A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response

Cell

Volumn 167, Issue 7, 2016, Pages 1867-1882.e21

  Adamson, Britt ^a,b   Norman, Thomas M ^a,b   Jost, Marco ^a,b,c   Cho, Min Y ^a,b   Nuñez, James K ^a,b   Chen, Yuwen ^a,b   Villalta, Jacqueline E ^a,b   Gilbert, Luke A ^a,b   Horlbeck, Max A ^a,b   Hein, Marco Y ^a,b   Pak, Ryan A ^a,d   Gray, Andrew N ^c   Gross, Carol A ^a,c   Dixit, Atray ^e,f   Parnas, Oren ^f   Regev, Aviv ^f,g   Weissman, Jonathan S ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

^e HARVARD MIT DIVISION OF HEALTH SCIENCES AND TECHNOLOGY   (United States)

^f BROAD INSTITUTE OF MIT AND HARVARD   (United States)

^g MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

cell to cell heterogeneity; CRIPSRi; CRISPR; genome scale screening; single cell genomics; Single cell RNA seq; unfolded protein response

Indexed keywords

ACTIVATING TRANSCRIPTION FACTOR 6; AMINO ACID TRANSFER RNA LIGASE; DNA DIRECTED RNA POLYMERASE III; GROWTH ARREST AND DNA DAMAGE INDUCIBLE PROTEIN 153; GUIDE RNA; PROTEIN; PROTEIN IRE1; PROTEIN IRE1 ALPHA; SEC61A1 PROTEIN; SEC61B PROTEIN; SEL1L PROTEIN; SRP68 PROTEIN; SRP72 PROTEIN; SRPR PROTEIN; SRPRB PROTEIN; SYVN1 PROTEIN; THAPSIGARGIN; TRANSCRIPTOME; TRANSLOCON; TUNICAMYCIN; UNCLASSIFIED DRUG; ERN1 PROTEIN, HUMAN; PROTEIN SERINE THREONINE KINASE; RIBONUCLEASE;

ANIMAL CELL; ARTICLE; CELL CYCLE; CELL FUNCTION; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; COMPARATIVE STUDY; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; ENDOPLASMIC RETICULUM STRESS; FEEDBACK SYSTEM; GENE INTERACTION; GENE REPRESSION; GLYCOSYLATION; HOMEOSTASIS; NOISE; NONHUMAN; PHENOTYPE; POLYADENYLATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN FOLDING; PROTEIN TARGETING; RANDOM FOREST; REGULON; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SEQUENCE; SEQUENCE HOMOLOGY; SINGLE CELL ANALYSIS; UBIQUITINATION; UNFOLDED PROTEIN RESPONSE; UPREGULATION; ANIMAL; GENETIC TRANSCRIPTION; HUMAN; METABOLISM; MOLECULAR MODEL; PROCEDURES; SEQUENCE ANALYSIS;

ANIMALS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; ENDORIBONUCLEASES; FEEDBACK; HUMANS; MODELS, MOLECULAR; PROTEIN-SERINE-THREONINE KINASES; RNA, GUIDE; SEQUENCE ANALYSIS, RNA; SINGLE-CELL ANALYSIS; TRANSCRIPTION, GENETIC; UNFOLDED PROTEIN RESPONSE;

EID: 85006345820     PISSN: 00928674     EISSN: 10974172     Source Type: Journal    
DOI: 10.1016/j.cell.2016.11.048     Document Type: Article

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