Approaches for deciphering the structural basis of low temperature enzyme activity

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1543, Issue 2, 2000, Pages 417-433

  Sheridan, Peter P ^a   Panasik, Nicholas ^a   Coombs, Jonna M ^a   Brenchley, Jean E ^a  

^a PENNSYLVANIA STATE UNIVERSITY   (United States)

Author keywords

Directed evolution; Enzyme; Psychrophile; Structure

Indexed keywords

COLD TOLERANCE; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME MECHANISM; ENZYME STRUCTURE; GENETIC DRIFT; MUTAGENESIS; NONHUMAN; PHYLOGENY; PRIORITY JOURNAL; PROKARYOTE; REVIEW; STRUCTURE ANALYSIS; TEMPERATURE DEPENDENCE; TEMPERATURE SENSITIVITY;

ALPHA-AMYLASE; BACTERIA; COLD; CRYSTALLOGRAPHY, X-RAY; DIRECTED MOLECULAR EVOLUTION; ENZYME STABILITY; ENZYMES; PHYLOGENY; PROTEIN CONFORMATION; PROTEIN ENGINEERING;

EID: 0034731469     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4838(00)00237-5     Document Type: Review

References (90)

1. Ecological aspects of Antarctic microbiology
2. Distribution of cultivated and uncultivated cyanobacteria and Chloroflexus-like bacteria in hot spring microbial mats
3. Genetic diversity of archaea in deep-sea hydrothermal vent environments
4. Citromicrobium bathyomarinum, a novel aerobic bacterium isolated from deep-sea hydrothermal vent plume waters that contains photosynthetic pigment-protein complexes
5. T and Gelidibacter sp. strain IC158
6. Microbial reduction of Fe(III) in acidic sediments: Isolation of Acidiphilium cryptum JF-5 capable of coupling the reduction of Fe(III) to the oxidation of glucose
7. Psychromonas antarcticus gen. nov., sp. nov., a new aerotolerant anaerobic, halophilic psychrophile isolated from pond sediment of the McMurdo Ice Shelf, Antarctica
8. Dissimilatory reduction of Fe(III) by thermophilic bacteria and archaea in deep subsurface petroleum reservoirs of Western Siberia
9. Thermococcus barophilus sp. nov., a new barophilic and hyperthermophilic archaeon isolated under high hydrostatic pressure from a deep-sea hydrothermal vent
10. Microbial life at low temperatures: Ecological aspects
11. Psychrophilic microorganisms and their cold-active enzymes
12. Molecular adaptations in psychrophilic bacteria: Potential for biotechnological applications
13. Psychrophilic enzymes: Molecular basis of cold adaptation
14. Psychrophilic enzymes: A thermodynamics challenge
15. Cold-adapted enzymes
16. Toward a molecular understanding of cold activity of enzymes from psychrophiles
17. Cold-adapted enzymes: From fundamentals to biotechnology
18. Towards a natural system of organisms: Proposal for the domains archaea, bacteria, and eucarya
19. Methanogenium frigidum sp. nov., a psychrophilic, H2-using methanogen from Ace Lake, Antarctica
20. Directed evolution study of temperature adaptation in a psychrophilic enzyme
21. Protein stability and molecular adaptation to extreme conditions
22. Crystallin ribonuclease A loses function below the dynamical transition at 220 K
23. Crystal structures of the psychrophilic alpha-amylase from Alteromonas haloplanctis in its native form and complexed with an inhibitor
24. Structural adaptations of the cold-active citrate synthase from an Antarctic bacterium
25. Structural basis for cold adaptation
26. Triosephosphate isomerase (TIM) of the psychrophilic bacterium Vibrio marinus
27. Structures of the psychrophilic Alteromonas haloplanctis α-amylase give insights into cold adaptation at a molecular level
28. On the nature of global classification
29. Within- and between-species DNA sequence variation and the 'footprint' of natural selection
30. Hyperthermostable mutants of Bacillus licheniformis alpha-amylase: Multiple amino acid replacements and molecular modelling
31. Amino acid substitutions enhancing thermostability of Bacillus polymyxa beta-glucosidase A
32. Enhancement of thermostability and catalytic efficiency of AprP, an alkaline protease from Pseudomonas sp., by the introduction of a disulfide bond
33. Increasing the thermostability of D-xylose isomerase by introduction of a proline into the turn of a random coil
34. Disruption of an ionic network leads to accelerated thermal denaturation of D-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic bacterium Thermotoga maritima
35. Cloning and thermostability of Taq I endonuclease isoschizomers from Thermus species SM32 and Thermus filiformis Tok6A1
36. Ion pairs involved in maintaining a thermostable structure of glutamate dehydrogenase from a hyperthermophilic archaeon
37. Hydrophobic interactions of Val75 are critical for oligomeric thermostability of inorganic pyrophosphatase from Bacillus stearothermophilus
38. Engineering of a cold-adapted protease by sequential random mutagenesis and a screening system
39. The hyperthermostable inoleglycerol phosphate synthase from Thermotoga maritima is destabilized by mutational disruption of two solvent-exposed salt bridges
40. Primary structure, expression, and site-directed mutagenesis of inorganic pyrophosphatase from Bacillus stearothermophilus
41. Hydrophobic interaction at the subunit interface contributes to the thermostability of 3-isopropylmalate dehydrogenase from an extreme thermophile, Thermus thermophilus
42. Enhanced thermal stability of Clostridium beijerinckii alcohol dehydrogenase after strategic substitution of amino acid residues with prolines from the homologous thermophilic Thermoanaerobacter brockii alcohol dehydrogenase
43. Subtilisin from psychrophilic Antarctic bacteria: Characterization and site-directed mutagenesis of residues possibly involved in the adaptation to cold
44. Searching sequence space
45. Design by directed evolution
46. DNA shuffling of a family of genes from diverse species accelerates directed evolution
47. Rapid evolution of a protein in vitro by DNA shuffling
48. DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution
49. Random priming in vitro recombination: An effective tool for directed evolution
50. Incremental truncation as a strategy in the engineering of novel biocatalysts
51. Identification of amino acid substitutions that alter the substrate specificity of TEM-1 beta-lactamase
52. Tuning the activity of an enzyme for unusual environments: Sequential random mutagenesis of subtilisin E for catalysis in dimethylformamide
53. Molecular evolution by staggered extension process (StEP) in vitro recombination
54. Directed evolution of a thermostable esterase
55. A structural view of evolutionary divergence
56. Functional and nonfunctional mutations distinguished by random recombination of homologous genes
57. Exploring nonnatural evolutionary pathways by saturation mutagenesis: Rapid improvement of protein function
58. Cold adaptation of a mesophilic subtilisin-like protease by laboratory evolution
59. Purification, characterization, and nucleotide sequence of the thermolabile α-amylase from the Antarctic psychrotroph Alteromonas haloplanctis A23
60. Stability and structural analysis of α-amylase from the antarctic psychrophile Alteromonas haloplanctis A23
61. Thermodynamic stability of a cold-active α-amylase from the Antarctic bacterium Alteromonas haloplanctis
62. Purification and characterization of the heat-labile α-amylase secreted by the psychrophilic bacterium TAC 240B
63. Enzymes from cold-adapted microorganisms
64. A cold-adapted lipase of an Alaskan psychrotroph, Pseudomonas sp. strain B11-1: Gene cloning and enzyme purification and characterization
65. Nucleotide and derived amino acid sequence of the subtilisin from the antarctic psychrotroph Bacillus TA39
66. Cold adaptation of proteins
67. Cold adaptation of a mesophilic serine protease, subtilisin, by in vitro random mutagenesis
68. Properties of a subtilisin-like proteinase from a psychrotrophic Vibrio species
69. Production and properties of an extracellular metalloprotease from a psychrophilic Pseudomonas fluorescens
70. Preliminary crystal structure determination of the alkaline protease from the Antarctic psychrophile Pseudomonas aeruginosa
71. Properties of a cold-active protease from psychrotrophic Flavobacterium balustinum P104
72. Cold-active serine alkaline protease from the psychrotrophic bacterium Shewanella strain Ac10: Gene cloning and enzyme purification and characterization
73. Temperature adaptation of lactate dehydrogenase: Structural, functional and genetic aspects
74. Characterization of malate dehydrogenase from deep-sea psychrophilic Vibrio sp. strain no. 5710 and cloning of its gene
75. Cloning, sequencing and overexpression of the gene encoding malate dehydrogenase from the deep-sea bacterium Photobacterium species strain SS9
76. Archaeal cold-adapted proteins: Structural and evolutionary analysis of the elongation factor 2 proteins from psychrophilic, mesophilic and thermophilic methanogens
77. A RNA polymerase with transcriptional activity at 0°C from the Antarctic bacterium Pseudomonas syringae
78. Cloning and overexpression of the triosephosphate isomerase genes from psychrophilic and thermophilic bacteria
79. A thermolabile triosephosphate isomerase from the psychrophile Vibrio sp. strain ANT-300
80. Characterization of psychrotrophic microorganisms producing β-galactosidase activities
81. Characterization of a psychrotrophic Arthrobacter gene and its cold-active β-galactosidase
82. A novel Arthrobacter b-galactosidase with homology to eucaryotic β-galactosidases
83. Biochemical and phylogenetic analyses of a cold-active β-galactosidase from the lactic acid bacterium Carnobacterium piscicola BA
84. Characterization of a salt-tolerant family 42 β-galactosidase from a psychrophilic Antarctic Planococcus isolate
85. A cold-active glucanase from the ruminal bacterium Fibrobacter succinogenes S85
86. Sequencing and expression of the gene encoding a cold-active citrate synthase from an Antarctic bacterium, strain DS2-3R
87. Purification and properties of histidinol dehydrogenases from psychrophilic, mesophilic and thermophilic bacilli
88. Cold-adapted alanine dehydrogenases from two Antarctic bacterial stains: Gene cloning, protein characterization, and comparison with mesophilic and thermophilic counterparts
89. Purification and some characteristics of phosphatase of a psychrophile