NanoBRET-A Novel BRET Platform for the Analysis of Protein-Protein Interactions

ACS Chemical Biology

Volumn 10, Issue 8, 2015, Pages 1797-1804

  Machleidt, Thomas ^a   Woodroofe, Carolyn C ^b   Schwinn, Marie K ^a   Méndez, Jacqui ^a   Robers, Matthew B ^a   Zimmerman, Kris ^a   Otto, Paul ^a   Daniels, Danette L ^a   Kirkland, Thomas A ^b   Wood, Keith V ^a  

^a PROMEGA CORPORATION   (United States)

^b Promega Biosciences Incorporated ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE; RENILLA LUCIFERIN 2 MONOOXYGENASE;

CHEMISTRY; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENETICS; HEK293 CELL LINE; HELA CELL LINE; HUMAN; METABOLISM; PROCEDURES; PROTEIN ANALYSIS; PROTEIN ENGINEERING; PROTEIN PROTEIN INTERACTION;

FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; HEK293 CELLS; HELA CELLS; HUMANS; LUCIFERASES, RENILLA; PROTEIN ENGINEERING; PROTEIN INTERACTION MAPPING; PROTEIN INTERACTION MAPS;

EID: 84939802598     PISSN: 15548929     EISSN: 15548937     Source Type: Journal    
DOI: 10.1021/acschembio.5b00143     Document Type: Article

References (25)

1. Ciruela, F. (2008) Fluorescence-based methods in the study of protein-protein interactions in living cells Curr. Opin. Biotechnol. 19, 338-343
2. Angers, S., Salahpour, A., Joly, E., Hilairet, S., Chelsky, D., Dennis, M., and Bouvier, M. (2000) Detection of beta 2-adrenergic receptor dimerization in living cells using bioluminescence resonance energy transfer (BRET) Proc. Natl. Acad. Sci. U.S.A. 97, 3684-3689
3. Xu, Y., Piston, D. W., and Johnson, C. H. (1999) A bioluminescence resonance energy transfer (BRET) system: application to interacting circadian clock proteins Proc. Natl. Acad. Sci. U.S.A. 96, 151-156
4. Wampler, J. E., Hori, K., Lee, J. W., and Cormier, M. J. (1971) Structured bioluminescence. Two emitters during both the in vitro and the in vivo bioluminescence of the sea pansy, Renilla Biochemistry 10, 2903-2909
5. Pfleger, K. D. and Eidne, K. A. (2006) Illuminating insights into protein-protein interactions using bioluminescence resonance energy transfer (BRET) Nat. Methods 3, 165-174
6. Bertrand, L., Parent, S., Caron, M., Legault, M., Joly, E., Angers, S., Bouvier, M., Brown, M., Houle, B., and Menard, L. (2002) The BRET2/arrestin assay in stable recombinant cells: a platform to screen for compounds that interact with G protein-coupled receptors (GPCRS) J. Recept. Signal Transduction Res. 22, 533-541
7. Hamdan, F. F., Audet, M., Garneau, P., Pelletier, J., and Bouvier, M. (2005) High-throughput screening of G protein-coupled receptor antagonists using a bioluminescence resonance energy transfer 1-based beta-arrestin2 recruitment assay J. Biomol. Screening 10, 463-475
8. Loening, A. M., Fenn, T. D., Wu, A. M., and Gambhir, S. S. (2006) Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and light output Protein Eng., Des. Sel. 19, 391-400
9. Kocan, M., See, H. B., Seeber, R. M., Eidne, K. A., and Pfleger, K. D. (2008) Demonstration of improvements to the bioluminescence resonance energy transfer (BRET) technology for the monitoring of G protein-coupled receptors in live cells J. Biomol. Screening 13, 888-898
10. Hall, M. P., Unch, J., Binkowski, B. F., Valley, M. P., Butler, B. L., Wood, M. G., Otto, P., Zimmerman, K., Vidugiris, G., Machleidt, T., Robers, M. B., Benink, H. A., Eggers, C. T., Slater, M. R., Meisenheimer, P. L., Klaubert, D. H., Fan, F., Encell, L. P., and Wood, K. V. (2012) Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate ACS Chem. Biol. 7, 1848-1857
11. Los, G. V., Encell, L. P., McDougall, M. G., Hartzell, D. D., Karassina, N., Zimprich, C., Wood, M. G., Learish, R., Ohana, R. F., Urh, M., Simpson, D., Mendez, J., Zimmerman, K., Otto, P., Vidugiris, G., Zhu, J., Darzins, A., Klaubert, D. H., Bulleit, R. F., and Wood, K. V. (2008) HaloTag: a novel protein labeling technology for cell imaging and protein analysis ACS Chem. Biol. 3, 373-382
12. Shagin, D. A., Barsova, E. V., Yanushevich, Y. G., Fradkov, A. F., Lukyanov, K. A., Labas, Y. A., Semenova, T. N., Ugalde, J. A., Meyers, A., Nunez, J. M., Widder, E. A., Lukyanov, S. A., and Matz, M. V. (2004) GFP-like proteins as ubiquitous metazoan superfamily: evolution of functional features and structural complexity Mol. Biol. Evol. 21, 841-850
13. Merzlyak, E. M., Goedhart, J., Shcherbo, D., Bulina, M. E., Shcheglov, A. S., Fradkov, A. F., Gaintzeva, A., Lukyanov, K. A., Lukyanov, S., Gadella, T. W., and Chudakov, D. M. (2007) Bright monomeric red fluorescent protein with an extended fluorescence lifetime Nat. Methods 4, 555-557
14. Nagai, T., Ibata, K., Park, E. S., Kubota, M., Mikoshiba, K., and Miyawaki, A. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications Nat. Biotechnol. 20, 87-90
15. Shaner, N. C., Steinbach, P. A., and Tsien, R. Y. (2005) A guide to choosing fluorescent proteins Nat. Methods 2, 905-909
16. Galarneau, A., Primeau, M., Trudeau, L. E., and Michnick, S. W. (2002) Beta-lactamase protein fragment complementation assays as in vivo and in vitro sensors of protein protein interactions Nat. Biotechnol. 20, 619-622
17. Zhang, J. H., Chung, T. D., and Oldenburg, K. R. (1999) A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays J. Biomol. Screening 4, 67-73
18. Wells, J. A. and McClendon, C. L. (2007) Reaching for high-hanging fruit in drug discovery at protein-protein interfaces Nature 450, 1001-1009
19. Sanchez, R., Meslamani, J., and Zhou, M. M. (2014) The bromodomain: from epigenome reader to druggable target Biochim. Biophys. Acta 1839, 676-685
20. Dawson, M. A., Kouzarides, T., and Huntly, B. J. (2012) Targeting epigenetic readers in cancer N. Engl. J. Med. 367, 647-657
21. Filippakopoulos, P., Qi, J., Picaud, S., Shen, Y., Smith, W. B., Fedorov, O., Morse, E. M., Keates, T., Hickman, T. T., Felletar, I., Philpott, M., Munro, S., McKeown, M. R., Wang, Y., Christie, A. L., West, N., Cameron, M. J., Schwartz, B., Heightman, T. D., La Thangue, N., French, C. A., Wiest, O., Kung, A. L., Knapp, S., and Bradner, J. E. (2010) Selective inhibition of BET bromodomains Nature 468, 1067-1073
22. Dawson, M. A., Prinjha, R. K., Dittmann, A., Giotopoulos, G., Bantscheff, M., Chan, W. I., Robson, S. C., Chung, C. W., Hopf, C., Savitski, M. M., Huthmacher, C., Gudgin, E., Lugo, D., Beinke, S., Chapman, T. D., Roberts, E. J., Soden, P. E., Auger, K. R., Mirguet, O., Doehner, K., Delwel, R., Burnett, A. K., Jeffrey, P., Drewes, G., Lee, K., Huntly, B. J., and Kouzarides, T. (2011) Inhibition of BET recruitment to chromatin as an effective treatment for MLL-fusion leukaemia Nature 478, 529-533
23. Oakley, R. H., Laporte, S. A., Holt, J. A., Barak, L. S., and Caron, M. G. (1999) Association of beta-arrestin with G protein-coupled receptors during clathrin-mediated endocytosis dictates the profile of receptor resensitization J. Biol. Chem. 274, 32248-32257
24. Laporte, S. A., Oakley, R. H., Zhang, J., Holt, J. A., Ferguson, S. S., Caron, M. G., and Barak, L. S. (1999) The beta2-adrenergic receptor/betaarrestin complex recruits the clathrin adaptor AP-2 during endocytosis Proc. Natl. Acad. Sci. U.S.A. 96, 3712-3717
25. Mahen, R., Koch, B., Wachsmuth, M., Politi, A. Z., Perez-Gonzalez, A., Mergenthaler, J., Cai, Y., and Ellenberg, J. (2014) Comparative assessment of fluorescent transgene methods for quantitative imaging in human cells Mol. Biol. Cell 25, 3610