A glutamine 67→histidine mutation in homotetrameric R67 dihydrofolate reductase results in four mutations per single active site pore and causes substantial substrate and cofactor inhibition

Protein Engineering

Volumn 10, Issue 12, 1997, Pages 1415-1424

  Park, Heonyong ^a   Bradrick, Thomas D ^a   Howell, Elizabeth E ^a  

^a UNIVERSITY OF TENNESSEE   (United States)

Author keywords

Folate; Isothermal titration calorimetry; NADPH; R plasmid; Site directed mutagenesis

Indexed keywords

BACTERIAL ENZYME; DIHYDROFOLATE REDUCTASE; FOLIC ACID; GLUTAMINE; HISTIDINE; MUTANT PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TETRAMER; DIHYDROFOLIC ACID; DRUG DERIVATIVE; ENZYME INHIBITOR; HISTAMINE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING KINETICS; BINDING SITE; CALORIMETRY; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME INHIBITION; ENZYME SUBSTRATE COMPLEX; LIGAND BINDING; PRIORITY JOURNAL; STRUCTURE ACTIVITY RELATION; CHEMISTRY; ESCHERICHIA COLI; GENETICS; KINETICS; MACROMOLECULE; METABOLISM; SITE DIRECTED MUTAGENESIS; THERMODYNAMICS;

BINDING SITES; CALORIMETRY; CATALYSIS; ENZYME INHIBITORS; ESCHERICHIA COLI; FOLIC ACID; GLUTAMINE; HISTAMINE; KINETICS; MACROMOLECULAR SUBSTANCES; MUTAGENESIS, SITE-DIRECTED; NADP; STRUCTURE-ACTIVITY RELATIONSHIP; TETRAHYDROFOLATE DEHYDROGENASE; THERMODYNAMICS;

EID: 0031282924     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/10.12.1415     Document Type: Article

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