Simultaneous reduction and digestion of proteins with disulfide bonds for hydrogen/deuterium exchange monitored by mass spectrometry

Analytical Chemistry

Volumn 82, Issue 4, 2010, Pages 1450-1454

  Zhang, Hui Min ^a,b   McLoughlin, Shaun M ^c   Frausto, Stephen D ^d   Tang, Hengli ^d   Emmett, Mark R ^b,d   Marshall, Alan G ^b,d  

^a FLORIDA STATE UNIVERSITY   (United States)

^b Institute of Molecular Biophysics   (United States)

^c ABBOTT LABORATORIES   (United States)

^d Department of Biology ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DISULFIDE BONDS; DISULFIDE LINKAGES; H/D EXCHANGE; H/D EXCHANGE REACTIONS; HIGHER-ORDER STRUCTURE; HYDROGEN/DEUTERIUM EXCHANGE; MASS ANALYSIS; SIMULTANEOUS REDUCTION;

CHEMICAL REACTIONS; MASS SPECTROMETRY; UREA;

PEPTIDES;

DISULFIDE; LIGAND; NS 5 PROTEIN, HEPATITIS C VIRUS; NS-5 PROTEIN, HEPATITIS C VIRUS; PEPSIN A; PROTEIN; REDUCING AGENT; VIRUS PROTEIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; ASPERGILLUS; CHEMICAL STRUCTURE; CHEMISTRY; DEUTERIUM HYDROGEN EXCHANGE; DRUG EFFECT; ENZYME STABILITY; ENZYMOLOGY; MASS SPECTROMETRY; METABOLISM; MOLECULAR GENETICS; OXIDATION REDUCTION REACTION; PROTEIN DENATURATION; PROTEIN TERTIARY STRUCTURE; SWINE; TIME;

AMINO ACID SEQUENCE; ANIMALS; ASPERGILLUS; DEUTERIUM EXCHANGE MEASUREMENT; DISULFIDES; ENZYME STABILITY; LIGANDS; MASS SPECTROMETRY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; OXIDATION-REDUCTION; PEPSIN A; PROTEIN DENATURATION; PROTEIN STRUCTURE, TERTIARY; PROTEINS; REDUCING AGENTS; SWINE; TIME FACTORS; VIRAL NONSTRUCTURAL PROTEINS;

EID: 76849116408     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac902550n     Document Type: Article

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