Improved Catalytic Efficiency and Active Site Modification of 1,4-β-D-Glucan Glucohydrolase A from Thermotoga neapolitana by Directed Evolution

Journal of Biological Chemistry

Volumn 279, Issue 12, 2004, Pages 11495-11502

  McCarthy, James K ^a,b   Uzelac, Aleksandra ^a   Davis, Diane F ^a   Eveleigh, Douglas E ^a  

^a The State University of New Jersey   (United States)

^b UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; ENZYMES; ESCHERICHIA COLI; GLUCOSE; HYDROLYSIS; OLIGOMERS; SUBSTRATES;

CATALYTIC EFFICIENCY; MUTANTS;

BIOCHEMISTRY;

AMINO ACID; ASPARAGINE; CELLOBIOSE; DISACCHARIDE; GLUCAN 1,4 BETA GLUCOSIDASE; ISOLEUCINE; THREONINE;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; ENZYME ACTIVE SITE; ENZYME ASSAY; ENZYME MODIFICATION; ESCHERICHIA COLI; EVOLUTION; HYDROLYSIS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PAENIBACILLUS; PAENIBACILLUS POLYMYXA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN STRUCTURE; THERMOSTABILITY; THERMOTOGA; THERMOTOGA NEAPOLITANA;

AMINO ACID SEQUENCE; BASE SEQUENCE; BINDING SITES; CATALYSIS; DIRECTED MOLECULAR EVOLUTION; DNA PRIMERS; GLUCAN 1,4-BETA-GLUCOSIDASE; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY, AMINO ACID; THERMOTOGA MARITIMA;

ESCHERICHIA COLI; FELIS CATUS; NEGIBACTERIA; PAENIBACILLUS; PAENIBACILLUS POLYMYXA; POLYMYXA; THERMOTOGA; THERMOTOGA NEAPOLITANA;

EID: 1642445607     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M305642200     Document Type: Article

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