Enhanced Biosynthesis Performance of Heterologous Proteins in CHO-K1 Cells Using CRISPR-Cas9

ACS Synthetic Biology

Volumn 7, Issue 5, 2018, Pages 1259-1268

  Wang, Wenpeng ^a   Zheng, Wenyun ^b   Hu, Fengzhi ^b   He, Xiujuan ^b   Wu, Dong ^a   Zhang, Wenliang ^c   Liu, Haipeng ^d   Ma, Xingyuan ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^c UNIVERSITY OF NORTH CAROLINA AT GREENSBORO   (United States)

^d XIAMEN UNIVERSITY   (China)

Author keywords

antiapoptotic ability; CHO K1 mammalian cell; ER microenvironment; genome editing by CRISPR Cas9; high quality protein production

Indexed keywords

GENOMIC DNA; GLUTATHIONE; GLUTATHIONE DISULFIDE; RECOMBINANT PROTEIN; SURVIVIN; BIRC5 PROTEIN, HUMAN; GREEN FLUORESCENT PROTEIN; LUCIFERASE; OXIDOREDUCTASE; QSOX1 PROTEIN, HUMAN; STAUROSPORINE;

AMPLICON; ANIMAL CELL; APOPTOSIS; ARTICLE; CELL VIABILITY; CHINESE HAMSTER; CHO-K1 CELL LINE; CONFOCAL LASER SCANNING MICROSCOPY; CONTROLLED STUDY; CRISPR-CAS9 SYSTEM; DNA END JOINING REPAIR; ENDOPLASMIC RETICULUM; EXON; FLOW CYTOMETRY; GENE DELETION; GENE EDITING; GENE LOCUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; ANIMAL; CHO CELL LINE; CRICETULUS; CRISPR CAS SYSTEM; DRUG EFFECT; GENETICS; METABOLISM; PLASMID; PROCEDURES; PROTEIN ENGINEERING;

ANIMALS; APOPTOSIS; CHO CELLS; CRICETULUS; CRISPR-CAS SYSTEMS; DNA END-JOINING REPAIR; ENDOPLASMIC RETICULUM; GLUTATHIONE; GREEN FLUORESCENT PROTEINS; LUCIFERASES; OXIDOREDUCTASES ACTING ON SULFUR GROUP DONORS; PLASMIDS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; STAUROSPORINE; SURVIVIN;

EID: 85046435159     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/acssynbio.7b00375     Document Type: Article

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