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Volumn 8, Issue , 2017, Pages 64-76

One-Step piggyBac Transposon-Based CRISPR/Cas9 Activation of Multiple Genes

Author keywords

CRISPR; gene activation; human induced pluripotent stem cells; piggyBac; transposon

Indexed keywords

BETA3 TUBULIN; BIOLOGICAL MARKER; LONG UNTRANSLATED RNA; MESSENGER RNA; MYT1 PROTEIN; NEUROGENIN 1; NEUROGENIN 2; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR FOXG1; TRANSCRIPTION FACTOR MNX1; TRANSCRIPTION FACTOR NFIA; TRANSCRIPTION FACTOR NFIB; TRANSCRIPTION FACTOR NFIX; TRANSCRIPTION FACTOR NKX2.2; TRANSCRIPTION FACTOR SOX10; TRANSCRIPTION FACTOR SOX11; TRANSCRIPTION FACTOR SOX2; TRANSCRIPTION FACTOR SOX3; TRANSCRIPTION FACTOR SOX8; TRANSCRIPTION FACTOR SOX9; UNCLASSIFIED DRUG;

EID: 85029305336     PISSN: None     EISSN: 21622531     Source Type: Journal    
DOI: 10.1016/j.omtn.2017.06.007     Document Type: Article
Times cited : (37)

References (64)
  • 1
    • 84865070369 scopus 로고    scopus 로고
    • A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity
    • Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J.A., Charpentier, E., A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337 (2012), 816–821.
    • (2012) Science , vol.337 , pp. 816-821
    • Jinek, M.1    Chylinski, K.2    Fonfara, I.3    Hauer, M.4    Doudna, J.A.5    Charpentier, E.6
  • 5
    • 84877707375 scopus 로고    scopus 로고
    • One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering
    • Wang, H., Yang, H., Shivalila, C.S., Dawlaty, M.M., Cheng, A.W., Zhang, F., Jaenisch, R., One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell 153 (2013), 910–918.
    • (2013) Cell , vol.153 , pp. 910-918
    • Wang, H.1    Yang, H.2    Shivalila, C.S.3    Dawlaty, M.M.4    Cheng, A.W.5    Zhang, F.6    Jaenisch, R.7
  • 6
    • 84884160273 scopus 로고    scopus 로고
    • CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering
    • Mali, P., Aach, J., Stranges, P.B., Esvelt, K.M., Moosburner, M., Kosuri, S., Yang, L., Church, G.M., CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat. Biotechnol. 31 (2013), 833–838.
    • (2013) Nat. Biotechnol. , vol.31 , pp. 833-838
    • Mali, P.1    Aach, J.2    Stranges, P.B.3    Esvelt, K.M.4    Moosburner, M.5    Kosuri, S.6    Yang, L.7    Church, G.M.8
  • 7
    • 84860440653 scopus 로고    scopus 로고
    • RNA in defense: CRISPRs protect prokaryotes against mobile genetic elements
    • Jore, M.M., Brouns, S.J., van der Oost, J., RNA in defense: CRISPRs protect prokaryotes against mobile genetic elements. Cold Spring Harb. Perspect. Biol., 4, 2012, a003657.
    • (2012) Cold Spring Harb. Perspect. Biol. , vol.4 , pp. a003657
    • Jore, M.M.1    Brouns, S.J.2    van der Oost, J.3
  • 8
    • 84866859751 scopus 로고    scopus 로고
    • Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria
    • Gasiunas, G., Barrangou, R., Horvath, P., Siksnys, V., Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria. Proc. Natl. Acad. Sci. USA 109 (2012), E2579–E2586.
    • (2012) Proc. Natl. Acad. Sci. USA , vol.109 , pp. E2579-E2586
    • Gasiunas, G.1    Barrangou, R.2    Horvath, P.3    Siksnys, V.4
  • 13
    • 84874687019 scopus 로고    scopus 로고
    • Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression
    • Qi, L.S., Larson, M.H., Gilbert, L.A., Doudna, J.A., Weissman, J.S., Arkin, A.P., Lim, W.A., Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell 152 (2013), 1173–1183.
    • (2013) Cell , vol.152 , pp. 1173-1183
    • Qi, L.S.1    Larson, M.H.2    Gilbert, L.A.3    Doudna, J.A.4    Weissman, J.S.5    Arkin, A.P.6    Lim, W.A.7
  • 15
    • 84908328232 scopus 로고    scopus 로고
    • A protein-tagging system for signal amplification in gene expression and fluorescence imaging
    • Tanenbaum, M.E., Gilbert, L.A., Qi, L.S., Weissman, J.S., Vale, R.D., A protein-tagging system for signal amplification in gene expression and fluorescence imaging. Cell 159 (2014), 635–646.
    • (2014) Cell , vol.159 , pp. 635-646
    • Tanenbaum, M.E.1    Gilbert, L.A.2    Qi, L.S.3    Weissman, J.S.4    Vale, R.D.5
  • 18
    • 84940450173 scopus 로고    scopus 로고
    • piggyBac-ing models and new therapeutic strategies
    • Woodard, L.E., Wilson, M.H., piggyBac-ing models and new therapeutic strategies. Trends Biotechnol. 33 (2015), 525–533.
    • (2015) Trends Biotechnol. , vol.33 , pp. 525-533
    • Woodard, L.E.1    Wilson, M.H.2
  • 19
    • 0024449118 scopus 로고
    • Transposon mutagenesis of baculoviruses: analysis of Trichoplusia ni transposon IFP2 insertions within the FP-locus of nuclear polyhedrosis viruses
    • Cary, L.C., Goebel, M., Corsaro, B.G., Wang, H.G., Rosen, E., Fraser, M.J., Transposon mutagenesis of baculoviruses: analysis of Trichoplusia ni transposon IFP2 insertions within the FP-locus of nuclear polyhedrosis viruses. Virology 172 (1989), 156–169.
    • (1989) Virology , vol.172 , pp. 156-169
    • Cary, L.C.1    Goebel, M.2    Corsaro, B.G.3    Wang, H.G.4    Rosen, E.5    Fraser, M.J.6
  • 20
    • 23744479962 scopus 로고    scopus 로고
    • Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice
    • Ding, S., Wu, X., Li, G., Han, M., Zhuang, Y., Xu, T., Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice. Cell 122 (2005), 473–483.
    • (2005) Cell , vol.122 , pp. 473-483
    • Ding, S.1    Wu, X.2    Li, G.3    Han, M.4    Zhuang, Y.5    Xu, T.6
  • 21
    • 34347337601 scopus 로고    scopus 로고
    • Toward simpler and faster genome-wide mutagenesis in mice
    • Wu, S., Ying, G., Wu, Q., Capecchi, M.R., Toward simpler and faster genome-wide mutagenesis in mice. Nat. Genet. 39 (2007), 922–930.
    • (2007) Nat. Genet. , vol.39 , pp. 922-930
    • Wu, S.1    Ying, G.2    Wu, Q.3    Capecchi, M.R.4
  • 22
    • 85010635827 scopus 로고    scopus 로고
    • piggyBac mediates efficient in vivo CRISPR library screening for tumorigenesis in mice
    • Xu, C., Qi, X., Du, X., Zou, H., Gao, F., Feng, T., Lu, H., Li, S., An, X., Zhang, L., et al. piggyBac mediates efficient in vivo CRISPR library screening for tumorigenesis in mice. Proc. Natl. Acad. Sci. USA 114 (2017), 722–727.
    • (2017) Proc. Natl. Acad. Sci. USA , vol.114 , pp. 722-727
    • Xu, C.1    Qi, X.2    Du, X.3    Zou, H.4    Gao, F.5    Feng, T.6    Lu, H.7    Li, S.8    An, X.9    Zhang, L.10
  • 24
    • 84924219729 scopus 로고    scopus 로고
    • Efficient generation of myelinating oligodendrocytes from primary progressive multiple sclerosis patients by induced pluripotent stem cells
    • Douvaras, P., Wang, J., Zimmer, M., Hanchuk, S., O'Bara, M.A., Sadiq, S., Sim, F.J., Goldman, J., Fossati, V., Efficient generation of myelinating oligodendrocytes from primary progressive multiple sclerosis patients by induced pluripotent stem cells. Stem Cell Reports 3 (2014), 250–259.
    • (2014) Stem Cell Reports , vol.3 , pp. 250-259
    • Douvaras, P.1    Wang, J.2    Zimmer, M.3    Hanchuk, S.4    O'Bara, M.A.5    Sadiq, S.6    Sim, F.J.7    Goldman, J.8    Fossati, V.9
  • 25
    • 79955548121 scopus 로고    scopus 로고
    • OLIG gene targeting in human pluripotent stem cells for motor neuron and oligodendrocyte differentiation
    • Liu, Y., Jiang, P., Deng, W., OLIG gene targeting in human pluripotent stem cells for motor neuron and oligodendrocyte differentiation. Nat. Protoc. 6 (2011), 640–655.
    • (2011) Nat. Protoc. , vol.6 , pp. 640-655
    • Liu, Y.1    Jiang, P.2    Deng, W.3
  • 27
    • 0032033009 scopus 로고    scopus 로고
    • The bHLH protein NEUROGENIN 2 is a determination factor for epibranchial placode-derived sensory neurons
    • Fode, C., Gradwohl, G., Morin, X., Dierich, A., LeMeur, M., Goridis, C., Guillemot, F., The bHLH protein NEUROGENIN 2 is a determination factor for epibranchial placode-derived sensory neurons. Neuron 20 (1998), 483–494.
    • (1998) Neuron , vol.20 , pp. 483-494
    • Fode, C.1    Gradwohl, G.2    Morin, X.3    Dierich, A.4    LeMeur, M.5    Goridis, C.6    Guillemot, F.7
  • 28
    • 0033967842 scopus 로고    scopus 로고
    • A role for neural determination genes in specifying the dorsoventral identity of telencephalic neurons
    • Fode, C., Ma, Q., Casarosa, S., Ang, S.L., Anderson, D.J., Guillemot, F., A role for neural determination genes in specifying the dorsoventral identity of telencephalic neurons. Genes Dev. 14 (2000), 67–80.
    • (2000) Genes Dev. , vol.14 , pp. 67-80
    • Fode, C.1    Ma, Q.2    Casarosa, S.3    Ang, S.L.4    Anderson, D.J.5    Guillemot, F.6
  • 29
    • 84859882919 scopus 로고    scopus 로고
    • Post-translational modification of Ngn2 differentially affects transcription of distinct targets to regulate the balance between progenitor maintenance and differentiation
    • Hindley, C., Ali, F., McDowell, G., Cheng, K., Jones, A., Guillemot, F., Philpott, A., Post-translational modification of Ngn2 differentially affects transcription of distinct targets to regulate the balance between progenitor maintenance and differentiation. Development 139 (2012), 1718–1723.
    • (2012) Development , vol.139 , pp. 1718-1723
    • Hindley, C.1    Ali, F.2    McDowell, G.3    Cheng, K.4    Jones, A.5    Guillemot, F.6    Philpott, A.7
  • 30
    • 12144271676 scopus 로고    scopus 로고
    • Olig2 and Ngn2 function in opposition to modulate gene expression in motor neuron progenitor cells
    • Lee, S.K., Lee, B., Ruiz, E.C., Pfaff, S.L., Olig2 and Ngn2 function in opposition to modulate gene expression in motor neuron progenitor cells. Genes Dev. 19 (2005), 282–294.
    • (2005) Genes Dev. , vol.19 , pp. 282-294
    • Lee, S.K.1    Lee, B.2    Ruiz, E.C.3    Pfaff, S.L.4
  • 31
    • 0032032016 scopus 로고    scopus 로고
    • neurogenin1 is essential for the determination of neuronal precursors for proximal cranial sensory ganglia
    • Ma, Q., Chen, Z., del Barco Barrantes, I., de la Pompa, J.L., Anderson, D.J., neurogenin1 is essential for the determination of neuronal precursors for proximal cranial sensory ganglia. Neuron 20 (1998), 469–482.
    • (1998) Neuron , vol.20 , pp. 469-482
    • Ma, Q.1    Chen, Z.2    del Barco Barrantes, I.3    de la Pompa, J.L.4    Anderson, D.J.5
  • 32
    • 0003073157 scopus 로고    scopus 로고
    • Identification of neurogenin, a vertebrate neuronal determination gene
    • Ma, Q., Kintner, C., Anderson, D.J., Identification of neurogenin, a vertebrate neuronal determination gene. Cell 87 (1996), 43–52.
    • (1996) Cell , vol.87 , pp. 43-52
    • Ma, Q.1    Kintner, C.2    Anderson, D.J.3
  • 34
    • 0035855786 scopus 로고    scopus 로고
    • Combinatorial roles of olig2 and neurogenin2 in the coordinated induction of pan-neuronal and subtype-specific properties of motoneurons
    • Mizuguchi, R., Sugimori, M., Takebayashi, H., Kosako, H., Nagao, M., Yoshida, S., Nabeshima, Y., Shimamura, K., Nakafuku, M., Combinatorial roles of olig2 and neurogenin2 in the coordinated induction of pan-neuronal and subtype-specific properties of motoneurons. Neuron 31 (2001), 757–771.
    • (2001) Neuron , vol.31 , pp. 757-771
    • Mizuguchi, R.1    Sugimori, M.2    Takebayashi, H.3    Kosako, H.4    Nagao, M.5    Yoshida, S.6    Nabeshima, Y.7    Shimamura, K.8    Nakafuku, M.9
  • 35
    • 0035855795 scopus 로고    scopus 로고
    • Coordinate regulation of motor neuron subtype identity and pan-neuronal properties by the bHLH repressor Olig2
    • Novitch, B.G., Chen, A.I., Jessell, T.M., Coordinate regulation of motor neuron subtype identity and pan-neuronal properties by the bHLH repressor Olig2. Neuron 31 (2001), 773–789.
    • (2001) Neuron , vol.31 , pp. 773-789
    • Novitch, B.G.1    Chen, A.I.2    Jessell, T.M.3
  • 36
    • 78651235357 scopus 로고    scopus 로고
    • Grafted human embryonic progenitors expressing neurogenin-2 stimulate axonal sprouting and improve motor recovery after severe spinal cord injury
    • Perrin, F.E., Boniface, G., Serguera, C., Lonjon, N., Serre, A., Prieto, M., Mallet, J., Privat, A., Grafted human embryonic progenitors expressing neurogenin-2 stimulate axonal sprouting and improve motor recovery after severe spinal cord injury. PLoS ONE, 5, 2010, e15914.
    • (2010) PLoS ONE , vol.5 , pp. e15914
    • Perrin, F.E.1    Boniface, G.2    Serguera, C.3    Lonjon, N.4    Serre, A.5    Prieto, M.6    Mallet, J.7    Privat, A.8
  • 37
    • 58449121331 scopus 로고    scopus 로고
    • Involvement of Ngn2, Tbr and NeuroD proteins during postnatal olfactory bulb neurogenesis
    • Roybon, L., Deierborg, T., Brundin, P., Li, J.Y., Involvement of Ngn2, Tbr and NeuroD proteins during postnatal olfactory bulb neurogenesis. Eur. J. Neurosci. 29 (2009), 232–243.
    • (2009) Eur. J. Neurosci. , vol.29 , pp. 232-243
    • Roybon, L.1    Deierborg, T.2    Brundin, P.3    Li, J.Y.4
  • 38
    • 62849111110 scopus 로고    scopus 로고
    • Neurogenin2 directs granule neuroblast production and amplification while NeuroD1 specifies neuronal fate during hippocampal neurogenesis
    • Roybon, L., Hjalt, T., Stott, S., Guillemot, F., Li, J.Y., Brundin, P., Neurogenin2 directs granule neuroblast production and amplification while NeuroD1 specifies neuronal fate during hippocampal neurogenesis. PLoS ONE, 4, 2009, e4779.
    • (2009) PLoS ONE , vol.4 , pp. e4779
    • Roybon, L.1    Hjalt, T.2    Stott, S.3    Guillemot, F.4    Li, J.Y.5    Brundin, P.6
  • 39
    • 0035797449 scopus 로고    scopus 로고
    • Crossregulation between Neurogenin2 and pathways specifying neuronal identity in the spinal cord
    • Scardigli, R., Schuurmans, C., Gradwohl, G., Guillemot, F., Crossregulation between Neurogenin2 and pathways specifying neuronal identity in the spinal cord. Neuron 31 (2001), 203–217.
    • (2001) Neuron , vol.31 , pp. 203-217
    • Scardigli, R.1    Schuurmans, C.2    Gradwohl, G.3    Guillemot, F.4
  • 40
    • 0037062516 scopus 로고    scopus 로고
    • Transient expression of the bHLH factor neurogenin-2 marks a subpopulation of neural crest cells biased for a sensory but not a neuronal fate
    • Zirlinger, M., Lo, L., McMahon, J., McMahon, A.P., Anderson, D.J., Transient expression of the bHLH factor neurogenin-2 marks a subpopulation of neural crest cells biased for a sensory but not a neuronal fate. Proc. Natl. Acad. Sci. USA 99 (2002), 8084–8089.
    • (2002) Proc. Natl. Acad. Sci. USA , vol.99 , pp. 8084-8089
    • Zirlinger, M.1    Lo, L.2    McMahon, J.3    McMahon, A.P.4    Anderson, D.J.5
  • 43
    • 84901195977 scopus 로고    scopus 로고
    • Multiplexed and programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells
    • Nissim, L., Perli, S.D., Fridkin, A., Perez-Pinera, P., Lu, T.K., Multiplexed and programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells. Mol. Cell 54 (2014), 698–710.
    • (2014) Mol. Cell , vol.54 , pp. 698-710
    • Nissim, L.1    Perli, S.D.2    Fridkin, A.3    Perez-Pinera, P.4    Lu, T.K.5
  • 45
    • 84925262435 scopus 로고    scopus 로고
    • Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system
    • Xie, K., Minkenberg, B., Yang, Y., Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Proc. Natl. Acad. Sci. USA 112 (2015), 3570–3575.
    • (2015) Proc. Natl. Acad. Sci. USA , vol.112 , pp. 3570-3575
    • Xie, K.1    Minkenberg, B.2    Yang, Y.3
  • 46
    • 85005949914 scopus 로고    scopus 로고
    • Multiplex CRISPR/Cas9-based genome engineering enhanced by Drosha-mediated sgRNA-shRNA structure
    • Yan, Q., Xu, K., Xing, J., Zhang, T., Wang, X., Wei, Z., Ren, C., Liu, Z., Shao, S., Zhang, Z., Multiplex CRISPR/Cas9-based genome engineering enhanced by Drosha-mediated sgRNA-shRNA structure. Sci. Rep., 6, 2016, 38970.
    • (2016) Sci. Rep. , vol.6 , pp. 38970
    • Yan, Q.1    Xu, K.2    Xing, J.3    Zhang, T.4    Wang, X.5    Wei, Z.6    Ren, C.7    Liu, Z.8    Shao, S.9    Zhang, Z.10
  • 47
    • 84930812429 scopus 로고    scopus 로고
    • Direct cardiac reprogramming: progress and challenges in basic biology and clinical applications
    • Sadahiro, T., Yamanaka, S., Ieda, M., Direct cardiac reprogramming: progress and challenges in basic biology and clinical applications. Circ. Res. 116 (2015), 1378–1391.
    • (2015) Circ. Res. , vol.116 , pp. 1378-1391
    • Sadahiro, T.1    Yamanaka, S.2    Ieda, M.3
  • 48
    • 84981748049 scopus 로고    scopus 로고
    • Targeted Epigenetic Remodeling of Endogenous Loci by CRISPR/Cas9-Based Transcriptional Activators Directly Converts Fibroblasts to Neuronal Cells
    • Black, J.B., Adler, A.F., Wang, H.G., D'Ippolito, A.M., Hutchinson, H.A., Reddy, T.E., Pitt, G.S., Leong, K.W., Gersbach, C.A., Targeted Epigenetic Remodeling of Endogenous Loci by CRISPR/Cas9-Based Transcriptional Activators Directly Converts Fibroblasts to Neuronal Cells. Cell Stem Cell 19 (2016), 406–414.
    • (2016) Cell Stem Cell , vol.19 , pp. 406-414
    • Black, J.B.1    Adler, A.F.2    Wang, H.G.3    D'Ippolito, A.M.4    Hutchinson, H.A.5    Reddy, T.E.6    Pitt, G.S.7    Leong, K.W.8    Gersbach, C.A.9
  • 50
    • 84941047606 scopus 로고    scopus 로고
    • Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation
    • Balboa, D., Weltner, J., Eurola, S., Trokovic, R., Wartiovaara, K., Otonkoski, T., Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation. Stem Cell Reports 5 (2015), 448–459.
    • (2015) Stem Cell Reports , vol.5 , pp. 448-459
    • Balboa, D.1    Weltner, J.2    Eurola, S.3    Trokovic, R.4    Wartiovaara, K.5    Otonkoski, T.6
  • 51
    • 84955589837 scopus 로고    scopus 로고
    • Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators
    • Wei, S., Zou, Q., Lai, S., Zhang, Q., Li, L., Yan, Q., Zhou, X., Zhong, H., Lai, L., Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators. Sci. Rep., 6, 2016, 19648.
    • (2016) Sci. Rep. , vol.6 , pp. 19648
    • Wei, S.1    Zou, Q.2    Lai, S.3    Zhang, Q.4    Li, L.5    Yan, Q.6    Zhou, X.7    Zhong, H.8    Lai, L.9
  • 53
    • 73849086119 scopus 로고    scopus 로고
    • PiggyBac transposon-based inducible gene expression in vivo after somatic cell gene transfer
    • Saridey, S.K., Liu, L., Doherty, J.E., Kaja, A., Galvan, D.L., Fletcher, B.S., Wilson, M.H., PiggyBac transposon-based inducible gene expression in vivo after somatic cell gene transfer. Mol. Ther. 17 (2009), 2115–2120.
    • (2009) Mol. Ther. , vol.17 , pp. 2115-2120
    • Saridey, S.K.1    Liu, L.2    Doherty, J.E.3    Kaja, A.4    Galvan, D.L.5    Fletcher, B.S.6    Wilson, M.H.7
  • 55
    • 84904662494 scopus 로고    scopus 로고
    • Efficient germ-line transmission obtained with transgene-free induced pluripotent stem cells
    • Wu, S., Wu, Y., Zhang, X., Capecchi, M.R., Efficient germ-line transmission obtained with transgene-free induced pluripotent stem cells. Proc. Natl. Acad. Sci. USA 111 (2014), 10678–10683.
    • (2014) Proc. Natl. Acad. Sci. USA , vol.111 , pp. 10678-10683
    • Wu, S.1    Wu, Y.2    Zhang, X.3    Capecchi, M.R.4
  • 57
    • 85047687015 scopus 로고    scopus 로고
    • Seamless genome editing in human pluripotent stem cells using custom endonuclease-based gene targeting and the piggyBac transposon
    • Yusa, K., Seamless genome editing in human pluripotent stem cells using custom endonuclease-based gene targeting and the piggyBac transposon. Nat. Protoc. 8 (2013), 2061–2078.
    • (2013) Nat. Protoc. , vol.8 , pp. 2061-2078
    • Yusa, K.1
  • 58
    • 84875260104 scopus 로고    scopus 로고
    • The piggyBac transposon displays local and distant reintegration preferences and can cause mutations at noncanonical integration sites
    • Li, M.A., Pettitt, S.J., Eckert, S., Ning, Z., Rice, S., Cadiñanos, J., Yusa, K., Conte, N., Bradley, A., The piggyBac transposon displays local and distant reintegration preferences and can cause mutations at noncanonical integration sites. Mol. Cell. Biol. 33 (2013), 1317–1330.
    • (2013) Mol. Cell. Biol. , vol.33 , pp. 1317-1330
    • Li, M.A.1    Pettitt, S.J.2    Eckert, S.3    Ning, Z.4    Rice, S.5    Cadiñanos, J.6    Yusa, K.7    Conte, N.8    Bradley, A.9
  • 62
    • 84983121994 scopus 로고    scopus 로고
    • Human Induced Pluripotent Stem Cell NEUROG2 Dual Knockin Reporter Lines Generated by the CRISPR/Cas9 System
    • Li, S., Xue, H., Wu, J., Rao, M.S., Kim, D.H., Deng, W., Liu, Y., Human Induced Pluripotent Stem Cell NEUROG2 Dual Knockin Reporter Lines Generated by the CRISPR/Cas9 System. Stem Cells Dev. 24 (2015), 2925–2942.
    • (2015) Stem Cells Dev. , vol.24 , pp. 2925-2942
    • Li, S.1    Xue, H.2    Wu, J.3    Rao, M.S.4    Kim, D.H.5    Deng, W.6    Liu, Y.7
  • 63
    • 0035710746 scopus 로고    scopus 로고
    • Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method
    • Livak, K.J., Schmittgen, T.D., Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25 (2001), 402–408.
    • (2001) Methods , vol.25 , pp. 402-408
    • Livak, K.J.1    Schmittgen, T.D.2


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