SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract

Cell

Volumn 182, Issue 2, 2020, Pages 429-446.e14

  Hou, Yixuan J ^a   Okuda, Kenichi ^b   Edwards, Caitlin E ^a   Martinez, David R ^a   Asakura, Takanori ^b   Dinnon, Kenneth H ^b   Kato, Takafumi ^b   Lee, Rhianna E ^b   Yount, Boyd L ^a   Mascenik, Teresa M ^b   Chen, Gang ^b   Olivier, Kenneth N ⁱ   Ghio, Andrew ^j   Tse, Longping V ^a   Leist, Sarah R ^a   Gralinski, Lisa E ^a   Schäfer, Alexandra ^a   Dang, Hong ^b   Gilmore, Rodney ^b   Nakano, Satoko ^b   Sun, Ling ^b   Fulcher, M Leslie ^b   Livraghi Butrico, Alessandra ^b   Nicely, Nathan I ^c   Cameron, Mark ^f   Cameron, Cheryl ^f   Kelvin, David J ^e,k   de Silva, Aravinda ^b   Margolis, David M ^b,c   Markmann, Alena ^b   Bartelt, Luther ^b   Zumwalt, Ross ^g   Martinez, Fernando J ^h   Salvatore, Steven P ^h   Borczuk, Alain ^h   Tata, Purushothama R ^d   Sontake, Vishwaraj ^d   Kimple, Adam ^b   Jaspers, Ilona ^b   O'Neal, Wanda K ^b   Randell, Scott H ^b   Boucher, Richard C ^b   Baric, Ralph S ^a,b   more..

^a University of North Carolina at Chapel Hill   (United States)

^b UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF NORTH CAROLINA   (United States)

^d DUKE UNIVERSITY MEDICAL CENTER   (United States)

^e DALHOUSIE UNIVERSITY   (Canada)

^f CASE WESTERN RESERVE UNIVERSITY   (United States)

^g UNIVERSITY OF NEW MEXICO SCHOOL OF MEDICINE   (United States)

^h WEILL CORNELL MEDICAL COLLEGE   (United States)

ⁱ NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

^j U S ENVIRONMENTAL PROTECTION AGENCY   (United States)

^k SHANTOU UNIVERSITY MEDICAL COLLEGE   (China)

more..

Author keywords

ACE2; COVID 19; infectious clone; nasal infection; neutralization assay; primary cells; reporter virus; respiratory tropism; reverse genetics; SARS CoV 2

Indexed keywords

ANGIOTENSIN CONVERTING ENZYME 2; DIPEPTIDYL CARBOXYPEPTIDASE; FURIN; MONOCLONAL ANTIBODY; NEUTRALIZING ANTIBODY; RECOMBINANT DNA; SERINE PROTEINASE; TMPRSS2 PROTEIN, HUMAN;

ADULT; AGED; AIRWAY; ANIMAL CELL; ARTICLE; AUTOPSY; CONTROLLED STUDY; CORONAVIRUS DISEASE 2019; FEMALE; HUMAN; HUMAN CELL; HUMAN TISSUE; LOWER RESPIRATORY TRACT; LUNG ALVEOLUS; LUNG ALVEOLUS CELL TYPE 2; LUNG EPITHELIUM; MALE; NONHUMAN; NOSE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RESPIRATORY SYSTEM; REVERSE GENETICS; RNA ANALYSIS; SEVERE ACUTE RESPIRATORY SYNDROME; SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS 2; VERY ELDERLY; VIRUS NEUTRALIZATION; VIRUS PATHOGENESIS; ANIMAL; BETACORONAVIRUS; CELL CULTURE; CELL LINE; CHLOROCEBUS AETHIOPS; CORONAVIRUS INFECTION; CYSTIC FIBROSIS; GENETICS; IMMUNOLOGY; LUNG; METABOLISM; MIDDLE AGED; NOSE MUCOSA; PANDEMIC; PASSIVE IMMUNIZATION; PATHOGENICITY; PATHOLOGY; PROCEDURES; VERO CELL LINE; VIROLOGY; VIRULENCE; VIRUS PNEUMONIA; VIRUS REPLICATION;

AGED; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODIES, NEUTRALIZING; BETACORONAVIRUS; CELL LINE; CELLS, CULTURED; CHLOROCEBUS AETHIOPS; CORONAVIRUS INFECTIONS; CYSTIC FIBROSIS; DNA, RECOMBINANT; FEMALE; FURIN; HUMANS; IMMUNIZATION, PASSIVE; LUNG; MALE; MIDDLE AGED; NASAL MUCOSA; PANDEMICS; PEPTIDYL-DIPEPTIDASE A; PNEUMONIA, VIRAL; RESPIRATORY SYSTEM; REVERSE GENETICS; SERINE ENDOPEPTIDASES; VERO CELLS; VIRULENCE; VIRUS REPLICATION;

EID: 85086399821     PISSN: 00928674     EISSN: 10974172     Source Type: Journal    
DOI: 10.1016/j.cell.2020.05.042     Document Type: Article

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