Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair

Nucleic Acids Research

Volumn 44, Issue 9, 2016, Pages

  He, Xiangjun ^a   Tan, Chunlai ^a   Wang, Feng ^a   Wang, Yaofeng ^a   Zhou, Rui ^a   Cui, Dexuan ^a   You, Wenxing ^a   Zhao, Hui ^a   Ren, Jianwei ^a,b   Feng, Bo ^a  

^a CHINESE UNIVERSITY OF HONG KONG   (Hong Kong)

^b and Hong Kong Branch of CAS Center for Excellence in Animal Evolution and Genetics   (Hong Kong)

Author keywords

[No Author keywords available]

Indexed keywords

CELL PROTEIN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GREEN FLUORESCENT PROTEIN; PROTEIN CAS9; UNCLASSIFIED DRUG; DNA; GUIDE RNA;

ARTICLE; CELL SPECIFICITY; CONTROLLED STUDY; CRISPR CAS SYSTEM; DNA END JOINING REPAIR; DNA INTEGRATION; DNA REPAIR; DOUBLE STRANDED DNA BREAK; EMBRYO; EMBRYONIC STEM CELL; GENE LOCUS; GENE TARGETING; GENETIC TRANSFECTION; GENOME ANALYSIS; GENOME SIZE; HOMOLOGY DIRECTED REPAIR; HUMAN; HUMAN CELL; PLASMID; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REPORTER GENE; RNA EDITING; SEQUENCE HOMOLOGY; SOMATIC CELL; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CYTOLOGY; GENE EDITING; GENETICS; HCT 116 CELL LINE; HEK293 CELL LINE; HUMAN EMBRYONIC STEM CELL; PROCEDURES; RECOMBINATION REPAIR; TUMOR CELL LINE;

CELL LINE, TUMOR; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-CAS SYSTEMS; DNA; DNA BREAKS, DOUBLE-STRANDED; DNA END-JOINING REPAIR; GENE EDITING; GENE KNOCK-IN TECHNIQUES; GENES, REPORTER; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (PHOSPHORYLATING); GREEN FLUORESCENT PROTEINS; HCT116 CELLS; HEK293 CELLS; HUMAN EMBRYONIC STEM CELLS; HUMANS; RECOMBINATIONAL DNA REPAIR; RNA, GUIDE;

EID: 84969963103     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkw064     Document Type: Article

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