Sensitive and High Resolution Localization and Tracking of Membrane Proteins in Live Cells with BRET

Traffic

Volumn 13, Issue 11, 2012, Pages 1450-1456

  Lan, Tien Hung ^a   Liu, Qiuju ^a   Li, Chunman ^a   Wu, Guangyu ^a   Lambert, Nevin A ^a  

^a MEDICAL COLLEGE OF GEORGIA   (United States)

Author keywords

Adrenergic; Bioluminescence; Colocalization; GTPase; Topology; Trafficking

Indexed keywords

ADRENALIN; BETA 2 ADRENERGIC RECEPTOR; CHAPERONE; GUANOSINE TRIPHOSPHATASE; LIGAND; MEMBRANE PROTEIN; PLASMID DNA; RAPAMYCIN;

ARTICLE; BIOLUMINESCENCE; BIOLUMINESCENCE RESONANCE ENERGY TRANSFER; CELL MEMBRANE; CELL MEMBRANE TRANSPORT; CELL STRAIN HEK293; CONFOCAL MICROSCOPY; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; GOLGI COMPLEX; HUMAN; HUMAN CELL; MITOCHONDRIAL MEMBRANE; PRIORITY JOURNAL; PROTEIN LOCALIZATION;

BIOLUMINESCENCE RESONANCE ENERGY TRANSFER TECHNIQUES; HEK293 CELLS; HUMANS; MEMBRANE PROTEINS; PROTEIN TRANSPORT;

EID: 84867234267     PISSN: 13989219     EISSN: 16000854     Source Type: Journal    
DOI: 10.1111/j.1600-0854.2012.01401.x     Document Type: Article

References (26)

1. Rimon N, Schuldiner M. Getting the whole picture: combining throughput with content in microscopy. J Cell Sci 2011;124:3743-3751.
2. Lippincott-Schwartz J, Manley S. Putting super-resolution fluorescence microscopy to work. Nat Methods 2009;6:21-23.
3. Chiu VK, Bivona T, Hach A, Sajous JB, Silletti J, Wiener H, Johnson RL 2nd, Cox AD, Philips MR. Ras signalling on the endoplasmic reticulum and the Golgi. Nat Cell Biol 2002;4:343-350.
4. Violin JD, Ren XR, Lefkowitz RJ. G-protein-coupled receptor kinase specificity for beta-arrestin recruitment to the beta2-adrenergic receptor revealed by fluorescence resonance energy transfer. J Biol Chem 2006;281:20577-20588.
5. Rocks O, Peyker A, Kahms M, Verveer PJ, Koerner C, Lumbierres M, Kuhlmann J, Waldmann H, Wittinghofer A, Bastiaens PI. An acylation cycle regulates localization and activity of palmitoylated Ras isoforms. Science 2005;307:1746-1752.
6. Goodwin JS, Drake KR, Rogers C, Wright L, Lippincott-Schwartz J, Philips MR, Kenworthy AK. Depalmitoylated Ras traffics to and from the Golgi complex via a nonvesicular pathway. J Cell Biol 2005;170:261-272.
7. Rocks O, Gerauer M, Vartak N, Koch S, Huang ZP, Pechlivanis M, Kuhlmann J, Brunsveld L, Chandra A, Ellinger B, Waldmann H, Bastiaens PI. The palmitoylation machinery is a spatially organizing system for peripheral membrane proteins. Cell 2010;141:458-471.
8. Rocks O, Peyker A, Bastiaens PI. Spatio-temporal segregation of Ras signals: one ship, three anchors, many harbors. Curr Opin Cell Biol 2006;18:351-357.
9. Bergmann JE. Using temperature-sensitive mutants of VSV to study membrane protein biogenesis. Methods Cell Biol 1989;32:85-110.
10. Presley JF, Cole NB, Schroer TA, Hirschberg K, Zaal KJ, Lippincott-Schwartz J. ER-to-Golgi transport visualized in living cells. Nature 1997;389:81-85.
11. Komatsu T, Kukelyansky I, McCaffery JM, Ueno T, Varela LC, Inoue T. Organelle-specific, rapid induction of molecular activities and membrane tethering. Nat Methods 2010;7:206-208.
12. Leventis R, Silvius JR. Lipid-binding characteristics of the polybasic carboxy-terminal sequence of K-ras4B. Biochemistry 1998;37:7640-7648.
13. Yokoe H, Meyer T. Spatial dynamics of GFP-tagged proteins investigated by local fluorescence enhancement. Nat Biotechnol 1996;14:1252-1256.
14. Stauffer TP, Ahn S, Meyer T. Receptor-induced transient reduction in plasma membrane PtdIns(4, 5)P2 concentration monitored in living cells. Curr Biol 1998;8:343-346.
15. Fung BK, Stryer L. Surface density determination in membranes by fluorescence energy transfer. Biochemistry 1978;17:5241-5248.
16. Wolber PK, Hudson BS. An analytic solution to the Forster energy transfer problem in two dimensions. Biophys J 1979;28:197-210.
17. Shirihai OS, Gregory T, Yu C, Orkin SH, Weiss MJ. ABC-me: a novel mitochondrial transporter induced by GATA-1 during erythroid differentiation. EMBO J 2000;19:2492-2502.
18. Lorenz H, Hailey DW, Lippincott-Schwartz J. Fluorescence protease protection of GFP chimeras to reveal protein topology and subcellular localization. Nat Methods 2006;3:205-210.
19. Hanson MA, Cherezov V, Griffith MT, Roth CB, Jaakola VP, Chien EY, Velasquez J, Kuhn P, Stevens RC. A specific cholesterol binding site is established by the 2.8 A structure of the human beta2-adrenergic receptor. Structure 2008;16:897-905.
20. Kobayashi H, Ogawa K, Yao R, Lichtarge O, Bouvier M. Functional rescue of beta-adrenoceptor dimerization and trafficking by pharmacological chaperones. Traffic 2009;10:1019-1033.
21. Cheng T, Zhao Y, Li X, Lin F, Xu Y, Zhang X, Li Y, Wang R, Lai L. Computation of octanol-water partition coefficients by guiding an additive model with knowledge. J Chem Inf Model 2007;47:2140-2148.
22. Teruel MN, Meyer T. Translocation and reversible localization of signaling proteins: a dynamic future for signal transduction. Cell 2000;103:181-184.
23. Engelman DM. Membranes are more mosaic than fluid. Nature 2005;438:578-580.
24. Qin K, Dong C, Wu G, Lambert NA. Inactive-state preassembly of G(q)-coupled receptors and G(q) heterotrimers. Nat Chem Biol 2011;7:740-747.
25. Dragulescu-Andrasi A, Chan CT, De A, Massoud TF, Gambhir SS. Bioluminescence resonance energy transfer (BRET) imaging of protein-protein interactions within deep tissues of living subjects. Proc Natl Acad Sci USA 2011;108:12060-12065.
26. Breton B, Sauvageau E, Zhou J, Bonin H, Le Gouill C, Bouvier M. Multiplexing of multicolor bioluminescence resonance energy transfer. Biophys J 2010;99:4037-4046.