A spike-modified Middle East respiratory syndrome coronavirus (MERS-CoV) infectious clone elicits mild respiratory disease in infected rhesus macaques

Scientific Reports

Volumn 8, Issue 1, 2018, Pages

  Cockrell, Adam S ^a   Johnson, Joshua C ^b   Moore, Ian N ^b   Liu, David X ^b   Bock, Kevin W ^b   Douglas, Madeline G ^a   Graham, Rachel L ^a   Solomon, Jeffrey ^c   Torzewski, Lisa ^b   Bartos, Christopher ^b   Hart, Randy ^b   Baric, Ralph S ^a   Johnson, Reed F ^d  

^a University of North Carolina at Chapel Hill   (United States)

^b NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^c FREDERICK NATIONAL LABORATORY FOR CANCER RESEARCH   (United States)

^d Emerging Viral Pathogens Section   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS RNA;

ANIMAL; CORONAVIRUS INFECTION; DIAGNOSTIC IMAGING; DISEASE MODEL; GENETICS; HUMAN; IMAGE PROCESSING; ISOLATION AND PURIFICATION; LUNG; MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS; PATHOGENICITY; PATHOLOGY; PHYSIOLOGY; RHESUS MONKEY; SEVERITY OF ILLNESS INDEX; VIROLOGY; VIRUS LOAD; VIRUS REPLICATION; X-RAY COMPUTED TOMOGRAPHY;

ANIMALS; CORONAVIRUS INFECTIONS; DISEASE MODELS, ANIMAL; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; LUNG; MACACA MULATTA; MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS; RNA, VIRAL; SEVERITY OF ILLNESS INDEX; TOMOGRAPHY, X-RAY COMPUTED; VIRAL LOAD; VIRUS REPLICATION;

EID: 85050408837     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/s41598-018-28900-1     Document Type: Article

References (23)

1. Arabi, Y. M. et al. Middle East Respiratory Syndrome. The New England journal of medicine 376, 584-594, https://doi.org/10.1056/NEJMsr1408795 (2017).
2. Cockrell, A. S. et al. A mouse model for MERS coronavirus-induced acute respiratory distress syndrome. Nature microbiology 2, 16226, https://doi.org/10.1038/nmicrobiol.2016.226 (2016).
3. Li, K. et al. Mouse-Adapted MERS coronavirus causes lethal lung disease in human DPP4 knockin mice. Proceedings of the National Academy of Sciences of the United States of America 114, E3119-E3128, https://doi.org/10.1073/pnas.1619109114 (2017).
4. Kim, S. Y. et al. Viral RNA in Blood as Indicator of Severe Outcome in Middle East Respiratory Syndrome Coronavirus Infection. Emerg Infect Dis 22, 1813-1816, https://doi.org/10.3201/eid2210.160218 (2016).
5. Oh, M. D. et al. Viral Load Kinetics of MERS Coronavirus Infection. The New England journal of medicine 375, 1303-1305, https://doi.org/10.1056/NEJMc1511695 (2016).
6. Zhao, J. et al. Recovery from the Middle East respiratory syndrome is associated with antibody and T-cell responses. Sci Immunol 2, https://doi.org/10.1126/sciimmunol.aan5393 (2017).
7. Ng, D. L. et al. Clinicopathologic, Immunohistochemical, and Ultrastructural Findings of a Fatal Case of Middle East Respiratory Syndrome Coronavirus Infection in the United Arab Emirates, April 2014. Am J Pathol 186, 652-658, https://doi.org/10.1016/j.ajpath.2015.10.024 (2016).
8. Alsaad, K. O. et al. Histopathology of Middle East respiratory syndrome coronovirus (MERS-CoV) infection-clinicopathological and ultrastructural study. Histopathology. https://doi.org/10.1111/his.13379 (2017).
9. Chan, J. F. et al. Treatment With Lopinavir/Ritonavir or Interferon-beta1b Improves Outcome of MERS-CoV Infection in a Nonhuman Primate Model of Common Marmoset. J Infect Dis 212, 1904-1913, https://doi.org/10.1093/infdis/jiv392 (2015).
10. Johnson, R. F. et al. 3B11-N, a monoclonal antibody against MERS-CoV, reduces lung pathology in rhesus monkeys following intratracheal inoculation of MERS-CoV Jordan-n3/2012. Virology 490, 49-58, https://doi.org/10.1016/j.virol.2016.01.004 (2016).
11. Johnson, R. F. et al. Intratracheal exposure of common marmosets to MERS-CoV Jordan-n3/2012 or MERS-CoV EMC/2012 isolates does not result in lethal disease. Virology 485, 422-430, https://doi.org/10.1016/j.virol.2015.07.013 (2015).
12. Munster, V. J., de Wit, E. & Feldmann, H. Pneumonia from human coronavirus in a macaque model. The New England journal of medicine 368, 1560-1562, https://doi.org/10.1056/NEJMc1215691 (2013).
13. Yao, Y. et al. An animal model of MERS produced by infection of rhesus macaques with MERS coronavirus. J Infect Dis 209, 236-242, https://doi.org/10.1093/infdis/jit590 (2014).
14. de Wit, E. et al. Middle East respiratory syndrome coronavirus (MERS-CoV) causes transient lower respiratory tract infection in rhesus macaques. Proceedings of the National Academy of Sciences of the United States of America 110, 16598-16603, https://doi.org/10.1073/pnas.1310744110 (2013).
15. Falzarano, D. et al. Infection with MERS-CoV causes lethal pneumonia in the common marmoset. PLoS Pathog 10, e1004250, https://doi.org/10.1371/journal.ppat.1004250 (2014).
16. de Wit, E., van Doremalen, N., Falzarano, D. & Munster, V. J. SARS and MERS: recent insights into emerging coronaviruses. Nat Rev Microbiol 14, 523-534, https://doi.org/10.1038/nrmicro.2016.81 (2016).
17. van Doremalen, N. et al. Efficacy of antibody-based therapies against Middle East respiratory syndrome coronavirus (MERS-CoV) in common marmosets. Antiviral research 143, 30-37, https://doi.org/10.1016/j.antiviral.2017.03.025 (2017).
18. Solomon, J., Douglas, D., Johnson, R. & Hammoud, D. In 2014 IEEE 27th International Symposium on Computer-Based Medical Systems. 169-172 (2014).
19. Johnson, R. F. et al. Small particle aerosol inoculation of cowpox Brighton Red in rhesus monkeys results in a severe respiratory disease. Virology 481, 124-135, https://doi.org/10.1016/j.virol.2015.02.044 (2015).
20. Johnson, R. F. et al. Exposure of rhesus monkeys to cowpox virus Brighton Red by large-particle aerosol droplets results in an upper respiratory tract disease. The Journal of general virology 97, 1942-1954, https://doi.org/10.1099/jgv.0.000501 (2016).
21. Meyerholz, D. K., Lambertz, A. M. & McCray, P. B. Jr. Dipeptidyl Peptidase 4 Distribution in the Human Respiratory Tract: Implications for the Middle East Respiratory Syndrome. Am J Pathol 186, 78-86, https://doi.org/10.1016/j.ajpath.2015.09.014 (2016).
22. van der Velden, V. H. et al. Expression of aminopeptidase N and dipeptidyl peptidase IV in the healthy and asthmatic bronchus. Clinical and experimental allergy: journal of the British Society for Allergy and Clinical Immunology 28, 110-120 (1998).
23. Hatziioannou, T. et al. HIV-1-induced AIDS in monkeys. Science (New York, N.Y.) 344, 1401-1405, https://doi.org/10.1126/science.1250761 (2014).