Efficient introduction of specific homozygous and heterozygous mutations using CRISPR/Cas9

Nature

Volumn 533, Issue 7601, 2016, Pages 125-129

  Paquet, Dominik ^a   Kwart, Dylan ^a   Chen, Antonia ^a   Sproul, Andrew ^b,c   Jacob, Samson ^b   Teo, Shaun ^a   Olsen, Kimberly Moore ^a   Gregg, Andrew ^a   Noggle, Scott ^b   Tessier Lavigne, Marc ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

^b New York Stem Cell Foundation Laboratory   (United States)

^c NewYork Presbyterian Hospital   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMYLOID PRECURSOR PROTEIN; GUIDE RNA; PRESENILIN 1; PRESENILIN;

DISEASE TREATMENT; GENE EXPRESSION; GENETIC ENGINEERING; GENOME; MUTATION; NEUROLOGY; PROTEIN; RNA;

ALLELE; ALZHEIMER DISEASE; ARTICLE; BRAIN CELL; CRISPR CAS SYSTEM; CRISPR CAS9 SYSTEM; DNA END JOINING REPAIR; DNA TEMPLATE; GENE MUTATION; GENOTYPE; HEK293 CELL LINE; HETEROZYGOTE; HOMOZYGOTE; HUMAN; HUMAN CELL; INDUCED PLURIPOTENT STEM CELL; NEXT GENERATION SEQUENCING; PHENOTYPE; POINT MUTATION; PRIORITY JOURNAL; SILENT MUTATION; ADOLESCENT; ANIMAL; DNA CLEAVAGE; DNA REPAIR; DOMINANT GENE; DOUBLE STRANDED DNA BREAK; ENZYME SPECIFICITY; FEMALE; GENETIC ASSOCIATION STUDY; GENETIC ENGINEERING; GENETICS; MALE; METABOLISM; MOUSE; MUTAGENESIS; MUTATION; NUCLEOTIDE SEQUENCE; ONSET AGE; PROCEDURES; SECRETION (PROCESS); SEQUENCE HOMOLOGY;

BACTERIA (MICROORGANISMS);

ADOLESCENT; AGE OF ONSET; ALLELES; ALZHEIMER DISEASE; AMYLOID BETA-PROTEIN PRECURSOR; ANIMALS; BASE SEQUENCE; CRISPR-CAS SYSTEMS; DNA BREAKS, DOUBLE-STRANDED; DNA CLEAVAGE; DNA REPAIR; FEMALE; GENES, DOMINANT; GENETIC ASSOCIATION STUDIES; GENETIC ENGINEERING; HETEROZYGOTE; HOMOZYGOTE; HUMANS; INDUCED PLURIPOTENT STEM CELLS; MALE; MICE; MUTAGENESIS; MUTATION; PRESENILINS; RNA, GUIDE; SEQUENCE HOMOLOGY; SUBSTRATE SPECIFICITY; TEMPLATES, GENETIC;

EID: 84971299957     PISSN: 00280836     EISSN: 14764687     Source Type: Journal    
DOI: 10.1038/nature17664     Document Type: Article

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