, 12360-12365 (1997).
24. Burgis, D. & Hynes, J. T. in the Enzyme Catalysis Process (eds Cooper, A., Houben, J. & Chien, L.) 293-303 (Plenum, New York, 1989).
25. W. J. & W. Vibrationally enhanced tunneling as a mechanism for enzymatic hydrogen transfer. Biophys. J. 63, (1992).
26. D. & Hynes, J. T. Dynamical theory of proton tunneling transfer rates in solution - general formulation. Chem. Phys. 174 335-( ).
27. D. & S. D. chemistry in the presence of a strongly mmetically coupled . J. Chem. Phys. 108, 3620-3625 (1998).
28. G. N. & J. F. Inefficient lactate dehydrogenases of fishes. Nature , (1979).