Mutual stabilization of V(L) and V(H) in single-chain antibody fragments, investigated with mutants engineered for stability

Biochemistry

Volumn 37, Issue 38, 1998, Pages 13120-13127

  Wörn, Arne ^a   Plückthun, Andreas ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; IMMUNOGLOBULIN FRAGMENT; IMMUNOGLOBULIN FV; RECOMBINANT PROTEIN; THERMOLYSIN;

ARTICLE; IMMUNOGLOBULIN VARIABLE REGION; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN STABILITY; BIOSYNTHESIS; CHEMISTRY; GENETICS; HYDROLYSIS; ISOLATION AND PURIFICATION; METABOLISM; PROTEIN DENATURATION; PROTEIN ENGINEERING; SITE DIRECTED MUTAGENESIS;

HYDROLYSIS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN LIGHT CHAINS; IMMUNOGLOBULIN VARIABLE REGION; MUTAGENESIS, SITE-DIRECTED; PROTEIN DENATURATION; PROTEIN ENGINEERING; PROTEIN FOLDING; RECOMBINANT PROTEINS; THERMOLYSIN;

EID: 0032558362     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi980712q     Document Type: Article

References (45)

1. Wetlaufer, D. B. (1981) Adv. Protein Chem. 34, 61-92.
2. Creighton, T. E. (1978) Prog. Biophys. Mol. Biol. 33, 231-297.
3. Jaenicke, R. (1987) Prog. Biophys. Mol. Biol. 49, 117-237.
4. Anthony, J., Near, R., Wong, S. L., Iida, E., Ernst, E., Wettekind, M., Haber, E., and Ng, S. C. (1992) Mol. Immunol. 29, 1237-1247.
5. Stevens, P. W., Raffen, R., Hanson, D. K., Deng, Y. L., Berrios-Hammond, M., Westholm, F. A., Murphy, C., Eulitz, M., Wetzel, R., Solomon, A., Schiffer, M., and Stevens, F. J. (1995) Protein Sci. 4, 421-432.
6. Schormann, N., Murrell, J. R., Liepnieks, J. J., and Benson, M. D. (1995) Proc. Natl. Acad. Sci. U.S.A. 92, 9490-9494.
7. Frisch, C., Kolmar, H., and Fritz, H. J. (1994) Biol. Chem. Hoppe-Seyler 375, 353-356.
8. Hurle, M. R., Helms, L. R., Li, L., Chan, W., and Wetzel, R. (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 5446-5450.
9. Steipe, B., Schiller, B., Plückthun, A., and Steinbacher, S. (1994) J. Mol. Biol. 240, 188-192.
10. Stevens, F. J., Solomon, A., and Schiffer, M. (1991) Biochemistry 30, 6803-6805.
11. Ward, E. S., Güssow, D., Griffiths, A. D., Jones, P. T., and Winter, G. (1989) Nature 341, 544-546.
12. Davies, J., and Riechmann, L. (1994) FEBS Lett. 339, 285-290.
13. Hamers-Castermann, C., Atarhouch, T., Muyldermans, S., Robinson, G., Hamers, C., Songa, E. B., Bendahman, N., and Hamers, R. (1993) Nature 363, 446-448.
14. Muyldermans, S., Atarhouch, T., Saldanha, J., Barbosa, J. A. R. G., and Hamers, R. (1994) Protein Eng. 7, 1129-1135.
15. Desmyter, A., Transue, T. R., Ghahroudi, M. A., Thi, M. H., Poortmans, F., Hamers, R., Muyldermans, S., and Wyns, L. (1996) Nature Struct. Biol. 3, 803-811.
16. Spinelli, S., Frenken, L., Bourgeois, D., de Ron, L., Bos, W., Verrips, T., Anguille, C., Cambillau, C., and Tegoni, M. (1996) Nature Struct. Biol. 3, 752-757.
17. Bird, R. E., Hardman, K. D., Jacobson, J. W., Johnson, S., Kaufman, B. M., Lee, S. M., Lee, T., Pope, S. H., Riordan, G. S., and Whitlow, M. (1988) Science 242, 423-426.
18. Huston, J. S., Levinson, D., Mudgett-Hunter, M., Tai, M., Novotny, J., Margolies, M. N., Ridge, R. J., Bruccoleri, R. E., Haber, E., Crea, R., and Oppermann, H. (1988) Proc. Natl. Acad. Sci. U.S.A. 85, 5879-5883.
19. Proba, K., Honegger, A., and Plückthun, A. (1997) J. Mol. Biol. 265, 161-172.
20. Rudikoff, S., and Pumphrey, J. G. (1986) Proc. Natl. Acad. Sci. U.S.A. 83, 7875-7878.
21. Auffray, C., Sikorav, J. L., Ollo, R., and Rougeon, F. (1981) Ann. Immunol. (Inst. Pasteur) 132D, 77-88.
22. Heinrikson, R. L. (1977) Methods Enzymol. 47, 175-189.
23. Kabat, E. A., Wu, T. T., Perry, H. M., Gottesman, K. S., and Foeller, C. (1991) NIH Publication No. 91-3242, National Institutes of Health, Bethesda, MD.
24. Stemmer, W. P. C. (1994) Nature 370, 389-391.
25. Proba, K., Wörn, A., Honegger, A., and Plückthun, A. (1998) J. Mol. Biol. 275, 245-253.
26. Freund, C., Ross, A., Guth, B., Plückthun, A., and Holak, T. A. (1993) FEBS Lett. 320, 97-100.
27. Ge, L., Knappik, A., Pack, P., Freund, C., and Plückthun, A. (1995) in Antibody Engineering (Borrebaeck, C. A. K., Ed.) 2nd ed., pp 229-266, Oxford University Press, New York.
28. Studier, F. W., and Moffatt, B. A. (1986) J. Mol Biol. 189, 113-130.
29. Knappik, A., Krebber, C., and Plückthun, A. (1993) Biotechnology 11, 77-83.
30. Knappik, A., and Plückthun, A. (1994) Biotechniques 17, 754-761.
31. Tang, Y., Jiang, N., Parakh, C., and Hilvert, D. (1996) J. Biol. Chem. 271, 15682-15686.
32. Plückthun, A., Krebber, A., Horn, U., Knüpfer, U., Wenderoth, R., Nieba, L., Proba, K., and Riesenberg, D. (1997) in Antibody Engineering, A Practical Approach (Mc Cafferty, J., Hoogenboom, H. R., and Chiswell, D. J., Eds.) pp 203-252, Oxford University Press, New York.
33. Pace, C. N. (1990) Trends Biotechnol. 8, 93-98.
34. Lindner, P., Bauer, K., Krebber, A., Nieba, L., Kremmer, E., Krebber, C., Honegger, A., Klinger, B., Mocikat, R., and Plückthun, A. (1997) Biotechniques 22, 140-149.
35. Schmid, F. V. (1997) in Protein Structure, A Practical Approach (Creighton, T. F., Ed.) 2nd ed., pp 261-297. Oxford University Press, New York.
36. Cowgill, R. W. (1967) Biochim. Biophys. Acta 140, 37-41.
37. Yasui, H., Ito, W., and Kurosawa, Y. (1994) FEBS Lett. 353, 143-146.
38. Pantoliano, M. W., Bird, R. E., Johnson, S., Asel, E. D., Dodd, S. W., Wood, J. F., and Hardman, K. D. (1991) Biochemistry 30, 10117-10125.
39. Nieba, L., Honegger, A., Krebber, C., and Plückthun, A. (1997) Protein Eng. 10, 435-444.
40. Freund, C., Gehring, P., Holak, T. A., and Plückthun, A. (1997) FEBS Lett. 407, 42-46.
41. Freund, C, Honegger, A., Hunziker, P., Holak, T. A., and Plückthun, A. (1996) Biochemistry 35, 8457-8464.
42. Mayr, E. M., Jaenicke, R., and Glockshuber, R. (1997) J. Mol. Biol. 269, 260-269.
43. Ramsay, G., and Freire, E. (1990) Biochemistry 29, 8677-8683.
44. Edge, V., Allewell, N. M., and Sturtevant, J. M. (1988) Biochemistry 27, 8081-8087.
45. Brandts, J. F., Hu, C. Q., and Lin, L. N. (1989) Biochemistry 28, 8588-8596.