Structural insights into the evolution of an antibody combining site

Science

Volumn 276, Issue 5319, 1997, Pages 1665-1669

  Wedemayer, Gary J ^a,b   Patten, Phillip A ^a,b,d   Wang, Leo H ^a,b   Schultz, Peter G ^a,b,c   Stevens, Raymond C ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b LAWRENCE LIVERMORE NATIONAL LABORATORY   (United States)

^c HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^d MAXYGEN INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HAPTEN; IMMUNOGLOBULIN F(AB) FRAGMENT;

AMINO ACID SUBSTITUTION; ANTIBODY COMBINING SITE; ANTIBODY STRUCTURE; ANTIGEN BINDING; ARTICLE; BINDING SITE; CONFORMATIONAL TRANSITION; CRYSTAL STRUCTURE; ENTHALPY; EVOLUTION; GERM LINE; HYDROGEN BOND; IMMUNE RESPONSE; IMMUNE SYSTEM; PRIORITY JOURNAL; SOMATIC MUTATION;

ANTIBODIES, CATALYTIC; ANTIBODY AFFINITY; ANTIBODY DIVERSITY; ANTIGEN-ANTIBODY COMPLEX; ANTIGEN-ANTIBODY REACTIONS; BINDING SITES; BINDING SITES, ANTIBODY; CRYSTALLOGRAPHY, X-RAY; EVOLUTION, MOLECULAR; HAPTENS; HYDROGEN BONDING; IMMUNOGLOBULIN FAB FRAGMENTS; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY;

EID: 0030821164     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.276.5319.1665     Document Type: Article

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59. We thank I. Wilson, B. Spiller, and B. Santarsiero for helpful comments on the manuscript. Supported by DOE contract DEACO3765F00098 (P.G.S.), NIH grant R01 AI39089 (R.C.S.), and the Howard Hughes Medical Institute (P.G.S.). We thank the Stanford Synchrotron Radiation Laboratory for Synchrotran beamtime. The coordinates have been deposited in the protein data bank with accession numbers 1AJ7and2RCS.