Long-term cultivation of adult rat hepatocytes that undergo multiple cell divisions and express normal parenchymal phenotypes

American Journal of Pathology

Volumn 148, Issue 2, 1996, Pages 383-392

  Tateno, Chise ^a   Yoshizato, Katsutoshi ^a,b  

^a JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^b HIROSHIMA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ALBUMIN; ALPHA FETOPROTEIN; ASCORBIC ACID 2 PHOSPHATE; DIMETHYL SULFOXIDE; EPIDERMAL GROWTH FACTOR; NICOTINAMIDE;

ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL DIVISION; CELL PROLIFERATION; CONTROLLED STUDY; CULTURE MEDIUM; DESMOSOME; INTRAHEPATIC BILE DUCT; ITO CELL; LABELING INDEX; LIVER CELL CULTURE; LIVER PARENCHYMA; MALE; NONHUMAN; PEROXISOME; PRIORITY JOURNAL; RAT; STELLATE CELL; TIGHT JUNCTION;

ALBUMINS; ALPHA-FETOPROTEINS; ANIMALS; ASCORBIC ACID; CELL AGGREGATION; CELL CULTURE TECHNIQUES; CELL DIVISION; CULTURE MEDIA; DIMETHYL SULFOXIDE; EPIDERMAL GROWTH FACTOR; IMMUNOHISTOCHEMISTRY; LIVER; MALE; MICROSCOPY, ELECTRON; NIACINAMIDE; PHENOTYPE; RATS; RATS, INBRED F344; TIME FACTORS;

EID: 0030051251     PISSN: 00029440     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (26)

1. Sawada N, Lee G-H, Mochizuki Y, Ishikawa T: Active proliferation of mouse hepatocytes in primary culture under defined conditions as compared to rat hepatocytes. Jpn J Cancer Res 1988, 79:983-988
2. Wu JC, Merlino G, Cveklova K, Mosinger B, Fausto N: Autonomous growth in serum-free medium and production of hepatocellular carcinomas by differentiated hepatocyte lines that overexpress transforming growth factor α Cancer Res 1994, 54:5964-5973
3. McGowan JA, Strain AJ, Bucher NLR. DNA synthesis in primary cultures of adult rat hepatocytes in a defined medium: effects of epidermal growth factor, insulin, glucagon, and cyclic-AMP J Cell Physiol 1981, 108:353-363
4. Nakamura T, Nawa K, Ichihara A: Partial purification and characterization of hepatocyte growth factor from serum of hepatectomized rats. Biochem Biophys Res Commun 1984, 122 1450-1459
5. Nakamura T, Teramoto H, Tomita Y, Ichihara A. L-Proline is an essential amino acid for hepatocyte growth in culture Biochem Biophys Res Commun 1984, 122: 884-891
6. Houck KA, Zarnegar R, Muga SJ, Michalopoulos GK: Acidic fibroblast growth factor (HBGF-1) stimulates DNA synthesis in primary rat hepatocyte cultures J Cell Physiol 1990, 143:129-132
7. Mitaka T, Sattler CA, Sattler GL, Sargent LM, Pitot HC Multiple cell cycles occur in rat hepatocytes cultured in the presence of nicotinamide and epidermal growth factor. Hepatology 1991, 13:21-30
8. Yamamoto N, Imazato K, Masumoto A: Growth stimulation of adult rat hepatocytes in a primary culture by soluble factor(s) secreted from nonparenchymal liver cells. Cell Struct Funct 1989, 14:217-219
9. Fujinaga Y, Morozumi N, Sato K, Tokumitsu Y, Fujinaga K, Kondo Y, Ui M, Okajima F A pertussis toxin-sensitive GTP-binding protein plays a role in the G0-G1 transition of rat hepatocytes following establishment in primary culture. FEBS Lett 1989, 245 117-121
10. Paine AJ, Hockin LJ, Legg RF Relationship between the ability of nicotinamide to maintain nicotinamide-adenine dinucleotide in rat liver cell culture and its effect on cytochrome P-450. Biochem J 1979, 184 461-463
11. Inoue C, Yamamoto H Nakamura, T, Ichihara T, Okamoto H. Nicotinamide prolongs survival of primary cultured hepatocytes without involving loss of hepatocyte-specific functions J Biol Chem 1989, 264:4747-4750
12. Bissell DM, Guzelian PS: Ascorbic acid deficiency and cytochrome P-450 in adult rat hepatocytes in primary monolayer culture Arch Biochem Biophys 1979, 192 569-576
13. Senoo H, Tsukada Y, Sato T, Hata R. Co-culture of fibroblasts and hepatic parenchymal cells induces metabolic changes and formation of a three-dimensional structure Cell Biol Int Rep 1989, 13 197-206
14. Bucher NLR, Patel U, Cohen S: Hormonal factors and liver growth. Adv Enzyme Regul 1978, 16 205-213
15. Eckl PM, Whitcomb WR, Michalopoulos G, Jirtle RL: Effects of EGF and calcium on adult parenchymal hepatocyte proliferation. J Cell Physiol 1987, 132:363-366
16. Isom HC, Secott T, Georgoff I, Woodworth C, Mummaw J Maintenance of differentiated rat hepatocytes in primary culture. Proc Natl Acad Sci USA 1985, 82:3252-3256
17. Staecker JL, Sattler CA, Pitot HC. Sodium butyrate preserves aspects of the differentiated phenotype of normal adult rat hepatocytes in culture. J Cellular Physiol 1988, 135:367-376
18. Mitaka T, Sattler GL, Pitot HC, Mochizuki Y. Characteristics of small cell colonies developing in primary cultures of adult rat hepatocytes. Virchows Arch B Cell Pathol 1992, 62:329-335
19. Seglen PO Preparation of isolated rat liver cells. Methods Cell Biol 1976, 13:29-83
20. Kreamer BL, Staecker JL, Sawada N, Sattler GL, Hsia MTS, Pitot HC Use of a low-speed isodensity Percoll centrifugation method to increase the viability of isolated rat hepatocyte preparations In Vitro 1986, 22 201-211
21. Li CY, Lam KW, Yam LT Esterases in human leukocytes. J Histochem Cytochem 1973, 21 1-12
22. Goldfischer S Essner E: Peroxidase activity in peroxisomes (microbodies) of acatalasemic mice J Histochem Cytochem 1970, 18 482-489
23. Bisgaard HC, Nagy P, Ton PT, Hu Z, Thorgeirsson SS: Modulation of keratin 14 and α-fetoprotein expression during hepatic oval cell proliferation and liver regeneration. J Cell Physiol 1994, 159:475-484
24. Guguen-Guillouzo C, Clément B, Baffet G, Beaumont C, Morel-Chany E, Glaise D, Guillouzo A. Maintenance and reversibility of active albumin secretion by adult rat hepatocytes co-cultured with another liver epithelial cell type. Exp Cell Res 1983, 143:47-54
25. Senoo H, Hata R: Extracellular matrix regulates and L-ascorbic acid 2-phosphate further modulates morphology, proliferation, and collagen synthesis of perisinusoidal stellate cells. Biochem Biophys Res Commun 1994, 200:999-1006
26. Loréal O, Levavasseur F, Fromaget C, Gros D, Guillouzo A, Clément B: Cooperation of Ito cells and hepatocytes in the deposition of an extracellular matrix in vitro. Am J Pathol 1993, 143:538-544