Defining the binding site of homotetrameric R67 dihydrofolate reductase and correlating binding enthalpy with catalysis

Biochemistry

Volumn 43, Issue 23, 2004, Pages 7403-7412

  Strader, Michael Brad ^a   Chopra, Shaileja ^a   Jackson, Michael ^a   Smiley, R Derike ^a   Stinnett, Lori ^a   Wu, Jun ^a   Howell, Elizabeth E ^a  

^a UNIVERSITY OF TENNESSEE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALORIMETRY; CATALYSIS; ENTHALPY; ENZYMES; GENES; MUTAGENESIS; TERNARY SYSTEMS; TITRATION;

BINDING SITES; ISOTHERMAL TITRATION CALORIMETRY (ITC); MUTATIONS;

BIOCHEMISTRY;

ALANINE; DIHYDROFOLATE REDUCTASE; DIHYDROFOLIC ACID; GLUTAMINE; GLYCINE; ISOLEUCINE; LYSINE; NICOTINAMIDE; PTERIDINE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SERINE; THREONINE; TRIMETHOPRIM; TYROSINE; VALINE;

ANTIBIOTIC RESISTANCE; ARTICLE; CALORIMETRY; CATALYSIS; COMPLEX FORMATION; CONFORMATIONAL TRANSITION; ENTHALPY; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME KINETICS; ENZYME MECHANISM; ENZYME STRUCTURE; ENZYME SUBSTRATE; ENZYME TERNARY COMPLEX; GENE MUTATION; GENETIC CONSERVATION; ISOTHERMAL TITRATION CALORIMETRY; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; TITRIMETRY;

AMINO ACIDS; BINDING SITES; CATALYSIS; DIMERIZATION; HYDROGEN-ION CONCENTRATION; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; NADP; PROTEIN STRUCTURE, QUATERNARY; TETRAHYDROFOLATE DEHYDROGENASE; THERMODYNAMICS;

EID: 2942595927     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi049646k     Document Type: Article

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