메뉴 건너뛰기




Volumn 7, Issue 1, 2017, Pages

Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time

Author keywords

[No Author keywords available]

Indexed keywords

BETA ARRESTIN; LIGAND; LUCIFERASE; NANOPARTICLE; PROTEIN; PROTEIN BINDING;

EID: 85020633916     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/s41598-017-03486-2     Document Type: Article
Times cited : (45)

References (69)
  • 1
    • 84965017906 scopus 로고    scopus 로고
    • The beta-arrestins: Multifunctional regulators of g protein-coupled receptors
    • Smith, J. S. & Rajagopal, S. The beta-Arrestins: Multifunctional Regulators of G Protein-coupled Receptors. J Biol Chem 291, 8969-8977, doi:10.1074/jbc.R115.713313 (2016).
    • (2016) J Biol Chem , vol.291 , pp. 8969-8977
    • Smith, J.S.1    Rajagopal, S.2
  • 2
    • 84962459565 scopus 로고    scopus 로고
    • The conformational signature of beta-arrestin2 predicts its trafficking and signalling functions
    • Lee, M. H. et al. The conformational signature of beta-arrestin2 predicts its trafficking and signalling functions. Nature 531, 665-668, doi:10.1038/nature17154 (2016).
    • (2016) Nature , vol.531 , pp. 665-668
    • Lee, M.H.1
  • 3
    • 84962420929 scopus 로고    scopus 로고
    • Beta-Arrestin biosensors reveal a rapid, receptor-dependent activation/deactivation cycle
    • Nuber, S. et al. beta-Arrestin biosensors reveal a rapid, receptor-dependent activation/deactivation cycle. Nature 531, 661-664, doi:10.1038/nature17198 (2016).
    • (2016) Nature , vol.531 , pp. 661-664
    • Nuber, S.1
  • 4
    • 84865565200 scopus 로고    scopus 로고
    • Effect of enhanced Renilla luciferase and fluorescent protein variants on the Forster distance of Bioluminescence resonance energy transfer (BRET)
    • Dacres, H., Michie, M., Wang, J., Pfleger, K. D. & Trowell, S. C. Effect of enhanced Renilla luciferase and fluorescent protein variants on the Forster distance of Bioluminescence resonance energy transfer (BRET). Biochem Biophys Res Commun 425, 625-629, doi:10.1016/j.bbrc.2012.07.133 (2012).
    • (2012) Biochem Biophys Res Commun , vol.425 , pp. 625-629
    • Dacres, H.1    Michie, M.2    Wang, J.3    Pfleger, K.D.4    Trowell, S.C.5
  • 5
    • 33646343978 scopus 로고    scopus 로고
    • Illuminating insights into protein-protein interactions using bioluminescence resonance energy transfer (BRET)
    • Pfleger, K. D. & Eidne, K. A. Illuminating insights into protein-protein interactions using bioluminescence resonance energy transfer (BRET). Nat Methods 3, 165-174, doi:10.1038/nmeth841 (2006).
    • (2006) Nat Methods , vol.3 , pp. 165-174
    • Pfleger, K.D.1    Eidne, K.A.2
  • 6
    • 84858203893 scopus 로고    scopus 로고
    • Fluorescence/bioluminescence resonance energy transfer techniques to study G-proteincoupled receptor activation and signaling
    • Lohse, M. J., Nuber, S. & Hoffmann, C. Fluorescence/bioluminescence resonance energy transfer techniques to study G-proteincoupled receptor activation and signaling. Pharmacol Rev 64, 299-336, doi:10.1124/pr.110.004309 (2012).
    • (2012) Pharmacol Rev , vol.64 , pp. 299-336
    • Lohse, M.J.1    Nuber, S.2    Hoffmann, C.3
  • 7
    • 34250637175 scopus 로고    scopus 로고
    • Bioluminescence resonance energy transfer (BRET) for the real-time detection of protein-protein interactions
    • Pfleger, K. D., Seeber, R. M. & Eidne, K. A. Bioluminescence resonance energy transfer (BRET) for the real-time detection of protein-protein interactions. Nat Protoc 1, 337-345, doi:10.1038/nprot.2006.52 (2006).
    • (2006) Nat Protoc , vol.1 , pp. 337-345
    • Pfleger, K.D.1    Seeber, R.M.2    Eidne, K.A.3
  • 8
    • 84934434629 scopus 로고    scopus 로고
    • Application of BRET to monitor ligand binding to GPCRs
    • Stoddart, L. A. et al. Application of BRET to monitor ligand binding to GPCRs. Nat Methods 12, 661-663, doi:10.1038/nmeth.3398 (2015).
    • (2015) Nat Methods , vol.12 , pp. 661-663
    • Stoddart, L.A.1
  • 9
    • 84949294621 scopus 로고    scopus 로고
    • Target engagement and drug residence time can be observed in living cells with BRET
    • Robers, M. B. et al. Target engagement and drug residence time can be observed in living cells with BRET. Nature Communications 6, 10091, doi:10.1038/ncomms10091 (2015).
    • (2015) Nature Communications , vol.6 , pp. 10091
    • Robers, M.B.1
  • 10
    • 84881437733 scopus 로고    scopus 로고
    • The transience of transient overexpression
    • Gibson, T. J., Seiler, M. & Veitia, R. A. The transience of transient overexpression. Nat Methods 10, 715-721, doi:10.1038/nmeth.2534 (2013).
    • (2013) Nat Methods , vol.10 , pp. 715-721
    • Gibson, T.J.1    Seiler, M.2    Veitia, R.A.3
  • 11
    • 84908592499 scopus 로고    scopus 로고
    • Comparative assessment of fluorescent transgene methods for quantitative imaging in human cells
    • Mahen, R. et al. Comparative assessment of fluorescent transgene methods for quantitative imaging in human cells. Mol Biol Cell 25, 3610-3618, doi:10.1091/mbc.E14-06-1091 (2014).
    • (2014) Mol Biol Cell , vol.25 , pp. 3610-3618
    • Mahen, R.1
  • 12
    • 84939802598 scopus 로고    scopus 로고
    • Nanobret-A novel bret platform for the analysis of protein-protein interactions
    • Machleidt, T. et al. NanoBRET-A Novel BRET Platform for the Analysis of Protein-Protein Interactions. ACS Chem Biol 10, 1797-1804, doi:10.1021/acschembio.5b00143 (2015).
    • (2015) ACS Chem Biol , vol.10 , pp. 1797-1804
    • Machleidt, T.1
  • 13
    • 84876494666 scopus 로고    scopus 로고
    • A simple method to generate stable cell lines for the analysis of transient protein-protein interactions
    • Savage, E. E., Wootten, D., Christopoulos, A., Sexton, P. M. & Furness, S. G. A simple method to generate stable cell lines for the analysis of transient protein-protein interactions. Biotechniques 54, 217-221, doi:10.2144/000114013 (2013).
    • (2013) Biotechniques , vol.54 , pp. 217-221
    • Savage, E.E.1    Wootten, D.2    Christopoulos, A.3    Sexton, P.M.4    Furness, S.G.5
  • 14
    • 77955799768 scopus 로고    scopus 로고
    • Protein-protein interactions monitored in cells from transgenic mice using bioluminescence resonance energy transfer
    • Audet, M., Lagace, M., Silversides, D. W. & Bouvier, M. Protein-protein interactions monitored in cells from transgenic mice using bioluminescence resonance energy transfer. FASEB J 24, 2829-2838, doi:10.1096/fj.09-144816 (2010).
    • (2010) FASEB J , vol.24 , pp. 2829-2838
    • Audet, M.1    Lagace, M.2    Silversides, D.W.3    Bouvier, M.4
  • 15
    • 41149177031 scopus 로고    scopus 로고
    • Overview of genetic reporter systems
    • Unit9
    • Kain, S. R. & Ganguly, S. Overview of genetic reporter systems. Curr Protoc Mol Biol Chapter 9, Unit9 6, doi:10.1002/0471142727. mb0906s36 (2001).
    • (2001) Curr Protoc Mol Biol Chapter , vol.9 , pp. 6
    • Kain, S.R.1    Ganguly, S.2
  • 16
    • 84928974851 scopus 로고    scopus 로고
    • Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9
    • Rojas-Fernandez, A. et al. Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9. Sci Rep 5, 9811, doi:10.1038/srep09811 (2015).
    • (2015) Sci Rep , vol.5 , pp. 9811
    • Rojas-Fernandez, A.1
  • 17
    • 79960064013 scopus 로고    scopus 로고
    • Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting
    • Cermak, T. et al. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Res 39, e82, doi:10.1093/nar/gkr218 (2011).
    • (2011) Nucleic Acids Res , vol.39 , pp. e82
    • Cermak, T.1
  • 18
    • 84873729095 scopus 로고    scopus 로고
    • Multiplex genome engineering using CRISPR/Cas systems
    • Cong, L. et al. Multiplex genome engineering using CRISPR/Cas systems. Science 339, 819-823, doi:10.1126/science.1231143 (2013).
    • (2013) Science , vol.339 , pp. 819-823
    • Cong, L.1
  • 19
    • 84887010498 scopus 로고    scopus 로고
    • Genome engineering using the CRISPR-Cas9 system
    • Ran, F. A. et al. Genome engineering using the CRISPR-Cas9 system. Nat Protoc 8, 2281-2308, doi:10.1038/nprot.2013.143 (2013).
    • (2013) Nat Protoc , vol.8 , pp. 2281-2308
    • Ran, F.A.1
  • 20
    • 84944916655 scopus 로고    scopus 로고
    • Genome editing of CXCR4 by CRISPR/cas9 confers cells resistant to HIV-1 infection
    • Hou, P. et al. Genome editing of CXCR4 by CRISPR/cas9 confers cells resistant to HIV-1 infection. Sci Rep 5, 15577, doi:10.1038/ srep15577 (2015).
    • (2015) Sci Rep , vol.5 , pp. 15577
    • Hou, P.1
  • 21
    • 84940184252 scopus 로고    scopus 로고
    • Generation of knock-in primary human T cells using Cas9 ribonucleoproteins
    • Schumann, K. et al. Generation of knock-in primary human T cells using Cas9 ribonucleoproteins. Proc Natl Acad Sci U S A 112, 10437-10442, doi:10.1073/pnas.1512503112 (2015).
    • (2015) Proc Natl Acad Sci U S A , vol.112 , pp. 10437-10442
    • Schumann, K.1
  • 22
    • 84922671463 scopus 로고    scopus 로고
    • Efficient ablation of genes in human hematopoietic stem and effector cells using CRISPR/Cas9
    • Mandal, P. K. et al. Efficient ablation of genes in human hematopoietic stem and effector cells using CRISPR/Cas9. Cell Stem Cell 15, 643-652, doi:10.1016/j.stem.2014.10.004 (2014).
    • (2014) Cell Stem Cell , vol.15 , pp. 643-652
    • Mandal, P.K.1
  • 23
    • 84961654781 scopus 로고    scopus 로고
    • Versatile protein tagging in cells with split fluorescent protein
    • Kamiyama, D. et al. Versatile protein tagging in cells with split fluorescent protein. Nat Commun 7, 11046, doi:10.1038/ ncomms11046 (2016).
    • (2016) Nat Commun , vol.7 , pp. 11046
    • Kamiyama, D.1
  • 24
    • 84908328232 scopus 로고    scopus 로고
    • A protein-tagging system for signal amplification in gene expression and fluorescence imaging
    • Tanenbaum, M. E., Gilbert, L. A., Qi, L. S., Weissman, J. S. & Vale, R. D. A protein-tagging system for signal amplification in gene expression and fluorescence imaging. Cell 159, 635-646, doi:10.1016/j.cell.2014.09.039 (2014).
    • (2014) Cell , vol.159 , pp. 635-646
    • Tanenbaum, M.E.1    Gilbert, L.A.2    Qi, L.S.3    Weissman, J.S.4    Vale, R.D.5
  • 25
    • 85007602741 scopus 로고    scopus 로고
    • Targeted elimination of g proteins and arrestins defines their specific contributions to both intensity and duration of g protein-coupled receptor signalling
    • Alvarez-Curto, E. et al. Targeted Elimination of G proteins and Arrestins Defines their Specific Contributions to both Intensity and Duration of G protein-Coupled Receptor Signalling. J Biol Chem. doi:10.1074/jbc.M116.754887 (2016).
    • (2016) J Biol Chem
    • Alvarez-Curto, E.1
  • 26
    • 84928141099 scopus 로고    scopus 로고
    • CRISPR/Cas9-mediated endogenous protein tagging for RESOLFT super-resolution microscopy of living human cells
    • Ratz, M., Testa, I., Hell, S. W. & Jakobs, S. CRISPR/Cas9-mediated endogenous protein tagging for RESOLFT super-resolution microscopy of living human cells. Sci Rep 5, 9592, doi:10.1038/srep09592 (2015).
    • (2015) Sci Rep , vol.5 , pp. 9592
    • Ratz, M.1    Testa, I.2    Hell, S.W.3    Jakobs, S.4
  • 27
    • 84950283765 scopus 로고    scopus 로고
    • A generic strategy for CRISPR-Cas9-mediated gene tagging
    • Lackner, D. H. et al. A generic strategy for CRISPR-Cas9-mediated gene tagging. Nat Commun 6, 10237, doi:10.1038/ncomms10237 (2015).
    • (2015) Nat Commun , vol.6 , pp. 10237
    • Lackner, D.H.1
  • 28
    • 84884289608 scopus 로고    scopus 로고
    • One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering
    • Yang, H. et al. One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering. Cell 154, 1370-1379, doi:10.1016/j.cell.2013.08.022 (2013).
    • (2013) Cell , vol.154 , pp. 1370-1379
    • Yang, H.1
  • 29
    • 38149064653 scopus 로고    scopus 로고
    • Functional complementation of high-efficiency resonance energy transfer: A new tool for the study of protein binding interactions in living cells
    • Molinari, P., Casella, I. & Costa, T. Functional complementation of high-efficiency resonance energy transfer: a new tool for the study of protein binding interactions in living cells. Biochem J 409, 251-261, doi:10.1042/bj20070803 (2008).
    • (2008) Biochem J , vol.409 , pp. 251-261
    • Molinari, P.1    Casella, I.2    Costa, T.3
  • 30
    • 84869455585 scopus 로고    scopus 로고
    • Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate
    • Hall, M. P. et al. Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate. ACS Chem Biol 7, 1848-1857, doi:10.1021/cb3002478 (2012).
    • (2012) ACS Chem Biol , vol.7 , pp. 1848-1857
    • Hall, M.P.1
  • 31
    • 84974822953 scopus 로고    scopus 로고
    • Fast and high resolution single-cell BRET imaging
    • Goyet, E., Bouquier, N., Ollendorff, V. & Perroy, J. Fast and high resolution single-cell BRET imaging. Sci Rep 6, 28231, doi:10.1038/ srep28231 (2016).
    • (2016) Sci Rep , vol.6 , pp. 28231
    • Goyet, E.1    Bouquier, N.2    Ollendorff, V.3    Perroy, J.4
  • 32
    • 0032507962 scopus 로고    scopus 로고
    • Function of the chemokine receptor CXCR4 in haematopoiesis and in cerebellar development
    • Zou, Y. R., Kottmann, A. H., Kuroda, M., Taniuchi, I. & Littman, D. R. Function of the chemokine receptor CXCR4 in haematopoiesis and in cerebellar development. Nature 393, 595-599, doi:10.1038/31269 (1998).
    • (1998) Nature , vol.393 , pp. 595-599
    • Zou, Y.R.1    Kottmann, A.H.2    Kuroda, M.3    Taniuchi, I.4    Littman, D.R.5
  • 33
    • 84924943548 scopus 로고    scopus 로고
    • CXCR4 over-expression and survival in cancer: A system review and meta-analysis
    • Zhao, H. et al. CXCR4 over-expression and survival in cancer: a system review and meta-analysis. Oncotarget 6, 5022-5040, doi:10.18632/oncotarget.3217 (2015).
    • (2015) Oncotarget , vol.6 , pp. 5022-5040
    • Zhao, H.1
  • 34
    • 0037656291 scopus 로고    scopus 로고
    • Mutations in the chemokine receptor gene CXCR4 are associated with WHIM syndrome, a combined immunodeficiency disease
    • Hernandez, P. A. et al. Mutations in the chemokine receptor gene CXCR4 are associated with WHIM syndrome, a combined immunodeficiency disease. Nat Genet 34, 70-74, doi:10.1038/ng1149 (2003).
    • (2003) Nat Genet , vol.34 , pp. 70-74
    • Hernandez, P.A.1
  • 35
    • 0030002637 scopus 로고    scopus 로고
    • HIV-1 entry cofactor: Functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor
    • Feng, Y., Broder, C. C., Kennedy, P. E. & Berger, E. A. HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science 272, 872-877, doi:10.1126/science.272.5263.872 (1996).
    • (1996) Science , vol.272 , pp. 872-877
    • Feng, Y.1    Broder, C.C.2    Kennedy, P.E.3    Berger, E.A.4
  • 36
    • 77951230331 scopus 로고    scopus 로고
    • Site-specific phosphorylation of CXCR4 is dynamically regulated by multiple kinases and results in differential modulation of CXCR4 signaling
    • Busillo, J. M. et al. Site-specific phosphorylation of CXCR4 is dynamically regulated by multiple kinases and results in differential modulation of CXCR4 signaling. J Biol Chem 285, 7805-7817, doi:10.1074/jbc.M109.091173 (2010).
    • (2010) J Biol Chem , vol.285 , pp. 7805-7817
    • Busillo, J.M.1
  • 37
    • 0032530174 scopus 로고    scopus 로고
    • Loss of transcriptional activity of a transgene is accompanied by DNA methylation and histone deacetylation and is prevented by insulators
    • Pikaart, M. I., Recillas-Targa, F. & Felsenfeld, G. Loss of transcriptional activity of a transgene is accompanied by DNA methylation and histone deacetylation and is prevented by insulators. Genes & Development 12, 2852-2862, doi:10.1101/gad.12.18.2852 (1998).
    • (1998) Genes & Development , vol.12 , pp. 2852-2862
    • Pikaart, M.I.1    Recillas-Targa, F.2    Felsenfeld, G.3
  • 38
    • 0033562986 scopus 로고    scopus 로고
    • USF/c-Myc enhances, while Yin-Yang 1 suppresses, the promoter activity of CXCR4, a coreceptor for HIV-1 entry
    • Moriuchi, M., Moriuchi, H., Margolis, D. M. & Fauci, A. S. USF/c-Myc enhances, while Yin-Yang 1 suppresses, the promoter activity of CXCR4, a coreceptor for HIV-1 entry. J Immunol 162, 5986-5992 (1999).
    • (1999) J Immunol , vol.162 , pp. 5986-5992
    • Moriuchi, M.1    Moriuchi, H.2    Margolis, D.M.3    Fauci, A.S.4
  • 39
    • 84867290208 scopus 로고    scopus 로고
    • Measurements of beta-arrestin recruitment to activated seven transmembrane receptors using enzyme complementation
    • Bassoni, D. L., Raab, W. J., Achacoso, P. L., Loh, C. Y. & Wehrman, T. S. Measurements of beta-arrestin recruitment to activated seven transmembrane receptors using enzyme complementation. Methods Mol Biol 897, 181-203, doi:10.1007/978-1-61779-909-9-9 (2012).
    • (2012) Methods Mol Biol , vol.897 , pp. 181-203
    • Bassoni, D.L.1    Raab, W.J.2    Achacoso, P.L.3    Loh, C.Y.4    Wehrman, T.S.5
  • 40
    • 0037737763 scopus 로고    scopus 로고
    • Trafficking patterns of beta-arrestin and G protein-coupled receptors determined by the kinetics of beta-arrestin deubiquitination
    • Shenoy, S. K. & Lefkowitz, R. J. Trafficking patterns of beta-arrestin and G protein-coupled receptors determined by the kinetics of beta-arrestin deubiquitination. J Biol Chem 278, 14498-14506, doi:10.1074/jbc.M209626200 (2003).
    • (2003) J Biol Chem , vol.278 , pp. 14498-14506
    • Shenoy, S.K.1    Lefkowitz, R.J.2
  • 41
    • 79952046779 scopus 로고    scopus 로고
    • Internalization dissociates beta2-adrenergic receptors
    • Lan, T. H., Kuravi, S. & Lambert, N. A. Internalization dissociates beta2-adrenergic receptors. PLoS One 6, e17361, doi:10.1371/ journal.pone.0017361 (2011).
    • (2011) PLoS One , vol.6 , pp. e17361
    • Lan, T.H.1    Kuravi, S.2    Lambert, N.A.3
  • 42
    • 84980417178 scopus 로고    scopus 로고
    • Mutations of vasopressin receptor 2 including novel l312s have differential effects on trafficking
    • Tiulpakov, A. et al. Mutations of Vasopressin Receptor 2 Including Novel L312S Have Differential Effects on Trafficking. Mol Endocrinol 30, 889-904, doi:10.1210/me.2016-1002 (2016).
    • (2016) Mol Endocrinol , vol.30 , pp. 889-904
    • Tiulpakov, A.1
  • 43
    • 19944428599 scopus 로고    scopus 로고
    • Incorporation of CXCR4 into membrane lipid rafts primes homing-related responses of hematopoietic stem/ progenitor cells to an SDF-1 gradient
    • Wysoczynski, M. et al. Incorporation of CXCR4 into membrane lipid rafts primes homing-related responses of hematopoietic stem/ progenitor cells to an SDF-1 gradient. Blood 105, 40-48, doi:10.1182/blood-2004-04-1430 (2005).
    • (2005) Blood , vol.105 , pp. 40-48
    • Wysoczynski, M.1
  • 44
    • 33947393053 scopus 로고    scopus 로고
    • Regulation of CXCR4 signaling
    • Busillo, J. M. & Benovic, J. L. Regulation of CXCR4 signaling. Biochim Biophys Acta 1768, 952-963, doi:10.1016/j. bbamem.2006.11.002 (2007).
    • (2007) Biochim Biophys Acta , vol.1768 , pp. 952-963
    • Busillo, J.M.1    Benovic, J.L.2
  • 45
    • 84955468904 scopus 로고    scopus 로고
    • CXCR4 signaling is controlled by immobilization at the plasma membrane
    • Beletkaia, E. et al. CXCR4 signaling is controlled by immobilization at the plasma membrane. Biochim Biophys Acta 1863, 607-616, doi:10.1016/j.bbamcr.2015.12.020 (2016).
    • (2016) Biochim Biophys Acta , vol.1863 , pp. 607-616
    • Beletkaia, E.1
  • 46
    • 79951713324 scopus 로고    scopus 로고
    • Application of G protein-coupled receptor-heteromer identification technology to monitor beta-arrestin recruitment to G protein-coupled receptor heteromers
    • See, H. B., Seeber, R. M., Kocan, M., Eidne, K. A. & Pfleger, K. D. Application of G protein-coupled receptor-heteromer identification technology to monitor beta-arrestin recruitment to G protein-coupled receptor heteromers. Assay Drug Dev Technol 9, 21-30, doi:10.1089/adt.2010.0336 (2011).
    • (2011) Assay Drug Dev Technol , vol.9 , pp. 21-30
    • See, H.B.1    Seeber, R.M.2    Kocan, M.3    Eidne, K.A.4    Pfleger, K.D.5
  • 47
    • 78650172422 scopus 로고    scopus 로고
    • Beta2-Adrenergic receptor signaling in the cardiac myocyte is modulated by interactions with CXCR4
    • LaRocca, T. J. et al. beta2-Adrenergic receptor signaling in the cardiac myocyte is modulated by interactions with CXCR4. J Cardiovasc Pharmacol 56, 548-559, doi:10.1097/FJC.0b013e3181f713fe (2010).
    • (2010) J Cardiovasc Pharmacol , vol.56 , pp. 548-559
    • LaRocca, T.J.1
  • 48
    • 67650349084 scopus 로고    scopus 로고
    • CXCR7 heterodimerizes with CXCR4 and regulates CXCL12-mediated G protein signaling
    • Levoye, A., Balabanian, K., Baleux, F., Bachelerie, F. & Lagane, B. CXCR7 heterodimerizes with CXCR4 and regulates CXCL12-mediated G protein signaling. Blood 113, 6085-6093, doi:10.1182/blood-2008-12-196618 (2009).
    • (2009) Blood , vol.113 , pp. 6085-6093
    • Levoye, A.1    Balabanian, K.2    Baleux, F.3    Bachelerie, F.4    Lagane, B.5
  • 49
    • 80052768977 scopus 로고    scopus 로고
    • CXCR7/CXCR4 heterodimer constitutively recruits beta-arrestin to enhance cell migration
    • Decaillot, F. M. et al. CXCR7/CXCR4 heterodimer constitutively recruits beta-arrestin to enhance cell migration. J Biol Chem 286, 32188-32197, doi:10.1074/jbc.M111.277038 (2011).
    • (2011) J Biol Chem , vol.286 , pp. 32188-32197
    • Decaillot, F.M.1
  • 50
    • 84880586724 scopus 로고    scopus 로고
    • 3rd DNA repair mechanisms in dividing and non-dividing cells
    • Iyama, T. & Wilson, D. M. 3rd DNA repair mechanisms in dividing and non-dividing cells. DNA Repair (Amst) 12, 620-636, doi:10.1016/j.dnarep.2013.04.015 (2013).
    • (2013) DNA Repair (Amst) , vol.12 , pp. 620-636
    • Iyama, T.1    Wilson, D.M.2
  • 51
    • 80051741721 scopus 로고    scopus 로고
    • Efficient mutagenesis of the rhodopsin gene in rod photoreceptor neurons in mice
    • Chan, F., Hauswirth, W. W., Wensel, T. G. & Wilson, J. H. Efficient mutagenesis of the rhodopsin gene in rod photoreceptor neurons in mice. Nucleic Acids Res 39, 5955-5966, doi:10.1093/nar/gkr196 (2011).
    • (2011) Nucleic Acids Res , vol.39 , pp. 5955-5966
    • Chan, F.1    Hauswirth, W.W.2    Wensel, T.G.3    Wilson, J.H.4
  • 52
    • 84929276488 scopus 로고    scopus 로고
    • Efficient CRISPR-Cas9-mediated generation of knockin human pluripotent stem cells lacking undesired mutations at the targeted locus
    • Merkle, F. T. et al. Efficient CRISPR-Cas9-mediated generation of knockin human pluripotent stem cells lacking undesired mutations at the targeted locus. Cell Rep 11, 875-883, doi:10.1016/j.celrep.2015.04.007 (2015).
    • (2015) Cell Rep , vol.11 , pp. 875-883
    • Merkle, F.T.1
  • 53
    • 84975678715 scopus 로고    scopus 로고
    • Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system
    • Zetsche, B. et al. Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Cell 163, 759-771, doi:10.1016/j. cell.2015.09.038 (2015).
    • (2015) Cell , vol.163 , pp. 759-771
    • Zetsche, B.1
  • 54
    • 84955072791 scopus 로고    scopus 로고
    • Self-assembling nanoluc luciferase fragments as probes for protein aggregation in living cells
    • Zhao, J., Nelson, T. J., Vu, Q., Truong, T. & Stains, C. I. Self-Assembling NanoLuc Luciferase Fragments as Probes for Protein Aggregation in Living Cells. ACS Chem Biol 11, 132-138, doi:10.1021/acschembio.5b00758 (2016).
    • (2016) ACS Chem Biol , vol.11 , pp. 132-138
    • Zhao, J.1    Nelson, T.J.2    Vu, Q.3    Truong, T.4    Stains, C.I.5
  • 55
    • 84875604499 scopus 로고    scopus 로고
    • Conformational biosensors reveal GPCR signalling from endosomes
    • Irannejad, R. et al. Conformational biosensors reveal GPCR signalling from endosomes. Nature 495, 534-538, doi:10.1038/ nature12000 (2013).
    • (2013) Nature , vol.495 , pp. 534-538
    • Irannejad, R.1
  • 56
    • 84881464516 scopus 로고    scopus 로고
    • The bile acid receptor TGR5 does not interact with beta-arrestins or traffic to endosomes but transmits sustained signals from plasma membrane rafts
    • Jensen, D. D. et al. The bile acid receptor TGR5 does not interact with beta-arrestins or traffic to endosomes but transmits sustained signals from plasma membrane rafts. J Biol Chem 288, 22942-22960, doi:10.1074/jbc.M113.455774 (2013).
    • (2013) J Biol Chem , vol.288 , pp. 22942-22960
    • Jensen, D.D.1
  • 57
    • 84867234267 scopus 로고    scopus 로고
    • Sensitive and high resolution localization and tracking of membrane proteins in live cells with BRET
    • Lan, T. H., Liu, Q., Li, C., Wu, G. & Lambert, N. A. Sensitive and high resolution localization and tracking of membrane proteins in live cells with BRET. Traffic 13, 1450-1456, doi:10.1111/j.1600-0854.2012.01401.x (2012).
    • (2012) Traffic , vol.13 , pp. 1450-1456
    • Lan, T.H.1    Liu, Q.2    Li, C.3    Wu, G.4    Lambert, N.A.5
  • 58
    • 84978431584 scopus 로고    scopus 로고
    • Monitoring G protein-coupled receptor and [beta]-arrestin trafficking in live cells using enhanced bystander BRET
    • Namkung, Y. et al. Monitoring G protein-coupled receptor and [beta]-arrestin trafficking in live cells using enhanced bystander BRET. Nat Commun 7, doi:10.1038/ncomms12178 (2016).
    • (2016) Nat Commun , vol.7
    • Namkung, Y.1
  • 59
    • 78651295086 scopus 로고    scopus 로고
    • Rab GTPases bind at a common site within the angiotensin II type i receptor carboxylterminal tail: Evidence that Rab4 regulates receptor phosphorylation, desensitization, and resensitization
    • Esseltine, J. L., Dale, L. B. & Ferguson, S. S. Rab GTPases bind at a common site within the angiotensin II type I receptor carboxylterminal tail: evidence that Rab4 regulates receptor phosphorylation, desensitization, and resensitization. Mol Pharmacol 79, 175-184, doi:10.1124/mol.110.068379 (2011).
    • (2011) Mol Pharmacol , vol.79 , pp. 175-184
    • Esseltine, J.L.1    Dale, L.B.2    Ferguson, S.S.3
  • 60
    • 84876461130 scopus 로고    scopus 로고
    • Regulation of G protein-coupled receptor trafficking and signaling by Rab GTPases
    • Esseltine, J. L. & Ferguson, S. S. Regulation of G protein-coupled receptor trafficking and signaling by Rab GTPases. Small GTPases 4, 132-135, doi:10.4161/sgtp.24304 (2013).
    • (2013) Small GTPases , vol.4 , pp. 132-135
    • Esseltine, J.L.1    Ferguson, S.S.2
  • 61
    • 1842477154 scopus 로고    scopus 로고
    • Regulation of angiotensin II type 1A receptor intracellular retention, degradation, and recycling by Rab5, Rab7, and Rab11 GTPases
    • Dale, L. B., Seachrist, J. L., Babwah, A. V. & Ferguson, S. S. Regulation of angiotensin II type 1A receptor intracellular retention, degradation, and recycling by Rab5, Rab7, and Rab11 GTPases. J Biol Chem 279, 13110-13118, doi:10.1074/jbc.M313333200 (2004).
    • (2004) J Biol Chem , vol.279 , pp. 13110-13118
    • Dale, L.B.1    Seachrist, J.L.2    Babwah, A.V.3    Ferguson, S.S.4
  • 62
    • 0034282429 scopus 로고    scopus 로고
    • Beta 2-adrenergic receptor internalization, endosomal sorting, and plasma membrane recycling are regulated by rab GTPases
    • Seachrist, J. L., Anborgh, P. H. & Ferguson, S. S. beta 2-adrenergic receptor internalization, endosomal sorting, and plasma membrane recycling are regulated by rab GTPases. J Biol Chem 275, 27221-27228, doi:10.1074/jbc.M003657200 (2000).
    • (2000) J Biol Chem , vol.275 , pp. 27221-27228
    • Seachrist, J.L.1    Anborgh, P.H.2    Ferguson, S.S.3
  • 63
    • 78650216343 scopus 로고    scopus 로고
    • Multiplexing of multicolor bioluminescence resonance energy transfer
    • Breton, B. et al. Multiplexing of multicolor bioluminescence resonance energy transfer. Biophys J 99, 4037-4046, doi:10.1016/j. bpj.2010.10.025 (2010).
    • (2010) Biophys J , vol.99 , pp. 4037-4046
    • Breton, B.1
  • 64
    • 84884165315 scopus 로고    scopus 로고
    • DNA targeting specificity of RNA-guided Cas9 nucleases
    • Hsu, P. D. et al. DNA targeting specificity of RNA-guided Cas9 nucleases. Nat Biotechnol 31, 827-832, doi:10.1038/nbt.2647 (2013).
    • (2013) Nat Biotechnol , vol.31 , pp. 827-832
    • Hsu, P.D.1
  • 65
    • 0036079158 scopus 로고    scopus 로고
    • The human genome browser at UCSC
    • Kent, W. J. et al. The human genome browser at UCSC. Genome Res 12, 996-1006, doi:10.1101/gr.229102 (2002)
    • (2002) Genome Res , vol.12 , pp. 996-1006
    • Kent, W.J.1
  • 66
    • 0033003760 scopus 로고    scopus 로고
    • A simple statistical parameter for use in evaluation and validation of high throughput screening assays
    • Zhang, J. H., Chung, T. D. & Oldenburg, K. R. A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays. J Biomol Screen 4, 67-73 (1999).
    • (1999) J Biomol Screen , vol.4 , pp. 67-73
    • Zhang, J.H.1    Chung, T.D.2    Oldenburg, K.R.3
  • 67
    • 53649086041 scopus 로고    scopus 로고
    • Demonstration of improvements to the bioluminescence resonance energy transfer (BRET) technology for the monitoring of G protein-coupled receptors in live cells
    • Kocan, M., See, H. B., Seeber, R. M., Eidne, K. A. & Pfleger, K. D. Demonstration of improvements to the bioluminescence resonance energy transfer (BRET) technology for the monitoring of G protein-coupled receptors in live cells. J Biomol Screen 13, 888-898, doi:10.1177/1087057108324032 (2008).
    • (2008) J Biomol Screen , vol.13 , pp. 888-898
    • Kocan, M.1    See, H.B.2    Seeber, R.M.3    Eidne, K.A.4    Pfleger, K.D.5
  • 68
    • 80051471825 scopus 로고    scopus 로고
    • Heteromerization of angiotensin receptors changes trafficking and arrestin recruitment profiles
    • Porrello, E. R. et al. Heteromerization of angiotensin receptors changes trafficking and arrestin recruitment profiles. Cell Signal 23, 1767-1776, doi:10.1016/j.cellsig.2011.06.011 (2011).
    • (2011) Cell Signal , vol.23 , pp. 1767-1776
    • Porrello, E.R.1
  • 69
    • 64749087041 scopus 로고    scopus 로고
    • Agonist-independent interactions between beta-arrestins and mutant vasopressin type II receptors associated with nephrogenic syndrome of inappropriate antidiuresis
    • Kocan, M. et al. Agonist-independent interactions between beta-arrestins and mutant vasopressin type II receptors associated with nephrogenic syndrome of inappropriate antidiuresis. Mol Endocrinol 23, 559-571, doi:10.1210/me.2008-0321 (2009).
    • (2009) Mol Endocrinol , vol.23 , pp. 559-571
    • Kocan, M.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.