Comparison of cytotoxicity of various surfactants tested on normal human fibroblast cultures using the neutral red test, MTT assay and LDH release

Journal of Applied Toxicology

Volumn 19, Issue 3, 1999, Pages 163-165

  Arechabala, B ^a   Coiffard, C ^a   Rivalland, P ^a   Coiffard, L J M ^a   De Roeck Holtzhauer, Y ^a  

^a Lab Pharm Indust de Cosmetologie   (France)

Author keywords

Cytotoxicity; Fibroblast cultures; LDH release; MTT assay; Neutral red test; Surfactants

Indexed keywords

ANIONIC SURFACTANT; BENZETHONIUM CHLORIDE; CATIONIC SURFACTANT; LACTATE DEHYDROGENASE; NEUTRAL RED; POLYSORBATE 60; POLYSORBATE 80; SURFACTANT; TETRAZOLE DERIVATIVE; TEXAPON; THIAZOLE DERIVATIVE; TRITON X 100;

ARTICLE; BIOASSAY; COLORIMETRY; COMPARATIVE STUDY; CYTOTOXICITY; ENZYME ASSAY; ENZYME RELEASE; FIBROBLAST CULTURE; HUMAN; HUMAN CELL; LC 50; PRIORITY JOURNAL; STATISTICAL ANALYSIS;

BENZETHONIUM; CELLS, CULTURED; FIBROBLASTS; HUMANS; L-LACTATE DEHYDROGENASE; NEUTRAL RED; OCTOXYNOL; POLYSORBATES; SODIUM DODECYL SULFATE; SURFACE-ACTIVE AGENTS; TETRAZOLIUM SALTS; THIAZOLES; TOXICITY TESTS;

EID: 0032904831     PISSN: 0260437X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1099-1263(199905/06)19:3<163::AID-JAT561>3.0.CO;2-H     Document Type: Article

References (15)

1. P. Rivalland, L. Coiffard and Y. de Roeck-Holtzhauer, Determination of the cytotoxicity of sodium laurylsulfate on reconstituted skin. Cell Biol. Toxicol. 10, 436 (1994).
2. P. Rivalland, K. Vié, L. Coiffard and Y. De Roeck-Holtzhauer, Cytotoxicity tests of antibacterial agents on human fibroblasts cultures. Pharm. Acta Helv. 69, 159-162 (1994).
3. M. M. Bason, V. Gordon and H. I. Maibach, Skin irritation in vitro assays. Int. J. Dermatol. 30, 1295-1365 (1991).
4. J. Giridhar and D. Acosta, Evaluation of cytotoxicity potential of surfactants using primary rat keratinocytes culture as an in vitro cutaneous model. Toxicol. in Vitro 6, 33-45 (1993).
5. M. Adolphe, A. Guillouzo and F. Marano, Toxicologie cellulaire. In In vitro Méthodes et applications. Inserm, Paris (1995).
6. E. Borenfreund and J. A. Puerner. Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol. Lett. 24, 119-124 (1985).
7. E. Borenfreund, H. Babich and N. Martin-Alguacil, Comparisons of two in vitro cytotoxicity assays - the neutral red (NR) and tetrazolium MTT tests. Toxicol. in Vitro 2, 1-6 (1988).
8. H. Babich and E. Borenfreund, Cytotoxic effects of food additives and pharmaceuticals on cells in culture as determined with the neutral red assay. J. Pharm. Sci. 79, 592-594 (1990).
9. R. M. Lasarow, R. Rivkah Isseroff and E. C. Gomez. Quantitative in vitro assessment of phototoxicity by a fibroblast-neutral red assay. J. Invest. Dermatol. 98, 725-729 (1992).
10. C. Verhulst, C. Coiffard, L. J. M. Coiffard, P. Rivalland and Y. De Roeck-Holtzhauer, In vitro correlation between two colorimetric assays and the pyruvic acid consumption by fibroblasts cultured to determine the sodium laurylsulfate cytotoxicity. J. Pharmacol. Toxicol. 39, 143-145 (1998).
11. T. Mosmann, Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65, 55-63 (1983).
12. F. Denizot and R. Lang, Rapid colorimetric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Immunol. Methods 89, 271-277 (1986).
13. D. Triglia, S. S. Braa, T. Donnely, I. Kidd and G. K. Naughton, A three dimensional human dermal model substrate for in vitro toxicological studies. In Vitro Toxicol. 8, 351-362 (1991).
14. X. Ponsoda. Measurement of intracellular LDH activity in 96-well cultures: a rapid and automated assay for cytotoxicity studies. J. Tissue Culture Methods 13, 21-24 (1991).
15. G. S. Hsieh and D. Acosta, Dithranol-induced cytotoxicity in primary cultures of rat epidermal keratinocytes. Toxicol. Appl. Pharmacol. 107, 16-26 (1991).