Nucleotide exchange and excision technology (NExT) DNA shuffling: A robust method for DNA fragmentation and directed evolution

Nucleic Acids Research

Volumn 33, Issue 13, 2005, Pages 1-9

  Müller, Kristian M ^a   Stebel, Sabine C ^a   Knall, Susanne ^a   Zipf, Gregor ^b   Bernauer, Hubert S ^b   Arndt, Katja M ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

^b ATG Biosynthetics GmbH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CHLORAMPHENICOL ACETYLTRANSFERASE; DEOXYURIDINE TRIPHOSPHATE PYROPHOSPHATASE; DNA FRAGMENT; NUCLEOTIDE DERIVATIVE; PIPERIDINE; POLYACRYLAMIDE GEL; PRIMER DNA; TAQ POLYMERASE; THYMIDINE; URACIL; URACIL DNA GLYCOSYLTRANSFERASE; DEOXYRIBONUCLEASE (PYRIMIDINE DIMER); DEOXYURIDINE PHOSPHATE; DEOXYURIDINE TRIPHOSPHATE; DNA DIRECTED DNA POLYMERASE; NUCLEOTIDE; PIPERIDINE DERIVATIVE; PYRIMIDINE NUCLEOTIDE; THYMIDINE 5'-TRIPHOSPHATE; THYMIDINE TRIPHOSPHATE; TLI POLYMERASE;

ACCURACY; AMINO ACID SEQUENCE; ARTICLE; COMPUTER PROGRAM; DNA SHUFFLING; EXCISION REPAIR; FEASIBILITY STUDY; FRAGMENTATION REACTION; GENE AMPLIFICATION; GENE LIBRARY; GENE MUTATION; GENE TECHNOLOGY; METHODOLOGY; NICK END LABELING; NONHUMAN; NUCLEOTIDE EXCHANGE AND EXCISION TECHNOLOGY; POLYMERASE CHAIN REACTION; PREDICTION; PRIORITY JOURNAL; PROTEIN DEGRADATION; CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; GENETICS; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS;

CHLORAMPHENICOL O-ACETYLTRANSFERASE; DEOXYRIBONUCLEASE (PYRIMIDINE DIMER); DEOXYURACIL NUCLEOTIDES; DIRECTED MOLECULAR EVOLUTION; DNA FRAGMENTATION; DNA SHUFFLING; DNA-DIRECTED DNA POLYMERASE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE LIBRARY; NUCLEOTIDES; PIPERIDINES; POLYMERASE CHAIN REACTION; SOFTWARE; TAQ POLYMERASE; THYMINE NUCLEOTIDES;

EID: 27144471889     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gni116     Document Type: Article

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