Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform

Nature

Volumn 582, Issue 7813, 2020, Pages 561-565

  Thi Nhu Thao, Tran ^a,b   Labroussaa, Fabien ^b   Ebert, Nadine ^a,b   V’kovski, Philip ^a,b   Stalder, Hanspeter ^a,b   Portmann, Jasmine ^a,b   Kelly, Jenna ^a,b   Steiner, Silvio ^a,b   Holwerda, Melle ^a,b   Kratzel, Annika ^a,b   Gultom, Mitra ^a,b   Schmied, Kimberly ^a,b   Laloli, Laura ^a,b   Hüsser, Linda ^a,b   Wider, Manon ^b   Pfaender, Stephanie ^a,b,c   Hirt, Dagny ^a,b   Cippà, Valentina ^b   Crespo Pomar, Silvia ^b   Schröder, Simon ^d   Muth, Doreen ^d,e   Niemeyer, Daniela ^d,e   Corman, Victor M ^d,e   Müller, Marcel A ^d,e,f   Drosten, Christian ^d,e   Dijkman, Ronald ^a,b   Jores, Joerg ^b   Thiel, Volker ^a,b   more..

^a INSTITUTE OF VIROLOGY AND IMMUNOLOGY   (Switzerland)

^b UNIVERSITY OF BERN   (Switzerland)

^c RUHR UNIVERSITY BOCHUM   (Germany)

^d BERLIN INSTITUTE OF HEALTH   (Germany)

^e CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^f I M SECHENOV FIRST MOSCOW STATE MEDICAL UNIVERSITY   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; RNA POLYMERASE; VIRUS DNA; BACTERIOPHAGE T7 RNA POLYMERASE; DNA DIRECTED RNA POLYMERASE; VIRAL PROTEIN;

CLONE; DISEASE TREATMENT; DRUG DEVELOPMENT; GENETIC ENGINEERING; GENOMICS; RECOMBINATION; VACCINE; VIRAL DISEASE; YEAST;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CORONAVIRIDAE; CORONAVIRUS DISEASE 2019; FLAVIVIRIDAE; GENE SYNTHESIS; GENETIC ENGINEERING; GENOMIC FRAGMENT; HUMAN; HUMAN CELL; HUMAN TISSUE; MOUSE; NONHUMAN; PANDEMIC; PNEUMOVIRINAE; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS 2; VIRAL GENETICS; VIRUS ISOLATION; YEAST ARTIFICIAL CHROMOSOME; ANIMAL; BETACORONAVIRUS; CHINA; CHLOROCEBUS AETHIOPS; CORONAVIRUS INFECTION; GENETICS; GENOMICS; METABOLISM; MOLECULAR CLONING; MOLECULAR EVOLUTION; MUTATION; PNEUMOVIRUS; PROCEDURES; REVERSE GENETICS; SYNTHETIC BIOLOGY; VERO CELL LINE; VIROLOGY; VIRUS GENOME; VIRUS PNEUMONIA; ZIKA VIRUS;

CORONAVIRIDAE; CORONAVIRUS; ESCHERICHIA COLI; FLAVIVIRIDAE; RNA VIRUSES; SACCHAROMYCES CEREVISIAE; SARS CORONAVIRUS;

ANIMALS; BETACORONAVIRUS; CHINA; CHLOROCEBUS AETHIOPS; CHROMOSOMES, ARTIFICIAL, YEAST; CLONING, MOLECULAR; CORONAVIRUS INFECTIONS; DNA-DIRECTED RNA POLYMERASES; EVOLUTION, MOLECULAR; GENOME, VIRAL; GENOMICS; HUMANS; MUTATION; PANDEMICS; PNEUMONIA, VIRAL; RESPIRATORY SYNCYTIAL VIRUSES; REVERSE GENETICS; SACCHAROMYCES CEREVISIAE; SYNTHETIC BIOLOGY; VERO CELLS; VIRAL PROTEINS; ZIKA VIRUS;

EID: 85084446510     PISSN: 00280836     EISSN: 14764687     Source Type: Journal    
DOI: 10.1038/s41586-020-2294-9     Document Type: Article

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