Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice

Genome Biology

Volumn 16, Issue 1, 2015, Pages

  Aida, Tomomi ^a   Chiyo, Keiho ^a   Usami, Takako ^a   Ishikubo, Harumi ^a   Imahashi, Risa ^a   Wada, Yusaku ^d   Tanaka, Kenji F ^c   Sakuma, Tetsushi ^b   Yamamoto, Takashi ^b   Tanaka, Kohichi ^a,e  

^a TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

^b HIROSHIMA UNIVERSITY   (Japan)

^c KEIO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^d FASMAC Co   (Japan)

^e JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CAS9 PROTEIN; CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN; CRISPR ASSOCIATED PROTEIN; CRRNA; MESSENGER RNA; NUCLEAR PROTEIN; RNA; TRACRRNA; UNCLASSIFIED DRUG; RECOMBINANT PROTEIN;

ACTB GENE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CAS9 GENE; COMPLEX FORMATION; CONTROLLED STUDY; FEMALE; FUNCTIONAL GENOMICS; GENE; GENE CASSETTE; GENE LOCUS; GENE TARGETING; HUMAN; HUMAN CELL; KNOCKOUT MOUSE; MOLECULAR CLONING; MOUSE; NEWBORN; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; RNA TRANSCRIPTION; SEQUENCE ANALYSIS; ANIMAL; CELL LINE; CRISPR CAS SYSTEM; DNA SEQUENCE; GENETICS; HEK293 CELL LINE; MALE; METABOLISM; REPORTER GENE;

MUS;

ANIMALS; CELL LINE; CLONING, MOLECULAR; CRISPR-CAS SYSTEMS; FEMALE; GENE KNOCK-IN TECHNIQUES; GENE TARGETING; GENES, REPORTER; GENETIC LOCI; HEK293 CELLS; HUMANS; MALE; MICE; MICE, KNOCKOUT; RECOMBINANT PROTEINS; RNA, MESSENGER; SEQUENCE ANALYSIS, DNA;

EID: 84938945636     PISSN: 14747596     EISSN: 1474760X     Source Type: Journal    
DOI: 10.1186/s13059-015-0653-x     Document Type: Article

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