Exploring the molecular linkage of protein stability traits for enzyme optimization by iterative truncation and evolution

Biochemistry

Volumn 51, Issue 24, 2012, Pages 4850-4867

  Speck, Janina ^a,b   Hecky, Jochen ^b,c   Tam, Heng Keat ^b   Arndt, Katja M ^a,b   Einsle, Oliver ^b   Müller, Kristian M ^a,b  

^a UNIVERSITY OF POTSDAM   (Germany)

^b UNIVERSITY OF FREIBURG   (Germany)

^c Cellgenix GmbH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA SHUFFLING; ELEVATED TEMPERATURE; EXTERNAL STRESS; FULL-LENGTH ENZYMES; GUANIDINIUM; IN-VIVO; INDUSTRIAL USE; LACTAMASES; MOLECULAR LINKAGES; N-TERMINALS; PHYSICAL STABILITY; PROTEIN ENGINEERING; PROTEIN STABILITY; PROTEOLYTIC RESISTANCE; STRUCTURAL STRESS; WILD TYPES; WILD-TYPE PROTEINS;

ENZYMES; GENETIC ENGINEERING; INDUSTRIAL APPLICATIONS; PROTEINS; STABILITY;

CHEMICAL STABILITY;

BETA LACTAMASE TEM 1; GUANIDINE; MUTANT PROTEIN; PROTEINASE;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CRYSTAL STRUCTURE; DNA SHUFFLING; HEAT; IN VIVO STUDY; MUTATION; NONHUMAN; PEPTIDE LIBRARY; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN STABILITY; WILD TYPE;

BETA-LACTAMASES; DIRECTED MOLECULAR EVOLUTION; ENZYME STABILITY; GENE LIBRARY; GUANIDINE; MODELS, MOLECULAR; PROTEIN CONFORMATION; PROTEIN UNFOLDING; PROTEOLYSIS; SEQUENCE DELETION; TEMPERATURE; THERMODYNAMICS;

EID: 84862528500     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi2018738     Document Type: Article

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