Conformational Analysis of Large and Highly Disulfide-Stabilized Proteins by Integrating Online Electrochemical Reduction into an Optimized H/D Exchange Mass Spectrometry Workflow

Analytical Chemistry

Volumn 87, Issue 17, 2015, Pages 8880-8888

  Trabjerg, Esben ^a,b   Jakobsen, Rasmus U ^a   Mysling, Simon ^c   Christensen, Søren ^b   Jørgensen, Thomas J D ^d   Rand, Kasper D ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b H LUNDBECK A S   (Denmark)

^c University of Copenhagen   (Denmark)

^d UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; CHEMICAL ANALYSIS; COVALENT BONDS; DRUG PRODUCTS; ELECTROLYTIC REDUCTION; HYDROGEN BONDS; MASS SPECTROMETRY; PHOSPHORUS COMPOUNDS; PROTEINS; SOCIAL NETWORKING (ONLINE); SPECTROMETRY; SULFUR COMPOUNDS;

CONFORMATIONAL ANALYSIS; ELECTROCHEMICAL REDUCTIONS; ENZYMATIC DIGESTIONS; HEAVY AND LIGHT CHAINS; HYDROGEN/DEUTERIUM EXCHANGE; NERVE GROWTH FACTOR; SEQUENCE COVERAGES; TRIS(2-CARBOXYETHYL) PHOSPHINE;

REDUCTION;

DISULFIDE; IMMUNOGLOBULIN G; MONOCLONAL ANTIBODY; NERVE GROWTH FACTOR;

CHEMICAL STRUCTURE; CHEMISTRY; DEUTERIUM HYDROGEN EXCHANGE; ELECTROCHEMICAL ANALYSIS; INTERNET; MASS SPECTROMETRY; OXIDATION REDUCTION REACTION; PH; PROTEIN CONFORMATION; TEMPERATURE;

ANTIBODIES, MONOCLONAL; DEUTERIUM EXCHANGE MEASUREMENT; DISULFIDES; ELECTROCHEMICAL TECHNIQUES; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN G; INTERNET; MASS SPECTROMETRY; MODELS, MOLECULAR; NERVE GROWTH FACTOR; OXIDATION-REDUCTION; PROTEIN CONFORMATION; TEMPERATURE;

EID: 84940985057     PISSN: 00032700     EISSN: 15206882     Source Type: Journal    
DOI: 10.1021/acs.analchem.5b01996     Document Type: Article

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