Active Site Desolvation and Thermostability Trade-Offs in the Evolution of Catalytically Diverse Triazine Hydrolases

Biochemistry

Volumn 55, Issue 45, 2016, Pages 6304-6313

  Sugrue, Elena ^a   Carr, Paul D ^a   Scott, Colin ^b   Jackson, Colin J ^a  

^a AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

^b CSIRO   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CATALYSIS; COMMERCE; CRYSTAL STRUCTURE; ECONOMIC AND SOCIAL EFFECTS; HYDROLASES; HYDROPHOBICITY; STABILITY;

BACTERIAL STRAINS; CHARGED RESIDUES; ENZYME ACTIVE SITES; HIGH RESOLUTION CRYSTAL STRUCTURE; HYDROPHILIC RESIDUES; MICROENVIRONMENTS; NATURAL EVOLUTION; RELATIVE CONTRIBUTION;

ENZYMES;

GLUTAMIC ACID; GLUTAMINE; HYDROLASE; ISOENZYME; TRIAZINE DERIVATIVE; TRIAZINE HYDROLASE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; PROTEIN BINDING;

ARTICLE; BACTERIAL STRAIN; BACTERIUM; CATALYSIS; CONTROLLED STUDY; CRYSTAL STRUCTURE; CRYSTALLIZATION; ENZYME ACTIVE SITE; HYDROPHILICITY; HYDROPHOBICITY; NOCARDIOIDES; NONHUMAN; NUCLEOPHILICITY; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STABILITY; STATIC ELECTRICITY; SUBSTITUTION REACTION; THERMOSTABILITY; ACTINOBACTERIA; BIOCATALYSIS; CHEMICAL PHENOMENA; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; MOLECULAR EVOLUTION; MOLECULAR MODEL; MUTATION; PROTEIN DOMAIN; TEMPERATURE; X RAY CRYSTALLOGRAPHY;

ACTINOBACTERIA; BACTERIAL PROTEINS; BIOCATALYSIS; CATALYTIC DOMAIN; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; ENZYME STABILITY; EVOLUTION, MOLECULAR; GLUTAMIC ACID; HYDROLASES; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; KINETICS; MODELS, MOLECULAR; MOLECULAR STRUCTURE; MUTATION; PROTEIN BINDING; PROTEIN DOMAINS; TEMPERATURE; TRIAZINES;

EID: 84995783980     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/acs.biochem.6b00731     Document Type: Article

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