Seamless genome editing in human pluripotent stem cells using custom endonuclease-based gene targeting and the piggyBac transposon

Nature Protocols

Volumn 8, Issue 10, 2013, Pages 2061-2078

  Yusa, Kosuke ^a  

^a WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ENDONUCLEASE; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR NUCLEASE; UNCLASSIFIED DRUG; ZINC FINGER NUCLEASE;

ARTICLE; CONTROLLED STUDY; DNA MODIFICATION; GENE TARGETING; GENE VECTOR; GENETIC MANIPULATION; GENETIC PROCEDURES; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; LOXP SITE; PIGGYBAC TRANSPOSON; PLURIPOTENT STEM CELL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEAMLESS GENOME EDITING; TRANSPOSON;

DNA TRANSPOSABLE ELEMENTS; GENE TARGETING; GENETIC ENGINEERING; GENETIC VECTORS; HUMANS; MUTATION; PLURIPOTENT STEM CELLS; TARGETED GENE REPAIR;

EID: 85047687015     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2013.126     Document Type: Article

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