Long-term primary cultures of adult human hepatocytes

Chemico-Biological Interactions

Volumn 107, Issue 1-2, 1997, Pages 31-45

  Ferrini, Jean Bernard ^a   Pichard, Lydiane ^a   Domergue, Jacques ^a,b   Maurel, Patrick ^a  

^a CNRS   (France)

^b HÔPITAL SAINT ELOI   (France)

Author keywords

CYP induction; Hepatocyte; Human; Long term culture

Indexed keywords

2,3,7,8 TETRACHLORODIBENZO PARA DIOXIN; CYCLOSPORIN A; CYTOCHROME P450 ISOENZYME; RIFAMPICIN;

ADULT; AGED; CONFERENCE PAPER; CULTURE MEDIUM; DRUG METABOLISM; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; IMMUNOBLOTTING; LIVER CELL CULTURE; MALE; PROTEIN DETERMINATION; PROTEIN EXPRESSION;

AGED; AGED, 80 AND OVER; ALPHA 1-ANTITRYPSIN; APOLIPOPROTEIN A-I; APOLIPOPROTEIN B-100; APOLIPOPROTEINS B; CELLS, CULTURED; CYCLOSPORINE; CYTOCHROME P-450 ENZYME SYSTEM; ELECTRON TRANSPORT; FEMALE; FIBRINOGEN; GENE EXPRESSION REGULATION; HUMANS; ISOENZYMES; LIVER; MALE; MIDDLE AGED; OXIDATION-REDUCTION; PHENOTYPE; PLASMINOGEN; SERUM ALBUMIN; TIME FACTORS;

PAPIO HAMADRYAS;

EID: 0030710503     PISSN: 00092797     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0009-2797(97)00072-0     Document Type: Conference Paper

References (26)

1. V. Rogiers, Cultures of human hepatocytes in in vitro pharmaco-toxicology, in: V. Rogiers, W. Sonck, E. Shephard, A. Vercruysse (Eds.), Human Cells in In Vitro Pharmaco-toxicology. Present Status within Europe, VUB Press, Brussels, 1993, pp. 77-115.
2. A. Guillouzo, Hepatotoxicity, in: J.M. Frazier (Ed.), In Vitro Toxicity Testing. Application to Safety Evaluation, Marcel Dekker, New York, 1992, pp. 45-83.
3. V. Rogiers, B. Blaauboer, P. Maurel, I. Phillips, L. Shephard, Hepatocyte-based in vitro models and their application in pharmacotoxicology, Toxicol. In Vitro 9 (1995) 685-694.
4. P. Maurel, The use of adult human hepatocytes in primary culture and other in vitro systems to investigate drug metabolism in man, Adv. Drug Del. Rev. 22 (1996) 105-132.
5. J. Muntane-Relat, J.C. Ourlin, J. Domergue, P. Maurel, Differential effects of cytokines on the inducible expression of CYP1A1, CYP1A2, and CYP3A4 in human hepatocytes in primary culture, Hepatology 22 (1995) 1143-1153.
6. J. Greuet, L. Pichard, J.C. Ourlin, C. Bonfils, J. Domergue, P. Le Treut, P. Maurel, Effect of cell density and epidermal growth factor on the inducible expression of CYP3A and CYP1A genes in human hepatocytes in primary culture, Hepatology 25 (1997) 1168-1175.
7. C. Guguen-Guillouzo, B. Clément, G. Baffet, C. Beaumont, E. Morel-Chagny, D. Glaise, A. Guillouzo, Maintenance and reversibility of active albumin secretion by adult rat hepatocytes co-cultured with another liver epithelial cell type, Exp. Cell Res. 143 (1983) 47-54.
8. A. Guillouzo, P. Beaune, M.N. Gascoin, J.M. Begue, J.P. Campion, F.P. Guengerich, C. Guguen-Guillouzo, Maintenance of cytochrome P450 in cultured adult human hepatocytes, Biochem. Pharmacol. 34 (1985) 2991-2995.
9. C. Lerche, A. Fautrel, P.M. Shaw, D. Glaise, F. Ballet, A. Guillouzo, L. Corcos, Regulation of the major detoxication functions by phenobarbital and 3-methylcholanthrene in co-cultures of rat and liver epithelial cells, Eur. J. Biochem. 244 (1997) 98-106.
10. E.G. Schuetz, D. Li, C.J. Omiecinski, U. Muller-Eberhard, H.K. Kleinman, B. Elswick, P.S. Guzelian, Regulation of gene expression in adult rat hepatocytes cultured on a basement membrane matrix, J. Cell. Physiol. 134 (1988) 309-323.
11. J.C.Y. Dunn, R.G. Tompkins, M.L. Yarmush, Long-term in vitro function of adult hepatocytes in a collagen sandwich configuration, Biotechnol. Prog. 7 (1991) 237-245.
12. H.G. Koebe, S. Pahernik, P. Eyer, F.W. Schildberg, Collagen gel immobilization: A useful cell culture technique for long-term metabolic studies on human hepatocytes, Xenobiotica 24 (1994) 95-107.
13. J.Z. Tong, P. De Lagausie, V. Furlan, T. Cresteil, O. Bernard, F. Alvarez, Long-term culture of adult rat hepatocyte spheroids, Exp. Cell Res. 200 (1992) 326-332.
14. H.C. Isom, T. Secott, I. Georgoff, C. Woodworth, J. Mummaw, Maintenance of differentiated rat hepatocytes in primary culture, Proc. Natl. Acad. Sci. USA 82 (1985) 3252-3256.
15. R.E. Lanford, K.D. Carey, L.E. Estlack, G.C. Smith, R.V. Hay, Analysis of plasma protein synthesis in long-term primary cultures of baboon hepatocytes maintained in a serum-free medium, In Vitro Cell. Dev. Biol. 25 (1989) 174-182.
16. L. Pichard, I. Fabre, G. Fabre, J. Domergue, B. Saint Aubert, G. Mourad, P. Maurel, Cyclosporin A drug interactions. Screening for inducers and inhibitors of cytochrome P450 (cyclosporin A oxidase) in primary cultures of human hepatocytes and in liver microsomes, Drug Metab. Dispos. 18 (1990) 595-606.
17. L. Pichard, I. Fabre, M. Daujat, J. Domergue, H. Joyeux, P. Maurel, Effect of corticosteroids on the expression of cytochromes P450 and on cyclosporin A oxidase activity in primary cultures of human hepatocytes, Mol. Pharmacol. 41 (1992) 1047-1055.
18. R. Curi-Pedrosa, M. Daujat, L. Pichard, J.C. Ourlin, P. Clair, L. Gervot, P. Lesca, J. Domergue, H. Joyeux, G. Fourtanier, P. Maurel, Omeprazole and lansoprazole are mixed inducers of CYP1A and CYP3A in human hepatocytes in primary culture, J. Pharmacol. Exp. Ther. 269 (1994) 384-392.
19. H.C. Isom, I. Georgoff, Quantitative assay for albumine-producing liver cells after simian virus 40 transformation of rat hepatocytes maintained in chemically defined medium, Proc. Natl. Acad. Sci. USA 81 (1984) 6378-6382.
20. L.S. Friedman, P. Martin, S.J. Munoz, Liver function tests and the objective evaluation of the patient with liver disease, in: Zakim, D. and Boyer, T.D., Eds, Hepatology a textbook of liver disease, vol. I, 3rd edn., Saunders, Philadelphia, 1996, pp. 791-833.
21. F. Morel, P.H. Beaune, D. Ratanasavanh, J.P. Flinois, C.S. Yang, F.P. Guengerich, A. Guillouzo, Expression of cytochrome P450 enzymes in cultured human hepatocytes, Eur. J. Biochem. 191 (1990) 437-444.
22. E.G. Schuetz, J.D. Schuetz, S.C. Strom, M.T. Thompson, R.A. Fisher, D.T. Molowa, D. Li, P.S. Guzelian, Regulation of human liver cytochromes P450 in family 3A in primary and continuous culture of human hepatocytes, Hepatology 18 (1993) 1254-1262.
23. A.P. Li, A. Rasmussen, L. Xu, D.L. Kaminski, Rifampicin induction of lidocaine metabolism in cultured human hepatocytes, J. Pharmacol. Exp. Ther. 274 (1995) 673-677.
24. P. Blanc, H. Etienne, M. Daujat, I. Fabre, F. Zindy, J. Domergue, C. Astre, B. Saint Aubert, H. Michel, P. Maurel, Mitotic responsiveness of cultured adult human hepatocytes to epidermal growth factor, transforming growth factor a, and human serum, Gastroenterology 102 (1992) 1340-1350.
25. P. Blanc, J. Liautard, J. Greuet, J.P. Daures, J.M. Fabre, D. Larrey, H. Michel, P. Maurel, Effect of antiulcer drugs on DNA synthesis in adult normal human hepatocytes in culture, Hepatology 22 (1995) 814-819.
26. E. Lai, J.E. Darnell, Transcription control in hepatocytes: A window on development, Trends Biochem. Sci. 16 (1991) 427-430.