A Biophysical Model of CRISPR/Cas9 Activity for Rational Design of Genome Editing and Gene Regulation

PLoS Computational Biology

Volumn 12, Issue 1, 2016, Pages

  Farasat, Iman ^a   Salis, Howard M ^a  

^a The Pennsylvania State University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ENERGY; BINDING SITES; DNA; DNA SEQUENCES; FREE ENERGY; GENE ENCODING; QUANTUM THEORY; SITE SELECTION; STATISTICAL MECHANICS;

BIOPHYSICAL MODEL; ENGINEERING APPLICATIONS; EXPRESSION LEVELS; GENE-REGULATION; GROWTH CONDITIONS; RATIONAL DESIGN; RNA SEQUENCES; SUPERHELICAL DENSITY; TARGET ACTIVITY; TYPE II;

GENE EXPRESSION;

CASPASE 9; CRISPR ASSOCIATED PROTEIN; DNA; GUIDE RNA; PROTEIN BINDING;

ARTICLE; BINDING AFFINITY; BIOLOGICAL MODEL; CELL SIZE; CONTROLLED STUDY; DNA CLEAVAGE; DNA REPLICATION; DNA SEQUENCE; DNA SUPERCOILING; ENTEROBACTERIA PHAGE LAMBDA; ENZYME ACTIVITY; ENZYME SUBSTRATE COMPLEX; GENE CONTROL; GENE DOSAGE; GENE EXPRESSION; GENETIC PROCEDURES; GENOME EDITING; GENOME SIZE; HUMAN; NONHUMAN; NUCLEOTIDE BINDING SITE; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; RNA SEQUENCE; THERMODYNAMICS; VALIDATION PROCESS; CHEMISTRY; COMPUTER SIMULATION; GENE EXPRESSION REGULATION; GENETICS; GENOME; METABOLISM; PHYSIOLOGY;

BACTERIOPHAGE LAMBDA; COMPUTER SIMULATION; CRISPR-ASSOCIATED PROTEINS; DNA; GENE EXPRESSION REGULATION; GENOME; MODELS, GENETIC; PROTEIN BINDING; RNA, GUIDE;

EID: 84956760340     PISSN: 1553734X     EISSN: 15537358     Source Type: Journal    
DOI: 10.1371/journal.pcbi.1004724     Document Type: Article

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