Effective gene targeting in rabbits using RNA-guided Cas9 nucleases

Journal of Molecular Cell Biology

Volumn 6, Issue 1, 2014, Pages 97-99

  Yang, Dongshan ^a   Xu, Jie ^a   Zhu, Tianqing ^a   Fan, Jianglin ^b   Lai, Liangxue ^c   Zhang, Jifeng ^a   Chen, Y Eugene ^a  

^a UNIVERSITY OF MICHIGAN HEALTH SYSTEM   (United States)

^b UNIVERSITY OF YAMANASHI   (Japan)

^c GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CAS9 PROTEIN; ENDONUCLEASE; RNA; UNCLASSIFIED DRUG;

ANIMAL TISSUE; EMBRYO; ENZYME ACTIVITY; GENE MUTATION; GENE TARGETING; KNOCKOUT GENE; LETTER; NONHUMAN; RABBIT; RABBIT EMBRYO;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; DEOXYRIBONUCLEASES; GENE KNOCKOUT TECHNIQUES; GENE TARGETING; GENETIC ENGINEERING; MOLECULAR SEQUENCE DATA; RABBITS; RNA;

EID: 84896861273     PISSN: 16742788     EISSN: 17594685     Source Type: Journal    
DOI: 10.1093/jmcb/mjt047     Document Type: Letter

References (10)

1. Chesne, P., Adenot, P.G., Viglietta, C., et al. (2002). Cloned rabbits produced by nuclear transfer from adult somatic cells. Nat. Biotechnol. 20, 366-369.
2. Conklin, B.R. (2013). Sculpting genomes with a hammer and chisel. Nat. Methods 10, 839-840.
3. Fan, J., and Watanabe, T. (2003). Transgenic rabbits as therapeutic protein bioreactors and human disease models. Pharmacol. Ther. 99, 261-282.
4. Fu, Y., Foden, J.A., Khayter, C., et al. (2013). High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat. Biotechnol. 31, 822-826.
5. Gaj, T., Gersbach, C.A., and Barbas, C.F., III (2013). ZFN, TALEN, and CRISPR/Cas-basedmethods forgenome engineering. Trends Biotechnol. 31, 397-405.
6. Hsu, P.D., Scott, D.A., Weinstein, J.A., et al. (2013). DNA targeting specificity of RNA-guided Cas9 nucleases. Nat. Biotechnol. 31, 827-832.
7. Li, D., Qiu, Z., Shao, Y., et al. (2013a). Heritable gene targeting in the mouse and rat using a CRISPR-Cas system. Nat. Biotechnol. 31, 681-683.
8. Li, W., Teng, F., Li, T., et al. (2013b). Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems. Nat. Biotechnol. 31, 684-686.
9. Wang, H., Yang, H., Shivalila, C.S., et al. (2013). One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell 153, 910-918.
10. Yang, H., Wang, H., Shivalila, C.S., et al. (2013). One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering. Cell 154, 1370-1379.