Rapid evolution of β-glucuronidase specificity by saturation mutagenesis of an active site loop

Journal of Biological Chemistry

Volumn 279, Issue 25, 2004, Pages 26462-26468

  Geddie, Melissa L ^a   Matsumura, Ichiro ^a  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALGORITHMS; CLONING; DNA; ESCHERICHIA COLI; MUTAGENESIS; VARIATIONAL TECHNIQUES;

MOLECULAR RECOGNITION; PROTEIN ENGINEERS; T4 DNA LIGASE;

PROTEINS;

4 NITROPHENYL BETA DEXTRO XYLOPYRANOSIDE; AMINO ACID; BETA GLUCURONIDASE; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; PYRANOSIDE; UNCLASSIFIED DRUG; XYLAN 1,4 BETA XYLOSIDASE;

ARTICLE; DNA SHUFFLING; ENZYME ACTIVITY; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENETIC RECOMBINATION; MUTAGENESIS; MUTATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS;

ALGORITHMS; BINDING SITES; DNA; DNA PRIMERS; ESCHERICHIA COLI; GLUCURONIDASE; KINETICS; MODELS, GENETIC; MODELS, MOLECULAR; MUTAGENESIS, INSERTIONAL; MUTATION; PLASMIDS; POLYMERASE CHAIN REACTION; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINATION, GENETIC; SEQUENCE ANALYSIS, DNA; SUBSTRATE SPECIFICITY; XYLOSIDASES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 2942739036     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M401447200     Document Type: Article

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