Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens

Cell

Volumn 167, Issue 7, 2016, Pages 1853-1866.e17

  Dixit, Atray ^a,b   Parnas, Oren ^a   Li, Biyu ^a   Chen, Jenny ^a,b   Fulco, Charles P ^a,d   Jerby Arnon, Livnat ^a   Marjanovic, Nemanja D ^a,c   Dionne, Danielle ^a   Burks, Tyler ^a   Raychowdhury, Raktima ^a   Adamson, Britt ^e   Norman, Thomas M ^e   Lander, Eric S ^a,d,f   Weissman, Jonathan S ^e,g   Friedman, Nir ^a,h   Regev, Aviv ^a,f,g  

^a BROAD INSTITUTE OF MIT AND HARVARD   (United States)

^b HARVARD MIT DIVISION OF HEALTH SCIENCES AND TECHNOLOGY   (United States)

^c Massachusetts Institute of Technology Cambridge   (United States)

^d HARVARD MEDICAL SCHOOL   (United States)

^e UNIVERSITY OF CALIFORNIA   (United States)

^f MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^g HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^h HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

CRISPR; epistasis; genetic interactions; pooled screen; single cell RNA seq

Indexed keywords

ACTIVATING TRANSCRIPTION FACTOR 3; CCAAT ENHANCER BINDING PROTEIN BETA; CRISPR ASSOCIATED PROTEIN; CRISPR ASSOCIATED PROTEIN 9; CYCLIC AMP RESPONSIVE ELEMENT BINDING PROTEIN; EARLY GROWTH RESPONSE FACTOR 1; GAMMA INTERFERON; GUIDE RNA; HYPOXIA INDUCIBLE FACTOR 1; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INTERFERON REGULATORY FACTOR 1; INTERFERON REGULATORY FACTOR 2; INTERFERON REGULATORY FACTOR 4; LIPOPOLYSACCHARIDE; RIBOSOME PROTEIN; STAT1 PROTEIN; STAT2 PROTEIN; STAT3 PROTEIN; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR E2F4; TRANSCRIPTION FACTOR ELF 1; TRANSCRIPTION FACTOR ELK 1; TRANSCRIPTION FACTOR GABPA; TRANSCRIPTION FACTOR JUNB; TRANSCRIPTION FACTOR PU 1; TRANSCRIPTION FACTOR REL; TRANSCRIPTION FACTOR RELA; TRANSCRIPTION FACTOR RUNX1; TRANSCRIPTION FACTOR YY1; TUMOR NECROSIS FACTOR; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIGEN PRESENTATION; ARTICLE; BONE MARROW DERIVED DENDRITIC CELL; CELL CYCLE REGULATION; CELL DIFFERENTIATION; CONTROLLED STUDY; CRISPR CAS SYSTEM; CYTOSKELETON; DNA BARCODING; FEMALE; GENE EXPRESSION PROFILING; GENE FUNCTION; GENE INTERACTION; GENE LOCUS; GENE TARGETING; GENETIC ASSOCIATION; GENETIC MANIPULATION; GENETIC SCREENING; GENOME ANALYSIS; HELA CELL LINE; HUMAN; HUMAN CELL; IMMUNE ACTIVATION; IMMUNITY; INNATE IMMUNITY; K-562 CELL LINE; MITOCHONDRIAL BIOGENESIS; MITOCHONDRION; MITOSIS INHIBITION; MOUSE; NEGATIVE FEEDBACK; NONHUMAN; POSITIVE FEEDBACK; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; REPRODUCTIVE FITNESS; SCALE OF MASSIVELY PARALLEL SINGLE CELL RNA SEQUENCING; SEQUENCE ANALYSIS; TRANSCRIPTION REGULATION; VIRUS IMMUNITY; ANIMAL; CELL CYCLE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; FEEDBACK SYSTEM; GENE SILENCING; METABOLISM; PROCEDURES; SINGLE CELL ANALYSIS; TRANSGENIC MOUSE;

ANIMALS; CELL CYCLE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; FEEDBACK; GENE EXPRESSION PROFILING; GENE KNOCKDOWN TECHNIQUES; HUMANS; K562 CELLS; MICE; MICE, TRANSGENIC; SEQUENCE ANALYSIS, RNA; SINGLE-CELL ANALYSIS; TRANSCRIPTION FACTORS;

EID: 85006488344     PISSN: 00928674     EISSN: 10974172     Source Type: Journal    
DOI: 10.1016/j.cell.2016.11.038     Document Type: Article

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