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Volumn 347, Issue 6222, 2015, Pages

Combinatorial labeling of single cells for gene expression cytometry

Author keywords

[No Author keywords available]

Indexed keywords

CELL PROTEIN; CYTOKINE; MEMBRANE PROTEIN; TRANSCRIPTION FACTOR; COMPLEMENTARY DNA; MESSENGER RNA; MICROSPHERE;

EID: 84922311090     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1258367     Document Type: Article
Times cited : (333)

References (46)
  • 1
    • 79959403670 scopus 로고    scopus 로고
    • Characterization of the single-cell transcriptional landscape by highly multiplex RNA-seq
    • pmid: 21543516
    • S. Islam et al., Characterization of the single-cell transcriptional landscape by highly multiplex RNA-seq. Genome Res. 21, 1160-1167 (2011). doi: 10.1101/gr.110882.110; pmid: 21543516
    • (2011) Genome Res. , vol.21 , pp. 1160-1167
    • Islam, S.1
  • 2
    • 84878997106 scopus 로고    scopus 로고
    • Single-cell transcriptomics reveals bimodality in expression and splicing in immune cells
    • pmid: 23685454
    • A. K. Shalek et al., Single-cell transcriptomics reveals bimodality in expression and splicing in immune cells. Nature 498, 236-240 (2013). doi: 10.1038/nature12172; pmid: 23685454
    • (2013) Nature , vol.498 , pp. 236-240
    • Shalek, A.K.1
  • 3
    • 84893910301 scopus 로고    scopus 로고
    • Quantitative assessment of single-cell RNAsequencing methods
    • pmid: 24141493
    • A. R. Wu et al., Quantitative assessment of single-cell RNAsequencing methods. Nat. Methods 11, 41-46 (2014). doi: 10.1038/nmeth.2694; pmid: 24141493
    • (2014) Nat. Methods , vol.11 , pp. 41-46
    • Wu, A.R.1
  • 4
    • 84900529199 scopus 로고    scopus 로고
    • Reconstructing lineage hierarchies of the distal lung epithelium using single-cell RNA-seq
    • pmid: 24739965
    • B. Treutlein et al., Reconstructing lineage hierarchies of the distal lung epithelium using single-cell RNA-seq. Nature 509, 371-375 (2014). doi: 10.1038/nature13173; pmid: 24739965
    • (2014) Nature , vol.509 , pp. 371-375
    • Treutlein, B.1
  • 5
    • 84893905629 scopus 로고    scopus 로고
    • Massively parallel single-cell RNA-seq for marker-free decomposition of tissues into cell types
    • pmid: 24531970
    • D. A. Jaitin et al., Massively parallel single-cell RNA-seq for marker-free decomposition of tissues into cell types. Science 343, 776-779 (2014). doi: 10.1126/science.1247651; pmid: 24531970
    • (2014) Science , vol.343 , pp. 776-779
    • Jaitin, D.A.1
  • 6
    • 84859602728 scopus 로고    scopus 로고
    • Microfluidic single-cell real-time PCR for comparative analysis of gene expression patterns
    • pmid: 22481529
    • V. Sanchez-Freire, A. D. Ebert, T. Kalisky, S. R. Quake, J. C. Wu, Microfluidic single-cell real-time PCR for comparative analysis of gene expression patterns. Nat. Protoc. 7, 829-838 (2012). doi: 10.1038/nprot.2012.021; pmid: 22481529
    • (2012) Nat. Protoc. , vol.7 , pp. 829-838
    • Sanchez-Freire, V.1    Ebert, A.D.2    Kalisky, T.3    Quake, S.R.4    Wu, J.C.5
  • 7
    • 84895069488 scopus 로고    scopus 로고
    • Quantitative single-cell RNA-seq with unique molecular identifiers
    • pmid: 24363023
    • S. Islam et al., Quantitative single-cell RNA-seq with unique molecular identifiers. Nat. Methods 11, 163-166 (2014). doi: 10.1038/nmeth.2772; pmid: 24363023
    • (2014) Nat. Methods , vol.11 , pp. 163-166
    • Islam, S.1
  • 8
    • 84903185013 scopus 로고    scopus 로고
    • Single-cell RNA-seq reveals dynamic paracrine control of cellular variation
    • pmid: 24919153
    • A. K. Shalek et al., Single-cell RNA-seq reveals dynamic paracrine control of cellular variation. Nature 510, 363-369 (2014). pmid: 24919153
    • (2014) Nature , vol.510 , pp. 363-369
    • Shalek, A.K.1
  • 9
    • 84902668801 scopus 로고    scopus 로고
    • Single-cell RNA-seq highlights intratumoral heterogeneity in primary glioblastoma
    • pmid: 24925914
    • A. P. Patel et al., Single-cell RNA-seq highlights intratumoral heterogeneity in primary glioblastoma. Science 344, 1396-1401 (2014). doi: 10.1126/science.1254257; pmid: 24925914
    • (2014) Science , vol.344 , pp. 1396-1401
    • Patel, A.P.1
  • 10
    • 84882585786 scopus 로고    scopus 로고
    • Ultrahigh-throughput Mammalian single-cell reverse-transcriptase polymerase chain reaction in microfluidic drops
    • pmid: 23885761
    • D. J. Eastburn, A. Sciambi, A. R. Abate, Ultrahigh-throughput Mammalian single-cell reverse-transcriptase polymerase chain reaction in microfluidic drops. Anal. Chem. 85, 8016- 8021 (2013). doi: 10.1021/ac402057q; pmid: 23885761
    • (2013) Anal. Chem. , vol.85 , pp. 8016-8021
    • Eastburn, D.J.1    Sciambi, A.2    Abate, A.R.3
  • 11
    • 84897077889 scopus 로고    scopus 로고
    • Discriminating cellular heterogeneity using microwell-based RNA cytometry
    • pmid: 24667995
    • I. K. Dimov et al., Discriminating cellular heterogeneity using microwell-based RNA cytometry. Nat. Commun. 5, 3451 (2014). doi: 10.1038/ncomms4451; pmid: 24667995
    • (2014) Nat. Commun. , vol.5 , pp. 3451
    • Dimov, I.K.1
  • 12
    • 84859893070 scopus 로고    scopus 로고
    • Massively parallel single-molecule and single-cell emulsion reverse transcription polymerase chain reaction using agarose droplet microfluidics
    • pmid: 22455457
    • H. Zhang, G. Jenkins, Y. Zou, Z. Zhu, C. J. Yang, Massively parallel single-molecule and single-cell emulsion reverse transcription polymerase chain reaction using agarose droplet microfluidics. Anal. Chem. 84, 3599-3606 (2012). doi: 10.1021/ac2033084; pmid: 22455457
    • (2012) Anal. Chem. , vol.84 , pp. 3599-3606
    • Zhang, H.1    Jenkins, G.2    Zou, Y.3    Zhu, Z.4    Yang, C.J.5
  • 13
    • 79959327151 scopus 로고    scopus 로고
    • Counting individual DNA molecules by the stochastic attachment of diverse labels
    • pmid: 21562209
    • G. K. Fu, J. Hu, P. H. Wang, S. P. Fodor, Counting individual DNA molecules by the stochastic attachment of diverse labels. Proc. Natl. Acad. Sci. U.S.A. 108, 9026-9031 (2011). doi: 10.1073/pnas.1017621108; pmid: 21562209
    • (2011) Proc. Natl. Acad. Sci. U.S.A. , vol.108 , pp. 9026-9031
    • Fu, G.K.1    Hu, J.2    Wang, P.H.3    Fodor, S.P.4
  • 14
    • 84856484968 scopus 로고    scopus 로고
    • Counting absolute numbers of molecules using unique molecular identifiers
    • pmid: 22101854
    • T. Kivioja et al., Counting absolute numbers of molecules using unique molecular identifiers. Nat. Methods 9, 72-74 (2011). doi: 10.1038/nmeth.1778; pmid: 22101854
    • (2011) Nat. Methods , vol.9 , pp. 72-74
    • Kivioja, T.1
  • 15
    • 84896525250 scopus 로고    scopus 로고
    • Digital encoding of cellular mRNAs enabling precise and absolute gene expression measurement by single-molecule counting
    • pmid: 24579851
    • G. K. Fu, J. Wilhelmy, D. Stern, H. C. Fan, S. P. Fodor, Digital encoding of cellular mRNAs enabling precise and absolute gene expression measurement by single-molecule counting. Anal. Chem. 86, 2867-2870 (2014). doi: 10.1021/ac500459p; pmid: 24579851
    • (2014) Anal. Chem. , vol.86 , pp. 2867-2870
    • Fu, G.K.1    Wilhelmy, J.2    Stern, D.3    Fan, H.C.4    Fodor, S.P.5
  • 16
    • 84893473154 scopus 로고    scopus 로고
    • Molecular indexing enables quantitative targeted RNA sequencing and reveals poor efficiencies in standard library preparations
    • pmid: 24449890
    • G. K. Fu et al ., Molecular indexing enables quantitative targeted RNA sequencing and reveals poor efficiencies in standard library preparations. Proc. Natl. Acad. Sci. U.S.A. 111, 1891-1896 (2014). doi: 10.1073/pnas.1323732111; pmid: 24449890
    • (2014) Proc. Natl. Acad. Sci. U.S.A. , vol.111 , pp. 1891-1896
    • Fu, G.K.1
  • 17
    • 79959353421 scopus 로고    scopus 로고
    • Detection and quantification of rare mutations with massively parallel sequencing
    • pmid: 21586637
    • I. Kinde, J. Wu, N. Papadopoulos, K. W. Kinzler, B. Vogelstein, Detection and quantification of rare mutations with massively parallel sequencing. Proc. Natl. Acad. Sci. U.S.A. 108, 9530-9535 (2011). doi: 10.1073/pnas.1105422108; pmid: 21586637
    • (2011) Proc. Natl. Acad. Sci. U.S.A. , vol.108 , pp. 9530-9535
    • Kinde, I.1    Wu, J.2    Papadopoulos, N.3    Kinzler, K.W.4    Vogelstein, B.5
  • 18
    • 0034671678 scopus 로고    scopus 로고
    • Quantitative analysis of globin gene induction in single human erythroleukemic cells
    • pmid: 11121491
    • R. D. Smith, J. D. Malley, A. N. Schechter, Quantitative analysis of globin gene induction in single human erythroleukemic cells. Nucleic Acids Res. 28, 4998-5004 (2000). doi: 10.1093/nar/28.24.4998; pmid: 11121491
    • (2000) Nucleic Acids Res. , vol.28 , pp. 4998-5004
    • Smith, R.D.1    Malley, J.D.2    Schechter, A.N.3
  • 19
    • 84874209670 scopus 로고    scopus 로고
    • Advances in human B cell phenotypic profiling
    • pmid: 23087687
    • D. A. Kaminski, C.Wei, Y. Qian, A. F. Rosenberg, I. Sanz, Advances in human B cell phenotypic profiling. Front. Immunol. 3, 302 (2012). doi: 10.3389/fimmu.2012.00302; pmid: 23087687
    • (2012) Front. Immunol. , vol.3 , pp. 302
    • Kaminski, D.A.1    Wei, C.2    Qian, Y.3    Rosenberg, A.F.4    Sanz, I.5
  • 20
    • 26444531971 scopus 로고    scopus 로고
    • Distinct gene expression profiles in different B-cell compartments in human peripheral lymphoid organs
    • pmid: 15369600
    • Y. Shen et al., Distinct gene expression profiles in different B-cell compartments in human peripheral lymphoid organs. BMC Immunol. 5, 20 (2004). doi: 10.1186/1471-2172-5-20; pmid: 15369600
    • (2004) BMC Immunol. , vol.5 , pp. 20
    • Shen, Y.1
  • 21
    • 84879511309 scopus 로고    scopus 로고
    • Correlation of gene expression and genome mutation in single B-cells
    • pmid: 23840752
    • J. A. Weinstein, X. Zeng, Y. H. Chien, S. R. Quake, Correlation of gene expression and genome mutation in single B-cells. PLOS ONE 8, e67624 (2013). doi: 10.1371/journal.pone.0067624; pmid: 23840752
    • (2013) PLOS ONE , vol.8 , pp. e67624
    • Weinstein, J.A.1    Zeng, X.2    Chien, Y.H.3    Quake, S.R.4
  • 23
    • 47649124124 scopus 로고    scopus 로고
    • A global view of gene activity and alternative splicing by deep sequencing of the human transcriptome
    • pmid: 18599741
    • M. Sultan et al., A global view of gene activity and alternative splicing by deep sequencing of the human transcriptome. Science 321, 956-960 (2008). doi: 10.1126/science.1160342; pmid: 18599741
    • (2008) Science , vol.321 , pp. 956-960
    • Sultan, M.1
  • 24
    • 29144486808 scopus 로고    scopus 로고
    • Identification of T cell-restricted genes, and signatures for different T cell responses, using a comprehensive collection of microarray datasets
    • pmid: 16339519
    • T. Chtanova et al., Identification of T cell-restricted genes, and signatures for different T cell responses, using a comprehensive collection of microarray datasets. J. Immunol. 175, 7837-7847 (2005). doi: 10.4049/jimmunol.175.12.7837; pmid: 16339519
    • (2005) J. Immunol. , vol.175 , pp. 7837-7847
    • Chtanova, T.1
  • 25
    • 2942755979 scopus 로고    scopus 로고
    • T follicular helper cells express a distinctive transcriptional profile, reflecting their role as non-Th1/Th2 effector cells that provide help for B cells
    • pmid: 15210760
    • T. Chtanova et al., T follicular helper cells express a distinctive transcriptional profile, reflecting their role as non-Th1/Th2 effector cells that provide help for B cells. J. Immunol. 173, 68-78 (2004). doi: 10.4049/jimmunol.173.1.68; pmid: 15210760
    • (2004) J. Immunol. , vol.173 , pp. 68-78
    • Chtanova, T.1
  • 26
    • 78049414864 scopus 로고    scopus 로고
    • Molecular profiling of cytomegalovirusinduced human CD8+ T cell differentiation
    • pmid: 20921622
    • K. M. Hertoghs et al., Molecular profiling of cytomegalovirusinduced human CD8+ T cell differentiation. J. Clin. Invest. 120, 4077-4090 (2010). doi: 10.1172/JCI42758; pmid: 20921622
    • (2010) J. Clin. Invest. , vol.120 , pp. 4077-4090
    • Hertoghs, K.M.1
  • 27
    • 66449133523 scopus 로고    scopus 로고
    • Frequency and phenotype of peripheral blood Th17 cells in ankylosing spondylitis and rheumatoid arthritis
    • pmid: 19479869
    • H. Shen, J. C. Goodall, J. S. Hill Gaston, Frequency and phenotype of peripheral blood Th17 cells in ankylosing spondylitis and rheumatoid arthritis. Arthritis Rheum. 60, 1647-1656 (2009). doi: 10.1002/art.24568; pmid: 19479869
    • (2009) Arthritis Rheum. , vol.60 , pp. 1647-1656
    • Shen, H.1    Goodall, J.C.2    Hill Gaston, J.S.3
  • 28
    • 80053911955 scopus 로고    scopus 로고
    • Increased frequency of circulating Th22 in addition to Th17 and Th2 lymphocytes in systemic sclerosis: Association with interstitial lung disease
    • pmid: 21996293
    • M. E. Truchetet, N. C. Brembilla, E. Montanari, Y. Allanore, C. Chizzolini, Increased frequency of circulating Th22 in addition to Th17 and Th2 lymphocytes in systemic sclerosis: Association with interstitial lung disease. Arthritis Res. Ther. 13, R166 (2011). doi: 10.1186/ar3486; pmid: 21996293
    • (2011) Arthritis Res. Ther. , vol.13 , pp. R166
    • Truchetet, M.E.1    Brembilla, N.C.2    Montanari, E.3    Allanore, Y.4    Chizzolini, C.5
  • 29
    • 27144467425 scopus 로고    scopus 로고
    • Molecular signatures distinguish human central memory from effector memory CD8 T cell subsets
    • pmid: 16237082
    • T.Willinger, T. Freeman, H. Hasegawa, A. J. McMichael, M. F. Callan, Molecular signatures distinguish human central memory from effector memory CD8 T cell subsets. J. Immunol. 175, 5895-5903 (2005). doi: 10.4049/jimmunol.175.9.5895; pmid: 16237082
    • (2005) J. Immunol. , vol.175 , pp. 5895-5903
    • Willinger, T.1    Freeman, T.2    Hasegawa, H.3    McMichael, A.J.4    Callan, M.F.5
  • 30
    • 26044436515 scopus 로고    scopus 로고
    • Differential expression of human granzymes A, B, and K in natural killer cells and during CD8+ T cell differentiation in peripheral blood
    • pmid: 16106370
    • K. Bratke, M. Kuepper, B. Bade, J. C. Virchow Jr., W. Luttmann, Differential expression of human granzymes A, B, and K in natural killer cells and during CD8+ T cell differentiation in peripheral blood. Eur. J. Immunol. 35, 2608-2616 (2005). doi: 10.1002/eji.200526122; pmid: 16106370
    • (2005) Eur. J. Immunol. , vol.35 , pp. 2608-2616
    • Bratke, K.1    Kuepper, M.2    Bade, B.3    Virchow, J.C.4    Luttmann, W.5
  • 31
    • 84856278095 scopus 로고    scopus 로고
    • Cytometry by time-of-flight shows combinatorial cytokine expression and virus-specific cell niches within a continuum of CD8+ T cell phenotypes
    • pmid: 22265676
    • + T cell phenotypes. Immunity 36, 142- 152 (2012). doi: 10.1016/j.immuni.2012.01.002; pmid: 22265676
    • (2012) Immunity , vol.36 , pp. 142-152
    • Newell, E.W.1    Sigal, N.2    Bendall, S.C.3    Nolan, G.P.4    Davis, M.M.5
  • 32
    • 41549126364 scopus 로고    scopus 로고
    • Precision and linearity targets for validation of an IFNgamma ELISPOT, cytokine flow cytometry, and tetramer assay using CMV peptides
    • pmid: 18366814
    • H. T. Maecker et al., Precision and linearity targets for validation of an IFNgamma ELISPOT, cytokine flow cytometry, and tetramer assay using CMV peptides. BMC Immunol. 9, 9 (2008). doi: 10.1186/1471-2172-9-9; pmid: 18366814
    • (2008) BMC Immunol. , vol.9 , pp. 9
    • Maecker, H.T.1
  • 33
    • 79955750055 scopus 로고    scopus 로고
    • Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum
    • pmid: 21551058
    • S. C. Bendall et al., Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science 332, 687-696 (2011). doi: 10.1126/ science.1198704; pmid: 21551058
    • (2011) Science , vol.332 , pp. 687-696
    • Bendall, S.C.1
  • 34
    • 3543069879 scopus 로고    scopus 로고
    • Seventeen-colour flow cytometry: Unravelling the immune system
    • pmid: 15286731
    • S. P. Perfetto, P. K. Chattopadhyay, M. Roederer, Seventeen-colour flow cytometry: Unravelling the immune system. Nat. Rev. Immunol. 4, 648-655 (2004). doi: 10.1038/nri1416; pmid: 15286731
    • (2004) Nat. Rev. Immunol. , vol.4 , pp. 648-655
    • Perfetto, S.P.1    Chattopadhyay, P.K.2    Roederer, M.3
  • 35
    • 84903993800 scopus 로고    scopus 로고
    • Linking T-cell receptor sequence to functional phenotype at the single-cell level
    • pmid: 24952902
    • A. Han, J. Glanville, L. Hansmann, M. M. Davis, Linking T-cell receptor sequence to functional phenotype at the single-cell level. Nat. Biotechnol. 32, 684-692 (2014). doi: 10.1038/ nbt.2938; pmid: 24952902
    • (2014) Nat. Biotechnol. , vol.32 , pp. 684-692
    • Han, A.1    Glanville, J.2    Hansmann, L.3    Davis, M.M.4
  • 36
    • 84864880991 scopus 로고    scopus 로고
    • Full-length mRNA-Seq from single-cell levels of RNA and individual circulating tumor cells
    • pmid: 22820318
    • D. Ramsköld et al., Full-length mRNA-Seq from single-cell levels of RNA and individual circulating tumor cells. Nat. Biotechnol. 30, 777-782 (2012). doi: 10.1038/nbt.2282; pmid: 22820318
    • (2012) Nat. Biotechnol. , vol.30 , pp. 777-782
    • Ramsköld, D.1
  • 37
    • 84887101406 scopus 로고    scopus 로고
    • Smart-seq2 for sensitive full-length transcriptome profiling in single cells
    • pmid: 24056875
    • S. Picelli et al., Smart-seq2 for sensitive full-length transcriptome profiling in single cells. Nat. Methods 10, 1096-1098 (2013). doi: 10.1038/nmeth.2639; pmid: 24056875
    • (2013) Nat. Methods , vol.10 , pp. 1096-1098
    • Picelli, S.1
  • 38
    • 84876085773 scopus 로고    scopus 로고
    • Quartz-Seq: A highly reproducible and sensitive single-cell RNA sequencing method, reveals nongenetic gene-expression heterogeneity
    • pmid: 23594475
    • Y. Sasagawa et al., Quartz-Seq: A highly reproducible and sensitive single-cell RNA sequencing method, reveals nongenetic gene-expression heterogeneity. Genome Biol. 14, R31 (2013). doi: 10.1186/gb-2013-14-4-r31; pmid: 23594475
    • (2013) Genome Biol. , vol.14 , pp. R31
    • Sasagawa, Y.1
  • 39
    • 84866953427 scopus 로고    scopus 로고
    • CEL-Seq: Single-cell RNA-Seq bymultiplexed linear amplification
    • pmid: 22939981
    • T. Hashimshony, F. Wagner, N. Sher, I. Yanai, CEL-Seq: Single-cell RNA-Seq bymultiplexed linear amplification. Cell Reports 2, 666-673 (2012). doi: 10.1016/j.celrep.2012.08.003;pmid: 22939981
    • (2012) Cell Reports , vol.2 , pp. 666-673
    • Hashimshony, T.1    Wagner, F.2    Sher, N.3    Yanai, I.4
  • 40
    • 41749104588 scopus 로고    scopus 로고
    • Single-molecule DNA sequencing of a viral genome
    • pmid: 18388294
    • T. D. Harris et al., Single-molecule DNA sequencing of a viral genome. Science 320, 106-109 (2008). pmid: 18388294
    • (2008) Science , vol.320 , pp. 106-109
    • Harris, T.D.1
  • 41
    • 58149234737 scopus 로고    scopus 로고
    • Time DNA sequencing from single polymerase molecules
    • pmid: 19023044
    • J. Eid et al., Real-time DNA sequencing from single polymerase molecules. Science 323, 133-138 (2009). doi: 10.1126/ science.1162986; pmid: 19023044
    • (2009) Science , vol.323 , pp. 133-138
    • Eid, J.1
  • 42
    • 64449088698 scopus 로고    scopus 로고
    • Continuous base identification for single-molecule nanopore DNA sequencing
    • pmid: 19350039
    • J. Clarke et al., Continuous base identification for single-molecule nanopore DNA sequencing. Nat. Nanotechnol. 4, 265-270 (2009). doi: 10.1038/nnano.2009.12; pmid: 19350039
    • (2009) Nat. Nanotechnol. , vol.4 , pp. 265-270
    • Clarke, J.1
  • 43
    • 84922321862 scopus 로고    scopus 로고
    • Low-coverage single-cell mRNA sequencing reveals cellular heterogeneity and activated signaling pathways in developing cerebral cortex
    • pmid: 25086649
    • A. A. Pollen et al., Low-coverage single-cell mRNA sequencing reveals cellular heterogeneity and activated signaling pathways in developing cerebral cortex. Nat. Biotechnol. 32, 1053-1058 (2014). doi: 10.1038/nbt.2967; pmid: 25086649
    • (2014) Nat. Biotechnol. , vol.32 , pp. 1053-1058
    • Pollen, A.A.1
  • 44
    • 84922334171 scopus 로고    scopus 로고
    • How deep is enough in single-cell RNAseq?
    • pmid: 25299920
    • A. M. Streets, Y. Huang, How deep is enough in single-cell RNAseq? Nat. Biotechnol. 32, 1005-1006 (2014). doi: 10.1038/ nbt.3039; pmid: 25299920
    • (2014) Nat. Biotechnol. , vol.32 , pp. 1005-1006
    • Streets, A.M.1    Huang, Y.2
  • 45
    • 84859210032 scopus 로고    scopus 로고
    • Fast gapped-read alignment with Bowtie 2
    • pmid: 22388286
    • B. Langmead, S. L. Salzberg, Fast gapped-read alignment with Bowtie 2. Nat. Methods 9, 357-359 (2012). doi: 10.1038/ nmeth.1923; pmid: 22388286
    • (2012) Nat. Methods , vol.9 , pp. 357-359
    • Langmead, B.1    Salzberg, S.L.2
  • 46
    • 40149103278 scopus 로고    scopus 로고
    • Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex
    • pmid: 18264105
    • M. Hamady, J. J. Walker, J. K. Harris, N. J. Gold, R. Knight, Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex. Nat. Methods 5, 235-237 (2008). doi: 10.1038/nmeth.1184; pmid: 18264105
    • (2008) Nat. Methods , vol.5 , pp. 235-237
    • Hamady, M.1    Walker, J.J.2    Harris, J.K.3    Gold, N.J.4    Knight, R.5


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