Protective effect of ebselen against hydrogen peroxide-induced cytotoxicity and DNA damage in HepG2 cells

Biochemical Pharmacology

Volumn 57, Issue 3, 1999, Pages 273-279

  Yang, Cheng Feng ^a   Shen, Han Ming ^a   Ong, Choon Nam ^a  

^a NONE

Author keywords

Cytotoxicity; DNA damage; Ebselen; HepG2 cells; Hydrogen peroxide

Indexed keywords

EBSELEN; HYDROGEN PEROXIDE; LACTATE DEHYDROGENASE;

ARTICLE; CELL GROWTH; CELL PROTECTION; CELL STRAIN HEPG2; CONTROLLED STUDY; CYTOTOXICITY; DNA DAMAGE; HEPATOMA CELL; HUMAN; HUMAN CELL; LIPID PEROXIDATION; PRIORITY JOURNAL;

AZOLES; CYTOPROTECTION; DNA DAMAGE; GROWTH INHIBITORS; HUMANS; HYDROGEN PEROXIDE; L-LACTATE DEHYDROGENASE; LIPID PEROXIDATION; ORGANOSELENIUM COMPOUNDS; TUMOR CELLS, CULTURED;

EID: 0032955320     PISSN: 00062952     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-2952(98)00299-8     Document Type: Article

References (20)

1. Ames B.N., Shigenaga M.K., Hagen T.M. Oxidants, antioxidants, and the degenerative diseases of aging. Proc Natl Acad Sci USA. 90:1993;7915-7922.
2. Parnham M.J., Leyck S., Graf E., Dowling E.J., Blake D.R. The pharmacology of ebselen. Agents and Actions. 32:1991;4-9.
3. Sies H. Ebselen, a selenoorganic compound as glutathione peroxidase mimic. Free Radical Biol Med. 14:1993;313-323.
4. Noguchi N., Gotoh N., Niki E. Effects of ebselen and probucol on oxidative modifications of lipid and protein of low density lipoprotein induced by free radicals. Biochim Biophys Acta. 1213:1994;176-182.
5. Narayanaswami V., Sies H. Antioxidant activity of ebselen and related selenoorganic compounds in microsomal lipid peroxidation. Free Radical Res Commun. 10:1990;237-244.
6. Lass A., Witting P., Stocker R., Esterbauer H. Inhibition of copper- and peroxyl radical-induced LDL lipid oxidation by ebselen Antioxidant actions in addition to hydroperoxide-reducing activity . Biochim Biophys Acta. 1303:1996;111-118.
7. Schoneich C., Narayanaswami V., Asmus K.D., Sies H. Reactivity of ebselen and related selenoorganic compounds with 1,2-dichloroethane radical cations and halogenated peroxyl radicals. Arch Biochem Biophys. 282:1990;18-25.
8. Sies H., Masumoto H. Ebselen as a glutathione peroxidase mimic and as a scavenger of peroxynitrite. Adv Pharmacol. 38:1997;229-246.
9. Batna A., Fuchs C., Spiteller G. Lipid peroxidation in presence of ebselen. Chem Phys Lipids. 87:1997;149-158.
10. Thomas C.E., Jackson R.L. Lipid hydroperoxid involvement in copper-dependent and independent oxidation of low density lipoproteins. J Pharmacol Exp Ther. 256:1990;1182-1188.
11. Sies H. Oxidative Stress. 1985;Academic Press, New York.
12. Halliwell B., Aruoma O.I. DNA damage by oxygen-derived species. FEBS Lett. 281:1991;9-19.
13. Hansen M.B., Nielsen S.E., Berg K. Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J Immunol Methods. 119:1989;203-210.
14. Shen H.M., Ong C.N., Shi C.Y. Involvement of reactive oxygen species in aflatoxin B1-induced cell injury in cultured rat hepatocytes. Toxicology. 99:1995;115-123.
15. Uchiyama M., Mihara M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem. 86:1978;271-278.
16. Singh N.P., McCoy M.T., Tice P.R., Schneider E.L. A simple technique for quantification of low level of DNA damage in individual cells. Exp Cell Res. 175:1988;184-191.
17. Anderson D., Yu T.W., Philips B.J., Schmezer P. The effects of various antioxidants and other modifying agents on oxygen-radical -generated DNA damage in human lymphocytes in the comet assay. Mut Res. 307:1994;261-271.
18. Shen H.M., Shi C.Y., Ong C.N. Detection of elevated reactive oxygen species level in cultured rat hepatocytes treated with aflatoxin B1. Free Radical Biol Med. 21:1996;139-146.
19. LeBel C.P., Ischiopoulos H., Bondy S.C. Evaluation of the probe 2′,7′-dichlorofluorescin as indicator of reactive oxygen species formation and oxidative stress. Chem Res Toxicol. 5:1992;227-231.
20. Halliwell B., Gutteridge J.M.C., Cross C.E. Free radicals, antioxidants, and human disease where are we now? J Lab Clin Med. 119:1992;598-620.