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Volumn 40, Issue 7, 2001, Pages 1984-1995

The structural basis for the perturbed pKa of the catalytic base in 4-oxalocrotonate tautomerase: Kinetic and structural effects of mutations of Phe-50

(5) Czerwinski, R M b Harris, T K a,c Massiah, M A a Mildvan, A S a Whitman, C P b

a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE (United States)

b UNIVERSITY OF TEXAS AT AUSTIN (United States)

c UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE (United States)

Author keywords

[No Author keywords available]

Indexed keywords

4 OXALOCROTONATE TAUTOMERASE; ENZYME; NITROGEN 15; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; DIELECTRIC CONSTANT; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME STRUCTURE; HYDROPHOBICITY; ISOMERISM; MASS SPECTROMETRY; MICHAELIS CONSTANT; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; NUCLEAR OVERHAUSER EFFECT; PH; PKA; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; ULTRAVIOLET SPECTROSCOPY;

ALANINE; AMINO ACID SEQUENCE; CATALYSIS; CIRCULAR DICHROISM; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; ISOMERASES; KINETICS; MODELS, CHEMICAL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PHENYLALANINE; POLYMERASE CHAIN REACTION; PROLINE; RECOMBINANT PROTEINS; STRUCTURE-ACTIVITY RELATIONSHIP; TITRIMETRY; TYROSINE;

EID: 0035916245 PISSN: 00062960 EISSN: None Source Type: Journal
DOI: 10.1021/bi0024714 Document Type: Article

Times cited : (80)

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