Rapid in vivo evolution of a β-lactamase using phagemids

Biotechnology and Bioengineering

Volumn 68, Issue 1, 2000, Pages 121-125

  Long McGie, Jeffrey ^a,b   Liu, Amy D ^a   Schellenberger, Volker ^a  

^a GENENCOR INTERNATIONAL INC   (United States)

^b WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

lactamase; Antibiotic resistance; Directed evolution; Phagemid

Indexed keywords

ANTIBIOTIC AGENT; BETA LACTAMASE; CEFOTAXIME; KANAMYCIN;

ANTIBIOTIC RESISTANCE; ARTICLE; CATALYST; ESCHERICHIA COLI; EVOLUTION; GENE MUTATION; MUTAGENESIS; NONHUMAN; PHAGEMID;

BACTERIOPHAGES; BETA-LACTAM RESISTANCE; BETA-LACTAMASES; CLONING, MOLECULAR; EVOLUTION, MOLECULAR; MOLECULAR BIOLOGY; POINT MUTATION;

ESCHERICHIA COLI; RNA VIRUSES;

EID: 0034607420     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(20000405)68:1<121::AID-BIT15>3.0.CO;2-X     Document Type: Article

References (16)

1. Arnold FH. 1998. Design by directed evolution. Ace Chem Res 31: 125-131.
2. Bradford PA, Sanders C. 1995. Development of test panel of β-lactamases expressed in a common Escherichia coli host background for evaluation of new β-lactam antibiotics. Antimicrob Agents Chemother 39: 308-3132.
3. Degnen GE, Cox EC. 1974. Conditional mutator gene in Escherichia coli: Isolation, mapping, and effector studies. J Bacteriol 117:477-487.
4. Domingo E, Escarmis C, Sevilla N, Moya A, Elena SF, Quer J, Novella IS, Holland JJ. 1996. Basic concepts in RNA virus evolution. FASEB J 10:859-864.
5. Huang W, Petrosino J, Hirsch M, Shenkin PS, Palzkill T. 1996. Amino acid sequence determinants of β-lactamase structure and activity. J Mol Biol 258:688-703.
6. Liao H, McKenzie T, Hageman R. 1986. Isolation of a thermostable enzyme variant by cloning and selection in a thermophile. Proc Natl Acad Sci USA 83:576-580.
7. Lipsitch M, Levin BR. 1997. The population dynamics of antimicrobial chemotherapy. Antimicrob Agents Chemother 41:363-373.
8. Low NM, Holliger P, Winter G. 1996. Mimicking somatic hypermutation: Affinity maturation of antibodies diplayed on bacteriophage using a bacterial mutator strain. J Mol Biol 260:359-368.
9. Miller JH. 1992. A short course in bacterial genetics. Plainview, NY: Cold Spring Harbor Laboratory Press.
10. Paul GC, Gerbaud G, Bure A, Philippon AM, Pangon B, Courvalin P. 1989. TEM-4, a new plasmid-mediated β-lactamase that hydrolyzes broad-spectrum cephalosporins in a clinical isolate of Escherichia coli. Antimicrob Agents Chemother 33:1958-1963.
11. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning: A laboratory manual. Plainview, NY: Cold Spring Harbor Laboratory Press.
12. Sirot D. 1995. Extended-spectrum plasmid-mediated β-lactamases. J Antimicrob Chem 36:19-34.
13. Sniegowski PD, Gerrish PJ, Lenski RE. 1997. Evolution of high mutation rates in experimental populations of E. coli. Nature 387:703-705.
14. Stemmer WPC. 1994. Rapid evolution of a protein in vitro by DNA shuffling. Nature 370:389-391.
15. Taddei F, Radman M, Maynard-Smith J, Toupance B, Gouyon PH, Godelle B. 1997. Role of mutator alleles in adaptive evolution. Nature 387: 700-702.
16. Zaccolo M, Gherardi E. 1999. The effect of high-frequency random mutagenesis on in vitro protein evolution: A study on TEM-1 β-lactamase. J Mol Biol 285:775-783.