Enhancement of thermostability and catalytic efficiency of AprP, an alkaline protease from Pseudomonas sp., by the introduction of a disulfide bond

Biochemical and Biophysical Research Communications

Volumn 221, Issue 3, 1996, Pages 631-635

  Ko, Jung Ho ^a   Jang, Won Hee ^a   Kim, Eun Kyung ^a   Lee, Hwanghee Biaise ^b   Park, Kee Don ^c   Chung, Jae Hoon ^a   Yoo, Ook Joon ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Chonnam National University   (South Korea)

^c UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PROTEINASE; BACTERIAL ENZYME; MUTANT PROTEIN; SERINE PROTEINASE; SYNTHETIC PEPTIDE;

AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; DISULFIDE BOND; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME STABILITY; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; HALF LIFE TIME; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; THERMOSTABILITY;

BACTERIA (MICROORGANISMS); PSEUDOMONAS; PSEUDOMONAS SP.; PSEUDOMONAS SP. KFCC10818;

EID: 0030004687     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.1996.0647     Document Type: Article

References (14)

1. Jang, W. H., Kim, E. K., Lee, H. H. B., Chung, J. H., and Yoo, O. J. (1996) Biotech Lett. 18, 57-62.
2. Wells, J. A., and Powers, D. B. (1986) J. Biol. Chem. 261, 6574-6570.
3. Pantoliano, M. W., Lander, R. C., Bryan, P. N., Rollence, M. L., Wood, J. F., and Poulos, T. L. (1987) Biochemistry 26, 2077-2082.
4. Takagi, H., Takahashi, T., Momose, H., Inouye, M., Maeda, Y., Matsuzawa, H., and Ohta, T. (1990) J. Biol. Chem. 265, 6874-6878.
5. Matsuzawa, H., Tokugawa, H., Hamaoki, M., Mizoguchi, M., Taguchi, H., Terada, I., Kwon, S,-T., and Ohta, T. (1988) Eur. J. Biochem. 171, 441-447.
6. Kwon, S,-T., Matsuzawa, H., and Ohta, T. (1988) J. Biochem. (Tokyo) 104, 557-559.
7. Kunkel, T. A. (1985) Proc. Natl. Acad. Sci. U.S.A. 82, 488-492.
8. Laemmli, U. K. (1970) Nature 227, 680-685.
9. Wells, J. A., Ferrari, F., Henneer, D. J., Estell, D. A., and Chen, E. Y. (1983) Nucleic Acids Res. 11, 7911-7925.
10. Stahl, M. L., and Ferrari, E. (1984) J. Bacteriol. 158, 411-418.
11. Kwon, S.-T., Terada, I., Matsuzawa, H., and Ohta, T. (1988) Eur. J. Biochem. 173, 491-497.
12. Stryer, L. (1988) Biochemistry pp. 187-191, Freeman, New York.
13. Bae, K. H., Jang, J. S., Park, K. S., Lee, S. H., and Byun, S. M. (1995) Biochem. Biophys. Res. Commun. 207, 20-24.
14. Jang, J. S., Park, D. K., Chun, M. J., and Byun, S. M. (1992) Biochim. Biophys. Acta 1162, 233-235.