Mutant library construction in directed molecular evolution: Casting a wider net

Molecular Biotechnology

Volumn 34, Issue 1, 2006, Pages 55-68

  Wang, Tian Wen ^a   Zhu, Hu ^a   Ma, Xing Yuan ^a   Zhang, Ting ^a   Ma, Yu Shu ^a   Wei, Dong Zhi ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Directed molecular evolution; DNA shuffling; Error prone PCR; Mutator strain; Oligonucleotide based mutagenesis; Somatic hypermutation

Indexed keywords

CELLS; DNA; ENZYMES; MOLECULAR BIOLOGY; MUTAGENESIS; MUTAGENS;

DNA SHUFFLING; MUTATOR STRAIN; OLIGONUCLEOTIDE-BASED MUTAGENESIS; SOMATIC HYPERMUTATION (SHM);

GENETIC ENGINEERING;

MUTANT PROTEIN;

B LYMPHOCYTE; DNA SHUFFLING; GENE LIBRARY; GENE SEQUENCE; GENETIC RECOMBINATION; HIGH THROUGHPUT SCREENING; MOLECULAR EVOLUTION; MUTAGENESIS; MUTATOR GENE; POLYMERASE CHAIN REACTION; REVIEW; SOMATIC HYPERMUTATION;

ANIMALS; CLONING, MOLECULAR; DIRECTED MOLECULAR EVOLUTION; EVOLUTION, MOLECULAR; GENE LIBRARY; GENETIC ENGINEERING; HUMANS; MUTAGENESIS; POLYMERASE CHAIN REACTION;

EID: 33748339568     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/MB:34:1:55     Document Type: Review

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