Hepatocyte culture as an in vitro model for evaluating the hepatotoxicity of food-borne toxicants and microbial pathogens: A review

Toxicology Mechanisms and Methods

Volumn 13, Issue 2, 2003, Pages 111-119

  Sahu, Saura C ^a  

^a CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

Food Toxicants; Hepatocytes; Hepatotoxicity; Liver Toxicity; Microbial Pathogens

Indexed keywords

AFLATOXIN B1; CARCINOGEN; CHEMICAL COMPOUND; COLCHICINE; DRUG; ETACRYNIC ACID; FOOD ADDITIVE; HERBACEOUS AGENT; HETEROCYCLIC AMINE; MUTAGENIC AGENT; MYCOTOXIN; PARACETAMOL; TOXIC SUBSTANCE; TOXIN; TROGLITAZONE;

ANIMAL CELL; CORRELATION ANALYSIS; FOOD; GENOMICS; HUMAN; HUMAN CELL; IN VITRO STUDY; LIVER CELL CULTURE; LIVER TOXICITY; MICROBIOLOGICAL EXAMINATION; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEOMICS; QUALITATIVE ANALYSIS; REVIEW; TOXICITY TESTING; VIRULENCE;

ANIMALIA; EQUIDAE;

EID: 0038510079     PISSN: 15376516     EISSN: 15376524     Source Type: Journal    
DOI: 10.1080/15376510309839     Document Type: Review

References (163)

1. Abdul-Hussain, S. K., and Mehendale, H. M. 1991. Studies of the age-dependent effects of galactosamine in primary rat hepatocyte cultures. Toxicol. Appl. Pharamacol. 107:504-513.
2. Amacher, D. E., and Martin, B. 1997. Tetracyclin-induced steatosis in primary canine hepatocytes. Fund. Appl. Toxicol. 40:256-263.
3. Amman, P., Laethem, C., and Kedderis, G. 1998. Choloroform-induced cytolethality in mouse and rat hepatocytes. Toxicol. Appl. Pharmacol. 149:217-225.
4. Arechabala, B., Coiffard, C., Rivalland, P., Coiffard, L., and Roeck-Holtzhauer, Y. 1999. Comparison of cytotoxicity of various surfactants tested on normal human fibrobalst cultures using neutral red test, MTT assay and LDH release. J. Appl. Toxicol. 19:163-165.
5. Ashida, H., Shiotani, B., Adachi, H., Kanazawa, K., and Danno, G. 1998. Tryptophan pyrolysis products Trp-P-1 and Trp-P-2 induce apoptosis in rat hepatocytes. Biosci. Biotech. Biochem. 62:2283-2287.
6. Barsig, J., and Kaufman, S. H. 1997. The mechanism of cell death in Listeria monocytogenes - infected murine macrophages is distinct from apoptosis. Infect. Immunity 65:4075-4081.
7. Barsig, J., Flesch, I. and Kaufmann, S. 1998. Hepatic cells as chemokine producers in murine listeriosis. Immunobiology 199:87-104.
8. Bayliss, M. K., Bell, J., Jenner, W., Park, G., and Wilson, K. 1999. Utility of hepatocytes to model species difffrences in the metabolism of loxtidine and to predict pharmacokinetic parameters in rat, dog and man. Xenobiotica 29:253-268.
9. Beamand, J. A., Price, R., Cunningham, M., and Lake, B. 1993. Culture of liver slices. Food Chem. Toxicol. 31:137-147.
10. Bhagawandeen, S. B., Apte, M., Manwarring, L., and Dickeson, J. 1987. Endotoxin-induced necrosis in rats. J. Pathol. 151:47-53.
11. Bigger, C. B., Brasky, K., and Lanford, R. 2001. DNA microarray analysis of chimpanzee liver during acute resolving hepatitis C virus infection. J. Virol. 75:7059-7066.
12. Blazka, M. E., and Shaikh, Z. A. 1991. Differences in cadmium and mercury uptakes by hepatocytes. Toxicol. Appl. Pharamacol. 110:355-363.
13. Borenfreund, E., and Puerner, J. 1985. Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol. Lett. 24: 119-124.
14. Boury, N. M., and Czuprynski, C. J. 1995. Listeria monocytogenes infection increases neutrophil adhesion and damage to murine hepatocyte cell line in vitro. Immunol. Lett. 46:111-116.
15. Casini, A., Giorli, M., Gilfor, D., and Farber, J. 1982. Mechanisms of cell injury in the killing of cultured hepatocytes by bromobenzene. J. Biol. Chem. 257:6721-6728.
16. Castell, J. V., Gomez-Lechon, M. J., Ponsoda, X., and Bort, R. 1997. The use of cultured hepatocytes to investigate mechanisms of drug hepatotoxicity. Cell Biol. Toxicol. 13:331-338.
17. Chen, H., Wu, H., Fon, C., Chen, P., Lai, M., and Chen, D. 1998. Longterm culture of hepatocytes from human adults. J. Biomed. Sci. 5:435-440.
18. Chesne, C., Guyomard, C., Fautrel, A., Poullain, M., Fremond, B., De Jong, H., and Guillouzo, A. 1993. Viability and function in primary cultures of adult hepatocytes from various animal species and human beings after cryopreservation. Hepatology 18:406-414.
19. Clark, F., and Reed, R. 1992. Chaparral-induced toxic hepatitis. JAMA 268:3295-3296.
20. Conlan, J. W., and North, R. J. 1991. Neutrophil-mediated disolution of host cells as defense strategy against facultative intracellular bacterium. J. Exp. Med. 174:741-744.
21. Conlan, J. W., and North, R. J. 1992. Early pathogenesis of infection in the liver with the facultative intracellular bacteria Listeria monocytogenes, Francisella tularensis and Salmonella typimurium involves lysis of infected hepatocytes by leukocytes. Infect. Immun. 60:5164-5171.
22. Coulet, M., Eeckhoutte, C., Sutra, J., Hoogenboom, A., Kuiper, H., Castell, J., Alvinerie, M., and Galtier, P. 1998. Comparative metabolism of thiabendazole in cultured hepatocytes from rats, rabbits, calves, pigs and sheep. J. Agric. Food Chem. 46:742-748.
23. Cummings, C. A., and Relman, D. A., 2000. Using DNA microarrays to study host-microbe interactions. Emerg. Infect. Dis. 6:513-523.
24. Davila, J. C., and Acosta, D. 1991. Changes in glutathione and cellular energy as potential mechanisms of papaverine-induced hepatotoxicity in vitro. Toxiol. Appl. Pharmacol. 108:28-36.
25. Davila, J. C., Davis, P. J., and Acosta, D. 1990. Cytotoxic effects of papaverine in liver cell culture. In Vitro Toxicol. 3:229-241.
26. Davila, J. C., Lenherr, A., and Acosta, D. 1989. Protective effects of flavonoids on drug-induced hepatotoxicity in vitro. Toxicology 57:267-286.
27. Davila, J. C., and Morris, D. L. 1999. Analysis of cytochrome P450 and phase Iconjugating enzymes expression in rat hepatocytes. In Vitro Cell. Dev. Biol. Anim. 35:120-130.
28. De Maria, N., Colantoni, A., Liu, G., Rogers, B., Thiel, D., and Floyed, R. 1996. Association between reactive oxygen species and disease activity in chronic hepatitis C. Free Radic. Biol. Med. 21:291-295.
29. Ding, W. X., Shen, H., and Ong, C. 2001. Critical role of reactive oxygen species in microcystin-induced cytoskeleton disruption in primary cultured hepatocytes. Toxicol. Environ. Health. 64:507-519.
30. Donato, M. T., Castell, J., and Gomez-Lechon, M. 1994. Cytochrome P450 activities in pure and cocultured rat hepatocytes: effects of model inducers. In Vitro Cell Dev. Biol. 30A:825-832.
31. Dunn, J. C., Tompkins, R., and Yarmush, M. 1992. Hepatocytes in collagen sandwich. J. Cell Biol. 116:1043-1053.
32. Dvorak, Z., Ulrichova, J., Pichard-Garcia, L., Modriansky, M., and Maurel, P. 2002. Comparative effect of colchicine and colchiceine on cytotoxicity and CYP gene expression in primary human hepatocytes. Toxicol. In Vitro 16:219-227.
33. El-Sakka, S., Salem, E., and Abdel-Rahman, M. 2002. In vitro hepatotoxicity of alachlor and its byproducts. J. Appl. Toxicol. 22:31-35.
34. Fau, D., Lekehal, M., Farrell, G., Feldman, G., Haouzi, D., and Pessayre, D. 1997. Diterpenoids from germander, an herbal medicine, induce apoptosis in rat hepatocytes. Gastroenterology 113:1334-1346.
35. Ferrini, J., Picard, L., Domergue, J., and Maurel, P. 1997. Long-term primary cultures of adult human hepatocytes. Chem. Biol. Interact. 107:31-45.
36. Frenzel, J., Richter, J., and Eschrich, K. 2002. Fructose inhibits apaoptosis induced by reoxygenation in rat hepatocytes. Biochim. Biophys. Acta 1542:82-94.
37. Fukuda, I., Tanamoto, K., Kanegasaki, S., Yajima, Y., and Goto, Y. 1989. Deacylation of bacterial lipopolysaccharide in rat hepatocytes in vitro. Br. J. Exp. Pathol. 70:267-274.
38. Galle, P. R., Hagelstein, J., Kommerell, B., Volkmann, M., Schranz, P., and Zentgraf, H. 1994. In vitro experimental infection of primary human hepatocytes with hepatitis B virus. Gastroenterology 106:664-673.
39. Garcia-Gasca, T., Fatell, S., Villa-Trevino, S., and Mejia, E. G. 1998. Effects of carotenoids against genotoxicity of diethylnitrosamine on rat hepatocytes. Toxicol. In Vitro 12:691-698.
40. Gardner, C. R., Heck, D., Yang, C., Thomas, P., Laskin, J., and Laskin, D. 1998. Role of nitric oxide in acetaminophen-induced hepatotoxicity in rat. Hepatology 27:748-754.
41. Gasbarrini, A., Colantoni, A., Masetti, M., Massari, I., Pola, P., and Bernardi, M. 1997. Intermittent anoxia reduces oxygen free radicals formation during reoxygenation in rat hepatocytes. Free Radic. Biol. Med. 23:1067-1072.
42. Gebhardt, R. 1997. Antioxidant and protective properties of extracts from leaves of the artichoke against oxidative stress in cultured rat hepatocytes. Toxicol. Appl. Pharmacol. 144:279-286.
43. 1, in rats. Carcinogenesis 12:1247-1251.
44. Gelderblom, W. C. A., Cawood, M., Snyman, S., Vleggar, R., and Marasas, W. 1993. Structure-activity relationship of fumonisins in a short-term carcinogenesis and cytotoxicty assays. Food Chem. Toxicol. 31:407-414.
45. Gomez-Lechon, M. J., Montoya, A., Lopez, P., Donato, T., Larrauri, A., and Castell, J. V. 1988. The potential use of cultured hepatocytes in predicting hepatotoxicity of xenobiotics. Xenobiotica 18:725-735.
46. Gray, T. J. B., Lake, B., Beamand, J., Foster, J., and Gangoli, S. 1983. Peroxisome proliferation in primary cultures of rat hepatocytes. Toxicol. Appl. Pharamacol. 67:15-25.
47. Green, C. E., Segal, H., and Byard, J. 1981. Metabolism, cytotoxicity, and genotoxicity of pyrrolizidine alkaloid senecionine in cultures of rat hepatocytes. Toxicol. Appl. Pharmacol. 60:176-185.
48. Gregory, S. H., and Wing, E. 1993. IFN-gamma inhibits replication of Listeria monocytogenes in hepatocytes. J. Immunol. 151:1401-1409.
49. Gregory, S. H., Sagnimeni, A., and Wing, E. 1996a. Bacteria in the blood stream are trapped in the liver and killed by immigrating neutrophils. J. Immunol. 157:2514-2520.
50. Gregory, S. H., Jiang, X., and Wing, E. J. 1996b. Lympokine-activated killer cells lyse Listeria-infected hepatocytes. J. Infect. Dis. 174:1073-1079.
51. Guengerich, F. P. 1991. Oxidation of toxic and carcinogenic chemicals by human cytochrome P450 enzymes. Chem. Res. Toxicol. 4:391-407.
52. Guguen-Guillouzo, C., Clement, B., Baffet, G., Beaumont, C., Glaise, D., and Guillouzo, A. 1983. Maintenance and reversibility of active albumin secretion by adult rat hepatocytes cocultured with other liver epithelial cell type. Exp. Cell Res. 143:47-54.
53. Guguen-Guillouzo, C., and Guillouzo, A. 1986. Methods of preparations of adult and fetal hepatocytes. In Isolated and Cultured Hepatocytes, eds. A. Guillouzo and C. Guguen-Guillouzo, 1-12. London: John Libbey Eurotext, London.
54. Guyomard, C., Rialland, L., Fremond, B., Chesne, C., and Guillouzo, A. 1996. Influence of alginate gel entrapment and cryopreservation on survival and xenobiotic metabolism capacity of rat hepatocytes. Toxicol. Appl. Pharamacol. 141:349-356.
55. Haidara, K., Morel, I., Abalea, V., Gascon, M., and Denizeau, F. 2002. Mechanisms of tert-butylhydroperoxide - induced apoptosis in rat hepatocytes. Biochim. Biophys. Acta 1542:173-185.
56. Hamilton, G. A., Westmoreland, C., and George, E. 2001. Effects of medium composition on morphology and function of rat hepatocytes cultured as spheroids and monolayers. In Vitro Cell Dev. Biol. Anim. 37:656-667.
57. 1-treated rat hepatocytes and Fischer 344 rats. Carcinogenesis 19:1451-1458.
58. Harries, H. M., Fletcher, S., Duggan, C., and Baker, V. 2001. The use of genomics technology to invetsigate gene expression changes in cultured human liver cells. Toxicol. In Vitro 15:399-405.
59. Hill, D. A., and Roth, R. A. 1998. Alphs-napthylisothiocyanate causes neutrophils to release factors that are cytotoxic to hepatocytes. Toxicol. Appl. Pharmacol. 148:169-175.
60. Hill, D. A., Jean, P. A., and Roth, R. A. 1999. Bile duct epithelial cells exposed to alpha-naphthylisothiocyanate produce a factor that causes neutrophildependent hepatocellular injury in vitro. Toxicol. Sci. 47:118-125.
61. Howes, A. J., Chan, V., and Caldwell, J. 1990. Structure-specificity of genotoxicity of some alkenylbenzenes in rat hepatocytes. Food Chem. Toxicol. 28:537-542.
62. Hsu, C., Quistad, G., and Casida, J. 1998. Phosphine-induced oxidative stress in Hepa 1c1c7 cells. Toxicol. Sci. 46:204-210.
63. Inoue, S., Itagaki, S., and Amano, F. 1995. Intracellular killing of Listeria monocytogenes in J774. 1 macrophage-like cell line and lipopolysaccaride(LPS)resistant mutant LPS 1916 cell line defective in generation of reactive oxygen intermediate after LPS treatment. Infect. Immun. 63:1876-1886.
64. Isom, H. C. 1980. DNA synthesis in isolated hepatocytes infected with herpesviruses. Virology 103:199-216.
65. Isom, H. C., Secott, T., Georgoff, I., Woodworth, C., and Mummaw, J. 1985. Maintenance of differentiated rat hepatocytes in primary culture. Proc. Natl. Acad. Sci. U S A 82:3252-3256.
66. 1-DNA binding in isolated rat hepatocytes. Cancer Res. 49:1357-1360.
67. Jimenez, I., Aracena, P., Letelier, M., Navarro, P., and Speisky, H. 2002. Chronic exposure of HepG2 cells to excess copper. Toxicol. In Vitro 16:167-175.
68. Jinno, H., Hatakeyama, N., Yoda, R., Nishimura, T., and Ando, M. 1999. Cytotoxic and porphyrinogenic effects of diphenyl ethers in cultured rat hepatocytes. Food Chem. Toxicol. 37:69-74.
69. Kainz, A., Cross, H., Freeman, S., Gescher, A., and Chipman, J. 1993. Effects of 1,1,-dichloroethylene on the functional viability of mouse hepatocytes. Fund. Appl. Toxicol. 21:140-148.
70. Katz, M., and Saibil, F. 1990. Herbal hepatitis. J. Clin. Gastroenterol. 12:203-206.
71. Kehrer, J. P. 1993. Free radicals as mediators of tissue injury and disease. Crit. Rev. Toxicol. 23:21-48.
72. Khalil, M., Shariat-Panahi, A., Toole, R., Ryder, T., Roberts, E., Hodgson, H., and Selden, C. 2001. Human hepatocyte cell lines proliferating as cohesive spheroid colonies in alginate markedly upregulate both synthetic and detoxificatory liver function. J. Hepatol. 297:68-77.
73. Knasmuller, S., Bresgen, N., Gelderblom, W., Zohrer, E., and Eckl, P. 1997. Genotoxic effects of three Fusarium mycotoxins in bacteria and in primary culture of rat hepatocytes. Mut. Res. 391:39-48.
74. Knowles, B. B., Howe, C., and Aden, D. 1980. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B suface antigen. Science 209:497-499.
75. Kono, Y., Yang, S., Letarte, M., and Roberts, E. 1995. Establishment of a human hepatocyte line derived from primary culture in a collagen gel sandwich culture system. Experiment. Cell Res. 221:478-485.
76. Kostrubsky, V. E., Sinclair, J. F., Ramachandran, V., Rose, K., Sinz, M., and Strom, S. C. 2000. The role of conjugation in hepatotoxicity of troglitazone in human and porcine hepatocyte cultures. Drug Metab. Dispos. 28:1192-1197.
77. Kremers, P., Roelandt, L., Todaro, A., Goffinet, G., and Thome, J. 1993. Use of cultured hepatocytes as an alternative method to study the effects of PCBs on living organisms. Toxic. In Vitro 7:433-437.
78. Kwiatkowski, A. P., Baker, T. K., and Klaunig, J. E. 1994. Comparison of glucocorticoid-mediated changes in the expression and function of rat hepatocytes gap junctional proteins. Carcinogenesis 15:1753-1757.
79. Lajarin, F., Rubio, G., Galvez, J., and Garcia-Penarrubia, P. 1996. Adhesion, invasion and itracellular replication of Salmonella typhimurium in a murine hepatocyte cell line. Microbiol. Pathogen. 21:319-329.
80. Lajarin, F., Rubio, G., Lorenzo, N., Gamiz, P., Hernandez-Caselles, T., and Garcia-Penarrubia, P. 1999. Implication of reactive oxygen species in the antibacterial activity against Salmonella typhimurium of hepatocyte cell line. Free Radic. Biol. Med. 27:1008-1018.
81. Lake, B. G., Beamand, J., Japenga, A., Renwick, A., and Davis, R. 1993. Induction of cytochrome P-450-dependent enzyme activities in cultured rat liver slices. Food Chem. Toxicol. 31:377-386.
82. Lash, L. H., Qian, D., Desai, K., Elfarra, A., Sicuri, A., and Parker, J. 1998. Glutathione conjugation of perchloroethylene in rat and mice in vitro. Toxicol. Appl. Pharamacol. 150:49-57.
83. Lavrijsen, K., Van Houdt, J., Van Dyke, D., Hendrickx, J., Bockx, M., Hurkmans, R., Le Jeune, L., Lavwers, W., and Heykants, J. 1992. Comparative metabolism of flunarizine in rat, dog and man: an in vitro study with subcellular liver fractions and isolated hepatocytes. Xenobiotica 22:815-816.
84. Le Bigot, J. F., Begue, J. M., Kiechel, J. R., and Guillouzo, A. 1987. Species differences. I. Metabolism of ketotifen in rat, rabbit and man: demonstration of similar pathways in vivo and in cultured hepatocytes. Life Sci. 40: 883-890.
85. LeCluyse, E. L. 2001. Human hepatocyte culture systems for the in vitro evaluation of cytochrome P450 expression and regulation. Eur. J. Pharmacol. Sci. 13:343-368.
86. Lekehal, M., Pessayer, D., Lereau, J., Moulis, C., Foureste, I., and Fau, D. 1996. Hepatotoxicity in the herbal medicine germander. Hepatology 24:212-218.
87. Li, A. P. 1997. Primary hepatocyte cultures as an in vitro experimental model for the evaluation of pharmacokinetic drug-drug interactions. Adv. Pharmacol. 43:103-130.
88. Li, A. P., Maurel, P., Gomez-Lechon, M., Cheng, L. C., and Jurima-Romet, M. 1997b. Preclinical evaluation of drug-drug interaction potential: present status of the application of primary human hepatocytes in the evaluation of cytochrome P450 induction. Chemico. Biol. Interact. 107:5-16.
89. Li, A. P., Reith, M., Rasmussen, A., Hall, S., Xu, L., Kaminski, D., and Cheng, L. 1997a. Primary human hepatocytes as a tool for the evaluation of structureactivity relationship in cytochrome P450 induction potential of xenobiotics. Chem. Biol. Interact. 107:17-30.
90. Li, A. P., Roque, M. A., Beck, D. J., and Kaminski, D. L. 1992. Isolation and culturing of hepatocytes from human livers. J. Tissue Cult. Meth. 14: 139-146.
91. Liu, S., Khmlani, L., Shapiro, R., Johnson, M., Liu, K., Geller, D., Watkins, S., Goyert, S., and Billiar, T. 1998. Expression of CD14 by hepatocytes: upregulation by cytokines during endotoxemia. Infect. Immun. 66: 5089-5098.
92. Long, R. M., and Moore, L. 1988. Biochemical evaluation of rat hepatocyte cultures as a model for carbon tetrachloride hepatotoxicity: comparative studies in vivo and in vitro. Toxicol. Appl. Pharmacol. 92:295-306.
93. Maheo, K., Morel, F., Kramer, H., Ketterer, B., and Guillouzo, A. 1997. Inhibition of cytochrome P450 and induction of glutathione S-transferase by sulforaphane in primary human and rat hepatocytes. Cancer Res. 57:3649-3652.
94. Mattes, W. B., and Li, A. P. 1997. Quantitative reverse transciptase/PCR assay for measurement of induction in cultured hepatocytes. Chem. Biol. Interact. 107:47-61.
95. McCord, J. M. 1995. Superoxide radical. Proceedings of the Society for Experimental Biology and Medicine 209:112-117.
96. McQueen, C. A., Way, B., Queener, S., Schlufer, G., and Willams, G. 1991. Study of potential in vitro and in vivo genotoxicity in hepatocytes of quinoline antibiotics. Toxicol. Appl. Pharamocol. 111:255-262.
97. Merrill, J. C., Beck, D. J., Kaminski, D. A., and Li, A. P. 1995. Polybrominated biphenyl induction of cytochrome P450 mixed-function oxidase activity in primary rat and human hepatocytes. Toxicology 99:147-152.
98. Michalopoulos, G. K. 1999. Hepatocyte primary cultures: Currently used systems and their applications for studies of hepatocyte growth and differentiation. Tissue Eng. Methods and Proto. 18:227-243.
99. Miura, Y., Sakisaka, S., Harada, M., Sata, M., and Tanikawa, K. 1995. Role of hepatocytes in direct clearance of lipopolysaccharide in rats. Gastroenterology 109:1969-1976.
100. Miyai, K. 1991. Structural organization of the liver. In Hepatotoxicology, eds. R. G. Meeks, S. D. Harrison, and R. J. Bull, 1-65. Boca Raton, FL: CRC.
101. Mochida, S., Masaki, N., Ohta, Y., Matsui, A., Ogata, I., and Fujiwara, K. 1992. In situ detection of oxidative stress in rat hepatocytes. J. Pathol. 167: 83-89.
102. Moridani, M. Y., Gelati, G., and O'Brien, P. 2002. Comparative quantitative structure activity relationship for flavonoids evaluated in isolated rat hepatocytes and HeLa tumor cells. Chem. Biol. Interact. 20:251-264.
103. Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65:55-63.
104. Muriel, P. 2000. Regulation of ntiric oxide synthesis in the liver. J. Appl. Toxicol. 20:189-195.
105. Nakagawa, Y., Suzuki, T., and Tayama, S. 2000. Metabolism and toxicity of benzophenone in isolated rat heaptocytes. Toxicology 156:27-36.
106. Norred, W. P., Platiner, R., Vesonder, R., Bacon, C., and Voss, K. 1992. Effeccts of selected secondary metabolites of Fuasrium moniliforme on unscheduled DNA synthesis by rat hepatocytes. Food Chem. Toxicol. 30:233-237.
107. Oldham, H. G., Standrind, P., Norman, S., Blake, T., Beattie, I., Cox, P., and Chenery, R. 1990. Metabolism of temelastin in hepatocytes from rat, dog, cynomologus monkey and man. Drug Metabol. Dispos. 18:146-152.
108. Olinga, P., Merema, M., Meijer, D., Slooff, M., and Groothuis, G. 1993. Human liver slices express the same lidocaine biotransformation rate as isolated human hepatocytes. Alternatives to Laboratory Animals 21:466-468.
109. Patel, T., Bronke, S., and Gores, G. 1994. Increases of intracellular magnesium promote glycodeoxycholate-induced apoptosis in rat hepatocytes. J. Clin. Invest. 94:2183-2192.
110. Patel, T., Roberts, L., Jones, B., and Gores, G. 1998. Dysregulation of apoptosis as a mechanism of liver disease: an overview. Semin. Liver Dis. 18:105-114.
111. Pathernik, S., Smihmid, J., Sauter, T., Schidberg, F., and Koebe, H. 1995. Metabolism of pimobendan in long-term human hepatocyte culture: in vivo - in vitro comparison. Xenobiotica 25:811-823.
112. Pichard, L., Fabre, I., Domegrue, J., Mourad, G., and Maurel, P. 1990. Cyclosporin A drug interactions: screening for inducers and inhibitors of cytochrome P450 in primary cultures of human hepatocytes. Drug Metab. Dispos. 18:595-606.
113. Plymale, D. R., and de la Iglesia, F. 1999. Acridine-induced subcellular and functional changes in isolated human hepatocytes in vitro. J. Appl. Toxicol 19:31-38.
114. Price, P. J., Samrock, R. L., Lobo-Alfonso, J., and Green, C. E. 1997. Retention of xenobiotic-inducible cytochrome P450 gene expression in hepatocytes. In Vitro Toxicol. 3:365-371.
115. Qu, B., Li, L., Wong, K., Tan, T., and Halliwell, B. 2001. Mechanism of clofibrate hepatotoxicity. Free Radic. Biol. Med. 31:659-669.
116. Reitman, F. A., Shertzer, H., and Berger, M. 1988. Toxicity of methylating agents in isolated hepatocytes. Biochem. Pharamacol. 37:3183-3188.
117. Richert, L., Price, S., Chesne, C., Maita, K., and Carmichael, P., 1996. Comparision of the induced hepatic peroxisome proleferation by the herbicide oxadiazon in vivo in rats, mice and dogs and in vitro in rat and human hepatocytes. Toxicol. Appl. Pharmacol. 141:35-43.
118. Rogers, H. W., Callery, M. P., Deck, B., and Unanue, E. R. 1996. Listeria monocytogenes induces aptosis of infected hepatocytes. J. Immunol. 156:679-684.
119. Rogiers, V., and Vercruysse, A. 1995. Rat hepatocyte culture and co-cultures in biotransformation studies of xenobiotics. Toxicology 99:193-208.
120. Rook, G. A. W., Steele, J., Umar, S., and Dockrell, H. M. 1985. A simple method for the solubilization of reduced NBT and its use as colorimetric assay for activation of human macrophages by gamma-interferon. J. Immunol. Methods 82:161-167.
121. Ruffa, M. J., Ferraro, G., Wagner, M., Calcagno, M., Campos, R., and Cavallaro, L. 2002. Cytotoxic effects of Argentine medicinal plant extracts on human hepatocellular carcinoma cell line. J. Ethnopharmacol. 79:335-339.
122. Sahu, S. C. 1990. Oncogenes, oncogenesis and oxygen radicals. Biomed. Environ. Sci. 3:183-201.
123. Sahu S. C. 1991. Role of oxygen free radicals in the molecular mechanisms of carcinogenesis: a review. J. Environ. Sci. Health C9:83-112.
124. Sahu, S. C., Flynn, T., Bradlaw, J., Roth, W., Barton, C., and Yates, J. 2001. Pro-oxidant effects of the flavonoid myricetin on rat hepatocytes in culture. Toxicol. Methods 11:277-283.
125. Salminen, W. F., Voellmy, R., and Roberts, S. 1995. Induction of hsp 70 in HepG2 cells in resposnse to hepatotoxicants. Toxicol. Appl. Pharmacol. 141:117-123.
126. Sandker, G., Vos, R., Delbressine, L., Sloof, M., Meijer, D., and Groothuis, G. 1994. Metabolism of three pharmacologically active drugs in isolated human and rat hepatocytes. Xenobiotica 24:143-155.
127. Sandy, M. S., Moldeus, P., Ross, D., and Smith, M. 1986. Role of redox cycling and lipid peroxidation in bipyridyl herbicide cytotoxicity. Biochem. Pharmacol. 35:3095-3101.
128. Scheers, E. M., Ekwall, B., and Dierickx, P. J. 2001. In vitro long-term cytotoxicity testing of 27 MEIC chemicals on HepG2 cells and comparison with acute human toxicity data. Toxicol. In Vitro 15:153-161.
129. Schwarz, K. B. 1996. Oxidative stress during viral infection: a review. Free Radic. Biol. Med. 21:641-649.
130. Seglen, P. O. 1976. Preparation of isolated rat liver cells. Methods Cell Biol. 13:29-83.
131. 1-induced cell injury in cultured rat hepatocytes. Toxicology 99:115-123.
132. 1. Free Radic. Biol. Med. 21:139-146.
133. Shen, W., Kamendulis, L., Ray, S., and Corcoran, G. 1992. Acetamine-induced cytotoxicity in cultured mouse hepatocytes. Toxicol. Appl. Pharamacol. 112:32-40.
134. Shiota, G., Maeta, Y., Mukpyama, T., Oyama, K., Yashima, K., Ito, H., Murawaki, Y., and Kawasaki, H. 2002. Effects of Sho-saiko-to on hepatocarinogenosis and 8-hydroxy-2′-deoxyguanosine formation. Hepatology 35:1125-1133.
135. Sina, J. F., Bean, C., Dysart, G., Taylor, V., and Bradley, M. 1983. Evaluation of alkaline elution/rat hepatocyte as a predictor of carcinogenic/mutagenic potential. Mutat. Res. 113:357-391.
136. Skett, P. 1994. Problems in using isolated and cultured hepatocytes for xenobiotic metabolism/metaboliism-based toxicity testing. Solutions? Toxicol. In Vitro 8:491-504.
137. Smith, P. F., Krack, G., McKee, R., Johnson, D., Hruby, V., Krumdieck, C., and Brendel, K. 1986. Maintenance of adult rat liver slices in dynamic organ culture. In Vitro 22:706-712.
138. Sneed, R. A., Grimes, S., Schultze, E., Brown, A., and Garney, P. 1997. Bacterial endotoxin enhances hepatotoxicity of allyl alcohol. Toxicol. Appl. Pharmacol. 144:77-87.
139. Spitzer, J. A. 1995. Active oxygen intermediates: beneficial or deleterious. Proc. Soc. Exp. Biol. Med. 209:102-103.
140. Stacey, N. H., and Klaassen, C. D. 1981a. Inhibition of lipid peroxidation without prevention of cellular injury in isolated rat hepatocytes. Toxicol. Appl. Pharmacol. 58:8-18.
141. Stacey, N. H., and Klaasssen, C. D. 1981b. Copper toxicity in isolated rat hepatocytes. Toxicol. Appl. Pharmacol. 58:211-220.
142. Starke, P. E., and Farber, J. 1985. Endogenous defenses against cytotoxicity of hydrogen peroxide in cultured rat hepatocytes. J. Biol. Chem. 260: 86-92.
143. Stedman, C. 2002. Herbal hepatotoxicity. Sem. Liver Dis. 22:195-206.
144. Susa, N., Ueno, S., Furukawa, Y., Ueda, J., and Sugiyama, M. 1997. Potent protective effect of melatonin on chromium VI)-induced DNA strand breaks in cultures of rat hepatocytes. Toxicol. Appl. Pharmacol. 144:377-384.
145. Sydenham, E. W., Thiel, P. G., Marasas, W. F., Shephard, G. S., Van Schalkyk, D. J., and Koch, K.R. 1990. Natural occurrence of some Fusarium mycotoxins in com from low and high esophageal cancer prevalence areas of South Africa. J. Agric. Food Chem. 38:1900-1903.
146. Tagawa, M., Kato, N., Yokosuka, O., Ohto, M., and Omata, M. 1995. Infection of human hepatocyte cell line with hepatitis C virus. J. Gastrol. Hepatol. 10:523-527.
147. Tateno, C., and Yoshizato, K. 1996. Long-term cultivation of adult rat hepatocytes that undergo multiple cell division. Am. J. Pathol. 148:383-392.
148. Thomas, L., DeFeo, B., Mariaani, M., and Van Rosum, G. 1989. Comparison of metabolic effects of carbon tetrachloride and 1,2-dichloroethane added in vitro to slices of rat liver. Toxicol. In Vitro 3:59-68.
149. Toyoda, Y., Tsuchida, A., Iwami, E., and Miwa, I. 2001. Toxic effect of troglitazone on cultured rat hepatocytes. Life Sci. 68:1867-1876.
150. Tran-Thi, T. A., Phillips, J., Falk, H., and Decker, K. 1995. Toxicity of D-galactosamine for rat hepatocytes in monolayer culture. Exp. Mol. Pathol. 42:89-116.
151. Tseng, T., Kao, E., Chu, C., Chou, F., Wu, H., and Wang, C. 1997. Protective effects of dried flower extracts of Hibiscus sabdariffa against oxidative stress in rat hepatocytes. Food Chem. Toxicol. 35, 1159-1164.
152. Uhl, M., Helma, C., and Knasmuller, S. 2000. Evaluation of the single-cell electrophoresis assay with HepG2 cells. Mutat. Res. 468:213-225.
153. Voskoboinik, I., Ooi, S., Drew, R., and Ahokas, J. 1997. Peroxisome proliferators increase the formation of BDPE-DNA adducts in rat hepatocytess. Toxicology 122:81-91.
154. Walker, T. M., Rhodes, P., and Westmoreland, C. 2000. The differential cytotoxicity of methotrexate in rat hepatocyte monolayer aand spheroid cultures. Toxicol. In Vitro 14:475-485.
155. Walum, E., Balls, M., Bianchi, V., Blauboer, B., Bolcsfoldi, G., Guillouzo, A., Moore, G., Oldand, L., Reinhardt, C., and Spielman, H. 1992. ECITTS: an integral approach to the application of in vitro test systems to the hazard assessment of chemicals. Alternatives to Laboratory Animals 20:406-428.
156. Wang, J. H., Redmond, H., Watson, R., and Bouchier-Hays, D. 1995. Role of lipopolysaccharide and tumor necrosis factor-alpha in induction of hepatocyte necrosis. Am. J. Physiol. 269:G297-G304.
157. 1. Food Chem. Toxicol. 36:497-503.
158. Williams, G. M., Laspia, M. F., and Dunkel, V. C. 1982. Reliability of the hepatocyte primaru culture DNA repair test in testing coded carcinogens and noncarcinogens. Mutat. Res. 97:359-370.
159. Wing, E. J., and Gregory, S. H. 2002. Listeria monocytogenes: clinical and experimental update. J. Infect. Dis. 185:S18-S24.
160. Wood, S., Maroushek, N., and Czupryski, C. J. 1993. Multiplication of Listeria monocytogenes in a murine hepatocyte cell line. Infect. Immun. 61:3068-3072.
161. Yamamoto, K., Masubuchi, Y., Narimatu, S., Kobayashi, S., and Horie, T. 2002a. Toxicity of ethacrynic acid in isolated rat hepatocytes. Toxicol. In Vitro 16:151-158.
162. Yamamoto, Y., Yamazaki, H., Ikeda, T., Watanabe, T., Iwabuchi, H., Nakajima, M., and Yokoi, T. 2002b. Formation of novel quinone epoxide metabolites of troglitazone with cytotoxicity to HepG2 cells. Drug. Metab. Dispos. 30:155-160.
163. Yang, C., Shen, H., and Ong, C. 1999. Protective effect of Ebselen against hydrogen peroxide-induced cytotoxicity and DNA damage in HepG2 cells. Biochem. Pharamacol. 57:273-279.