Application of BRET to monitor ligand binding to GPCRs

Nature Methods

Volumn 12, Issue 7, 2015, Pages 661-663

  Stoddart, Leigh A ^a   Johnstone, Elizabeth K M ^b,c   Wheal, Amanda J ^a   Goulding, Joëlle ^a   Robers, Matthew B ^d   MacHleidt, Thomas ^d   Wood, Keith V ^d   Hill, Stephen J ^a,b,c   Pfleger, Kevin D G ^b,c  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^b UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

^c UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

^d PROMEGA CORPORATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

3 ISOPROPYLAMINO 1 (7 METHYL 4 INDANYLOXY) 2 BUTANOL; 4 (3 TERT BUTYLAMINO 2 HYDROXYPROPOXY) 2 BENZIMIDAZOLONE; FLUORESCENT DYE; G PROTEIN COUPLED RECEPTOR; ISOPRENALINE; LIGAND; LUCIFERASE; NANOLUC; PROPRANOLOL; UNCLASSIFIED DRUG; BETA 2 ADRENERGIC RECEPTOR;

AMINO TERMINAL SEQUENCE; ARTICLE; BIOLUMINESCENCE RESONANCE ENERGY TRANSFER; CELL GROWTH; CELL SURFACE; CONTROLLED STUDY; DISSOCIATION CONSTANT; HEK293 CELL LINE; HUMAN; HUMAN CELL; LIGAND BINDING; LUMINESCENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; FLUORESCENCE; METABOLISM; PROCEDURES;

BIOLUMINESCENCE RESONANCE ENERGY TRANSFER TECHNIQUES; FLUORESCENCE; HEK293 CELLS; HUMANS; LIGANDS; RECEPTORS, ADRENERGIC, BETA-2; RECEPTORS, G-PROTEIN-COUPLED;

EID: 84934434629     PISSN: 15487091     EISSN: 15487105     Source Type: Journal    
DOI: 10.1038/nmeth.3398     Document Type: Article

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