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Biomolecular Engineering

Volumn 22, Issue 1-3, 2005, Pages 73-79

Whole plasmid mutagenic PCR for directed protein evolution

(2) Matsumura, Ichiro a Rowe, Lori A a

a EMORY UNIVERSITY SCHOOL OF MEDICINE (United States)

Author keywords

Beta galactosidase; Directed evolution; Random mutagenesis

Indexed keywords

BIOASSAY; CLONING; DNA; ESCHERICHIA COLI; GENES; MUTAGENESIS; OPTIMIZATION;

DNA LIGASE; IN VIVO GENETIC SELECTION; POLYMERASE CHAIN REACTION (PCR); PROTEIN FUNCTION;

ENZYMES;

4 NITROPHENYLGALACTOSIDE; 5 BROMO 4 CHLORO 3 INDOLYL BETA DEXTRO GALACTOPYRANOSIDE; BETA GALACTOSIDASE; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; PYRANOSIDE; RESTRICTION ENDONUCLEASE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE ISOLATION; GENETIC SELECTION; GENETIC STABILITY; GENETIC VARIABILITY; MOLECULAR CLONING; MUTAGENESIS; MUTATION RATE; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN PURIFICATION;

EID: 18044363663 PISSN: 13890344 EISSN: None Source Type: Journal
DOI: 10.1016/j.bioeng.2004.10.004 Document Type: Article

Times cited : (25)

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