Influence of alginate gel entrapment and cryopreservation of survival and xenobiotic metabolism capacity of rat hepatocytes

Toxicology and Applied Pharmacology

Volumn 141, Issue 2, 1996, Pages 349-356

  Guyomard, Claire ^a   Rialland, Laure ^a   Fremond, Benjamin ^b   Chesne, Christophe ^a   Guillouzo, André ^b  

^a Technopole Atalante Villejean   (France)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

ALGINIC ACID; DRUG METABOLIZING ENZYME; ETHOXYRESORUFIN DEETHYLASE; LIPID; PROTEIN;

ANIMAL CELL; ARTICLE; CELL FUNCTION; CELL SURVIVAL; CELL VIABILITY; CONTROLLED STUDY; CRYOPRESERVATION; ENZYME ACTIVITY; GEL; LIVER CELL; MALE; NONHUMAN; RAT; XENOBIOTIC METABOLISM;

EID: 0030460927     PISSN: 0041008X     EISSN: None     Source Type: Journal    
DOI: 10.1006/taap.1996.0299     Document Type: Article

References (40)

1. Arnaout, W. S., Moscioni, A. D., Barbour, R. L., and Demetriou, A. A. (1990). Development of bioartificial liver: Bilirubin conjugation in Gunn rats. J. Surg. Res. 48, 379-382.
2. Bousquet, B., Fiet, J., Julien, R., Bon, R., and Dreux, C. (1965). Applications aux milieux biologiques de la réaction de l'urée avec la diacétylmonoxime sensibilisée par la thiosemicarbazide. Anal. Biol. Clin. 29, 415-422.
3. Bradford, M. (1976). A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
4. Burke, M., and Orrenius, S. (1978). The effect of albumin on the metabolism of ethoxyresorufin through O-deethylation and sulphate-conjugation using isolated rat hepatocytes. Biochem. Pharmacol. 27, 1533-1538.
5. Cai, Z., Shi, Z., Sherman, M., and Sun, A. M. (1989). Development and evaluation of a system of microencapsulation of primary rat hepatocytes. Hepatology 10, 855-860.
6. Chesné, C., and Guillouzo, A. (1988). Cryopreservation of isolated rat hepatocytes: A critical evaluation of freezing and thawing conditions. Cryobiology 25, 232-330.
7. Chesné, C., Gripon, P., and Guillouzo, A. (1988). Primary culture of cryopreserved rat hepatocytes. In Liver Cells and Drugs (A. Guillouzo, Ed.), Vol. 164, pp. 343-350. Les Editions INSERM & John Libbey Eurotext, Paris.
8. Chesné, C., Guyomard, C., Fautrel, A., Poullain, M. G., Frémond, B., De Jong, H., and Guillouzo, A. (1993). Viability and function in primary culture of adult hepatocytes from various animal species and human beings after cryopreservation. Hepatology 18, 406-414.
9. Demetriou, A. A., Rozga, J., Podesta, L., Lepage, E., Morsiani, E., Moscioni, A. D., Hoffman, A., McGrath, M., Kong, L., Rosen, H., Villamil, F., Woolf, G., Vierling, J., and Makowka, L. (1995). Early clinical experience with a hybrid bioartificial liver. Scand. J. Gastroenterol. 30(Suppl. 208), 111-117.
10. Diener, B., Abdel-Latif, H., Arand, M., and Oesch, F. (1995). Xenobiotic metabolizing enzyme activities and viability are well preserved in EDTA-isolated rat liver parenchymal cells after cryopreservation. Toxicol. Appl. Pharmacol. 130, 149-153.
11. Dixit, V., Darvasi, R., Arthur, M., Lewin, K., and Gitnick, G. (1993). Cryopreserved microencapsulated hepatocytes: Transplantation studies in Gunn rats. Transplantation 55, 616-622.
12. Dixit, V. (1995). Transplantation of isolated hepatocytes and their role in extrahepatic life support systems. Scand. J. Gastroenterol. 30(Suppl. 208), 101-110.
13. Dou, M., De Sousa, G., Lacarelle, B., Placidi, M., Lechéne, D. E., La Porte, P., Domingo, M., Lafont, H., and Rahmani, R. (1992). Thawed human hepatocytes in primary culture. Cryobiology 29, 454-469.
14. Frémond, B., Malandain, C., Guyomard, C., Chesné, C., Guillouzo, A., and Campion, J. P. (1993). Correction of bilirubin conjugation in the Gunn rat using hepatocytes immobilized in alginate gel beads as an extracorporeal bioartificial liver. Cell Transplant. 2, 453-460.
15. Griffith, O. W. (1980). Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. Anal. Biochem. 106, 207-212.
16. Guguen-Guillouzo, C., and Guillouzo A. (1983). Modulation of functional activities in cultured rat hepatocytes. Mol. Cell. Biochem. 53/54, 35-36.
17. Guguen-Guillouzo, C., and Guillouzo, A.(1986). Methods for preparation of adult and fetal hepatocytes. In Isolated and Cultured Hepatocytes (A. Guillouzo and C. Guguen-Guillouzo, Eds.), pp. 1-12. Les Editions INSERM and John Libbey Eurotext, Paris.
18. Guguen-Guillouzo, C., Clément, B., Baffet, G., Beaumont, C., Morel-Chany, E., Glaise D., and Guillouzo A. (1983). Maintenance and reversibility of active albumin secretion by adult rat hepatocytes co-cultured with another liver epithelial cell type. Exp. Cell Res. 143, 47-54.
19. Guillouzo, A., Morel, F., Fardel, O., and Meunier, B. (1993). Use of human hepatocyte cultures for drug metabolism studies. Toxicology 82, 209-219.
20. Guillouzo, A., and Chesné, C. (1996). Xenobiotic metabolism in epithelial cell cultures In Cell Culture Models of Epithelial Tissues: A Practical Approach (A. J. Shaw, Ed.), pp. 67-85. University Press, Oxford.
21. Koebe, H. G., Dunn, J. C., Tonner, M., Sterling, L. M., Hubel, A., Cravalho, E. G., Yarmush, M. L., and Tompkins, R. G. (1990). A new approach to the cryopreservation of hepatocytes in a sandwich culture configuration. Cryobiology 27, 576-584.
22. Labarca, C., and Paigen, K. (1980). A simple, rapid and sensitive DNA assay procedure. Anal. Biol. 102, 344-352.
23. Lawrence, J. N., and Benford, D. J. (1991). Development of an optimal method for the cryopreservation of hepatocytes and their subsequent monolayer culture. Toxicol In Vitro 5, 39-51.
24. Lim, F., and Sun, A. M. (1980). Microencapsulated islets as bioartificial endocrine pancreas. Science 210, 908-910.
25. Loretz, L. J., Li, A. P., Flye, M. W., and Wilson, A. C. (1989). Optimization of cryopreservation procedures for rat and human hepatocytes. Xenobiotica 19, 489-498.
26. Martinsen, A., Skjak-Braek, G., and Smidsrod, O. (1989). Alginate as immobilization material. I. Correlation between chemical and physical properties of alginate gel beads. Biotechnol. Bioeng. 33, 79-89.
27. Miura, Y., Akimoto, T., Kanazawa, H., and Yagi, K. (1986). Synthesis and secretion of protein by hepatocytes entrapped within calcium alginate. Artif. Org. 10, 460-465.
28. Miura, Y., Akimoto, T., Yoshikawa, N., and Yagi, K. (1990). Characterization of immobilized hepatocytes as liver support. Biomater. Artif. Cells Artif. Org. 18, 549-554.
29. Nyberg, S. L., Peshwa, M. V., Payne, W. D., Hu, W. S., and Cerra, F. B. (1993). Evolution of the bioartificial liver: The need for randomized clinical trials. Am. J. Surg. 166, 512-521.
30. Omura, T., and Sato, R. (1964). The carbon monoxide-binding pigment of liver microsomes. J. Biol. Chem. 239, 2370-2378.
31. Ponec, M., Weerheim, A., Kempenaar, J., Mommaas, A. M., and Nugteren, D. H. (1988). Lipid composition of cultured human keratinocytes in relation to their differentiation. J. Lipids Res. 29, 949-961.
32. Powis, G., Santone, K. S, Melder, D. C., Thomas, L., Moore, D. J., and Wilke, T. J. (1987). Cryopreservation of rat and dog hepatocytes for studies of xenobiotic metabolism and activation. Drug Metab. Dispos. 15, 826-232.
33. Shirai, Y., Hashimoto, K., Yamaji, H., and Kawahara H. (1988). Oxygen uptake rate of immobilized growing hybdridoma cells. Appl. Microbiol. Biotechnol. 29, 113-118.
34. Skajk-Braek, G., Murano, E., and Paoletti, S. (1989). Alginate as immobilization material. II. Determination of polyphenol contaminants by fluorescence spectroscopy, and evaluation of methods for their removal. Biotechnol. Bioeng. 33, 90-94.
35. Smidsrod, O., and Skajk-Braek, G. (1990). Alginate as immobilization matrix for cells. Trends Biotechnol. 3, 71-78.
36. Tamponnet, C., Boisseau, S., Lirsac, P. N., Barbotin, J. N., Poujeol, C., Lievremont, M., and Simonneau, M. (1990). Storage and growth of neuroblastoma cells immobilized in calcium-alginate beads. Appl. Microbiol. Biotechnol. 33, 442-447.
37. Tanaka, H., Matsumura, M., and Veliky, A. (1984). Diffusion characteristics of substrates in Ca-alginate gel beads. Biotechnol. Bioeng. 67, 138-140.
38. Tompkins, R. G., and Carter, E. A. (1988). Enzymatic function of alginate immobilized rat hepatocytes. Biotechnol. Bioeng. 31, 11-18.
39. Trinder, P. (1969). Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen. J. Clin. Pathol. 22, 158-161.
40. Utesch, D., Diener, B., Molitor, E., Oesch, F., and Platt, K. L. (1992). Characterization of cryopreserved rat liver parenchymal cells by metabolism of diagnostic substrates and activities of related enzymes. Biochem. Pharmacol. 44, 309-315.