CRISPR-Cas targeted plasmid integration into mammalian cells via non-homologous end joining

Biotechnology and Bioengineering

Volumn 112, Issue 10, 2015, Pages 2154-2162

  Bachu, Ravichandra ^a   Bergareche, Iñigo ^a,b   Chasin, Lawrence A ^a  

^a Howard Hughes Medical Institute   (United States)

^b The Livelindly Company   (United States)

Author keywords

CHO; CRISPR Cas; HEK293; NHEJ; Site directed insertion

Indexed keywords

CELLS; DNA; GENE EXPRESSION; GENES; JOINING; MAMMALS; PROTEINS; RECOMBINANT PROTEINS;

CHO; CRISPR-CAS; HEK-293; NHEJ; SITE-DIRECTED INSERTION;

CYTOLOGY;

CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN; CRISPR ASSOCIATED PROTEIN; DNA DIRECTED RNA POLYMERASE III; ENDONUCLEASE; GENOMIC DNA; GUIDE RNA; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; PLASMID DNA; TIOGUANINE;

ANIMAL CELL; ARTICLE; CHO CELL LINE; CONTROLLED STUDY; CRISPR CAS SYSTEM; DNA CLEAVAGE; DNA END JOINING REPAIR; DNA SEQUENCE; DOUBLE STRANDED DNA BREAK; EXPRESSION VECTOR; FEMALE; GENE INSERTION; GENOME; HEK293 CELL LINE; HUMAN; HUMAN CELL; INVERSE POLYMERASE CHAIN REACTION; MAMMAL CELL; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; PLASMID; PROMOTER REGION; PROTEIN EXPRESSION; RESTRICTION SITE; SIMIAN VIRUS 40; SOUTHERN BLOTTING; STREPTOCOCCUS PYOGENES; ANIMAL; CELL LINE; GENETIC RECOMBINATION; MAMMAL; METABOLIC ENGINEERING; PROCEDURES;

MAMMALIA;

ANIMALS; CELL LINE; CRISPR-CAS SYSTEMS; DNA END-JOINING REPAIR; HUMANS; MAMMALS; METABOLIC ENGINEERING; PLASMIDS; RECOMBINATION, GENETIC;

EID: 84940467164     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25629     Document Type: Article

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