Breaking symmetry: Mutations engineered into R67 dihydrofolate reductase, a D2 symmetric homotetramer possessing a single active site pore

Biochemistry

Volumn 41, Issue 52, 2002, Pages 15664-15675

  Smiley, R Derike ^a   Stinnett, Lori G ^a   Saxton, Arnold M ^a   Howell, Elizabeth E ^a  

^a UNIVERSITY OF TENNESSEE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HOMOTETRAMERS;

AGENTS; BACTERIA; CATALYSIS; GENES; MUTAGENESIS;

ENZYMES;

ANTIINFECTIVE AGENT; DIHYDROFOLATE REDUCTASE; DIHYDROFOLIC ACID; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE DIHYDROFOLATE COMPLEX; PLASMID DNA; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TETRAMER; TRIMETHOPRIM; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; ARTICLE; BINDING SITE; CATALYSIS; COMPLEX FORMATION; CONFORMATIONAL TRANSITION; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; GENE DUPLICATION; GENE INSERTION; GENE MUTATION; GENETIC ENGINEERING; GENETIC LINKAGE; LIGAND BINDING; MOLECULAR EVOLUTION; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; R67 DHFR GENE; REACTION OPTIMIZATION; STEADY STATE;

AMINO ACID SUBSTITUTION; BINDING SITES; CIRCULAR DICHROISM; GENE DOSAGE; GENES, BACTERIAL; GENES, SYNTHETIC; GLUTAMINE; HISTIDINE; KINETICS; MOLECULAR MIMICRY; MUTAGENESIS, SITE-DIRECTED; NADP; R FACTORS; TANDEM REPEAT SEQUENCES; TETRAHYDROFOLATE DEHYDROGENASE;

ESCHERICHIA COLI; INSERTION SEQUENCES; NEGIBACTERIA;

EID: 0037207139     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi026676j     Document Type: Article

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