Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system

Journal of Immunological Methods

Volumn 201, Issue 1, 1997, Pages 35-55

  Krebber, Anke ^a   Bornhauser, Susanne ^a   Burmester, Jörg ^a   Honegger, Annemarie ^a   Willuda, Jörg ^a   Bosshard, Hans Rudolf ^a   Plückthun, Andreas ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

antibody library; monoclonal antibody; phage display; single chain Fv

Indexed keywords

AMPICILLIN; MESSENGER RNA; MONOCLONAL ANTIBODY; RECOMBINANT PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; DNA SEQUENCE; GENE EXPRESSION; GENETIC ENGINEERING; HYBRIDOMA; IMMUNIZATION; MOUSE; NONHUMAN; PHAGE DISPLAY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SPLEEN CELL;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; BASE SEQUENCE; BINDING SITES, ANTIBODY; CLONING, MOLECULAR; DNA PRIMERS; GENES, IMMUNOGLOBULIN; GENETIC VECTORS; HYBRIDOMAS; IMMUNOGLOBULIN VARIABLE REGION; MICE; MOLECULAR SEQUENCE DATA; PEPTIDE LIBRARY; POLYMERASE CHAIN REACTION; RECOMBINANT FUSION PROTEINS; SPLEEN; STRUCTURE-ACTIVITY RELATIONSHIP;

ANIMALIA;

EID: 0031032155     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(96)00208-6     Document Type: Article

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