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Volumn 125, Issue 34, 2003, Pages 10228-10237

Apparent NAC effect in chorismate mutase reflects electrostatic transition state stabilization

Author keywords

[No Author keywords available]

Indexed keywords

ELECTROSTATIC TRANSITION;

EID: 0041429611     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja0356481     Document Type: Article
Times cited : (129)

References (84)
  • 40
    • 0042406198 scopus 로고    scopus 로고
    • note
    • The fact that profiles of the form of Figure 2a are unlikely to exist in most enzymes does not present any conceptual or fundamental problem. This is exactly the profile expected from a protein with RSD and without sufficient binding by distant residues. Of course, the fact that proteins with positive binding energies are not found in most cases, provides a major support for the argument against the RSD proposals.
  • 45
    • 0041403848 scopus 로고    scopus 로고
    • note
    • Some readers who would start with ref 43 might be confused by our statement about the description of the NAC effect by Kollman and co-workers. However, one should realize that the analysis of the NAC effect by this group started with the clear analysis of ref 42 where the entropic effect (which they called a cratic effect) was added to the free energy profile of the reaction in water. The same approach was used in all of their explicit calculations and summarized in ref 43. Subsequently, ref 44 used the same diagrams to describe the NAC effect, while still considering the same calculations (see Table 1 of ref 44) that were based on the analysis of entropic effects.
  • 69
    • 0042406196 scopus 로고    scopus 로고
    • note
    • w ≅ -13.3 kcal/mol). Thus, the calculated product binding energy is only about 12 kcal/mol. While this is still an overestimate, it does not indicate a major problem with product inhibition.
  • 83
    • 0042907006 scopus 로고    scopus 로고
    • note
    • The work of Jorgensen and co-workers seems to represent a misunderstanding of the NAC proposal. That is, these workers used very different NAC geometries in the protein and in water. These geometries were chosen more or less as the minima of the corresponding PMFs. Actually, the minimum of the protein PMF is the most logical definition of the NAC geometry in the water reaction (see ref 32). Otherwise the NAC proposal has little to do with the effect of the protein.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.