Supplemental protein in support of muscle mass and health: Advantage whey

Journal of Food Science

Volumn 80, Issue S1, 2015, Pages A8-A15

  Devries, Michaela C ^a   Phillips, Stuart M ^a  

^a MCMASTER UNIVERSITY   (Canada)

Author keywords

Caloric restriction; Hypertrophy; Muscle; Protein; Sarcopenia; Whey

Indexed keywords

MILK PROTEIN; WHEY PROTEIN;

ANALYSIS; DAIRY PRODUCT; GROWTH, DEVELOPMENT AND AGING; HEALTH; HUMAN; METABOLISM; QUALITY OF LIFE; SKELETAL MUSCLE;

DAIRY PRODUCTS; HEALTH; HUMANS; MILK PROTEINS; MUSCLE, SKELETAL; QUALITY OF LIFE;

EID: 84924778469     PISSN: 00221147     EISSN: 17503841     Source Type: Journal    
DOI: 10.1111/1750-3841.12802     Document Type: Article

References (97)

1. Abargouei A, Janghorbani M, Salehi-Marzijarani M, Esmailzadeh A. 2012. Effect of dairy consumption on weight and body composition in adults: a systematic review and meta-analysis of randomized controlled clinical trials. Int J Obes 36:1485-93.
2. Andersen LL, Tufekovic G, Zebris M, Crameri R, Verlaan G, Kjaer M, Suetta C, Magnusson P, Aagaard P. 2005. The effect of resistance training combined with timed ingestion of protein on muscle fiber size and muscle strength. Metabolism 54:151-6.
3. Anthony J, Yoshizawa F, Anthony T, Vary T, Jefferson L, Kimball S. 2000. Leucine stimulates translation initiation in skeletal muscle of postabsorptive rats via a rapamycin-sensitive pathway. J Nutr 130:2413-9.
4. Anthony J, Anthony T, Kimball S, Jefferson L. 2001. Signalling pathways involved in translational control of protein synthesis in skeletal muscle by leucine. J Nutr 131:856S-60S.
5. Areta J, Burke L, Camera D, West D, Crawshay S, Moore DR, Stellingwerff T, Phillips S, Hawley J. 2014. Reduced resting skeletal muscle protein synthesis is rescued by resistance exercise and protein ingestion following short-term energy deficit. Am J Physiol Endocrinol Metab 306:E989-E97.
6. Baer D, Stote K, Paul D, Harris G, Rumpler W, Clevidence B. 2011. Whey protein but not soy protein supplementation alters body weight and composition in free-living overweight and obese adults. J Nutr 141(8):1489-94.
7. Bauer J, Biolo G, Cederholm T, Cesari M, Cruz-Jentoft A, Morley J, Phillips S, Sieber C, Stehle P, Teta D, Visvanathan R, Volpi E, Boirie Y. 2013. Evidence-based recommendation for optimal dietary protein intake in older people: a position paper from the PROT-AGE Study Group. J Am Med Dir Assoc 14(8):542-59.
8. Berner L, Becker G, Wise M, Doi J. 2013. Characterization of dietary protein among older adults in the United States: amount, animal sources, and meal patters. J Acad Nutr Diet 113:809-15.
9. Biolo G, Tipton K, Klein S, Wolfe RR. 1997. An abundant supply of amino acids enhances the metabolic effect of exercise on muscle protein. Am J Physiol 273(1):E122-E9.
10. Bird S, Tarpenning K, Marino F. 2006. Independent and combined effets of liquid carbohydrate/essential amino acid ingestion on hormonal and muscular adaptations following resistance training in untrained men. Eur J Appl Physiol 97(2):225-38.
11. Boirie Y, Dangin M, Gachon P, Vasson M, Maubois J, Beaufrere B. 1997. Slow and fast dietary proteins differently modulate postprandial protein accretion. Proc Nath Acad Sci USA 94:14930-5.
12. Bos C, Metges CG, C, Petzke K, Pueyo M, Morens C, Everwand J, Benamouzig R. 2003. Postprandial kinetics of dietary amino acids are the main determinant of their metabolism after soy or milk protein ingestion in humans. J Nutr 133:1308-15.
13. Burd N, Yang Y, Moore DR, Tang JE, Tarnopolsky MA, Phillips S. 2012. Greater stimulation of myofibrillar protein synthesis with ingestion of whey protein isolate v. micellar casein at rest and after resistance exercise in elderly men. Br J Nutr 108:958-62.
14. Candow DG, Burke NC, Smith-Palmer T, Burke DG. 2006. Effect of whey and soy protein supplementation combined with resistance training in young adults. Int J Sport Nutr Exerc Metab 16(3):233-44.
15. Cermak N, Res P, de Groot L, Saris H, van Loon L. 2012. Protein supplementation augments the adaptive response of skeletal muscle to resistance-type exercise training: a meta-analysis. Am J Clin Nutr 96:1454-64.
16. Chen M, Pan A, Malik V, Hu F. 2012. Effects of dairy intake on body weight and fat: a meta-analysis of randomized controlled trials. Am J Clin Nutr 96(4):735-47.
17. Churchward-Venne T, Breen L, Di Donato D, Hector A, Mitchell C, Moore DR, Stellingwerff T, Breuille D, Offord E, Baker S, Phillips S. 2014. Leucine supplementation of a low-protein mixed macronutrient beverage enhances myofibrillar protein synthesis in young men: a double-blind, randomized trial. Am J Clin Nutr 99(2):276-86.
18. Claessens M, van Baak M, Monsheimer S, Saris W. 2009. The effect of a low-fat, high-protein or high-carbohydrate ad libitum diet on weight loss maintenance and metabolic risk factors. Int J Obes 33:296-304.
19. Coburn JW, Housh DJ, Housh TJ, Malek MH, Beck TW, Cramer JT, Johnson GO, Donlin PE. 2006. Effects of leucine and whey protein supplementation during eight weeks of unilateral resistance training. J Strength Cond Res 20(2):284-91.
20. Coker R, Miller S, Schutzler S, Deutz N, Wolfe RR. 2012. Whey protein and essential amino acids promote the reduction of adipose tissue and increased muscle protein synthesis during caloric restriction-induced weight loss in elderly, obese individuals. Nutr J 11:105.
21. Cribb P, Williams A, Hayes A, Carey M. 2006. The effect of whey isolate on strength, body composition and plasma glutamine. Int J Sport Nutr Exerc Metab 16:494-509.
22. Crozier S, Kimball S, Emmert S, Anthony J, Jefferson L. 2005. Oral leucine administration stimulates protein synthesis in rat skeletal muscle. J Nutr 135:376-82.
23. Cuthbertson D, Smith K, Babraj J, Leese G, Waddell T, Atherton P, Wackerhage H, Taylor P, Rennie M. 2005. Anabolic signaling deficits underlie amino acid resistance of wasting, aging muscle. FASEB J 19(3):422-4.
24. da Silva Alexandre T, de Oliveira Duarte Y, Ferreira Santos J, Wong R, Lebrao M. 2014. Sarcopenia according to the european working group on sarcopenia in older people (EWGSOP) versus Dynapenia as a risk factor for disability in the elderly. J Nutr Health Aging 18(5):547-53.
25. DeFronzo R, Bonadonna R, Ferrannini E. 1992. Pathogenesis of NIDDM. A balanced overview. Diabetes Care 15(3):318-68.
26. DeNysschen C, Burton H, Horvath P, Leddy J, Browne R. 2009. Resistance training with soy vs whey protein supplements in hyperlipidemic males. J Int Soc Sports Nutr 6:8.
27. Deutz N, Bauer J, Barazzoni R, Biolo G, Boirie Y, Bosy-Westphal A, Caderholm T, Cruz-Jentoft A, Krznaric Z, Nair K, Singer P, Teta D, Tipton K, Calder P. 2014. Protein intake and exercise for optimal muscle function with aging: recommendations for the ESPEN Expert Group. Clin Nutr doi: 10.1016/j.clnu.2014.04.007.
28. Dreyer HC, Drummond MJ, Pennings B, Fujita S, Glynn EL, Chinkes DL, Volpi E, Rasmussen BB. 2008. Leucine-enriched essential amino acids and carbohydrate ingestion following resistance exercise enhances mTOR signaling and protein synthesis in human muscle. Am J Physiol Endocrinol Metab 294:E392-E400.
29. Escobar J, Frank J, Suryawan A, Nguyen H, Kimball S, Jefferson L, Davis T. 2006. Regulation of cardiac and skeletal muscle protein synthesis by individual branched-chain amino acids in neonatal pigs. Am J Physiol Endocrinol Metab 290:E612-E21.
30. Esmarck B, Andersen J, Olsen S, Richter E, Mizuno M, Kjaer M. 2001. Timing of postexercise protein intake is important for muscle hypertrophy with resistance training in elderly humans. J Physiol 535(1):301-11.
31. FAO/WHO Expert Consultation. 1991. Protein quality evaluation report of the Joint FAO/WHO Expert Consultation held in Bethseda, Md., USA, in 1989. FAO Food and Nutrition Paper 51, Rome.
32. Fouillet H, Mariotti F, Gaudichon C, Bos C, Tome D. 2002. Peripheral and splanchnic metabolism of dietary nitrogen are differently affected by the protein source in humans as assessed by compartmental modeling. J Nutr 132(1):125-33.
33. Fouillet H, Juillet B, Gaudichon C, Mariotti F, Tome D, Bos C. 2009. Absorption kinetics are a key factor regulating postprandial protein metabolism in response to qualitative and quantitative variations in protein intake. Am J Physiol Regul Integr Comp Physiol 297(6):R1691-705.
34. Frestedt J, Zenk J, Kuskowski M, Ward L, Bastian E. 2008. A whey-protein supplement increases fat loss and spares lean muscle in obese subjects: a randomized human clinical study. Nutr Metab 5:8.
35. Fulgoni V. 2008. Current protein intake in America: analysis of the Nathional Health and Nutrition Examination Survery, 2003-2004. Am J Clin Nutr 87:1554S-7S.
36. Geirsdottir O, Arnarson A, Ramel A, Jonsson P, Thorsdottir I. 2013. Dietary protein intake is associated with lean body mass in community-dwelling older adults. Nutr Res 33(8):608-12.
37. Gray-Donald K, St-Arnauld-McKenzie D, Gaudreau P, Morais J, Shatenstein B, Payette H. 2014. Protein intake protects against weight loss in healthy community-dwelling older adults. J Nutr 144(3):321-6.
38. Hartman J, Tang JE, Wilkinson S, Tarnopolsky M, Lawrence R, Fullerton A, Phillips S. 2007. Consumption of fat-free fluid milk after resistance exercise promotes greater lean mass accretion than does consumption of soy or carbohydrate in young, novice, male weightlifters. Am J Clin Nutr 86(2):373-81.
39. Hoffman JR, Ratamess NA, Tranchina CP, Rashti SL, Kang J, Faigenbaum AD. 2009. Effect of protein-supplement timing on strength, power, and body-compsition changes in resistance-trained men. Int J Sport Nutr Exerc Metab 19(2):172-85.
40. Houston D, Nicklas B, Ding J, Harris T, Tylavsky F, Newman A, Lee J, Sahyoun N, Visser M, Kritchevsky S, Health ABC Study. 2008. Dietary protein intake is associated with lean mass change in older, community-dwelling adults: the Health, Aging, and Body Composition (Health ABC) Study. Am J Clin Nutr 87(1):150-5.
41. Hulmi J, Koyanen V, Selanne H, Kraemer W, Hakkinen K, Mero A. 2009. Acute and long-term effects of resistance exercise with or without protein ingestion on muscle hypertrophy and gene expression. Amino Acids 37:297-308.
42. Jones K, Eller L, Parnell J, Doyle-Baker P, Edwards A, Reimer R. 2013. Effect of a dairy- and calcium-rich diet on weight loss and appetite during energy restriction in overweight and obese adults: a randomized trial. Eur J Clin Nutr 67(4):371-6.
43. Josse AR, Atkinson SA, Tarnopolsky MA, Phillips SM. 2011. Increased consumption of dairy foods and protein during diet- and exercise-induced weight loss promotes fat mass loss and lean mass gain in overweight and obese premenopausal women. J Nutr 141(9):1626-34.
44. Joy J, Lowery R, Wilson J, Purpura M, De Souza E, Wilson S, Kalman D, Dudeck J, Jager R. 2013. The effects of 8 weeks of whey or rice protein supplementation on body composition and exercise performance. Nutr J 12:86.
45. Jurca R, Lamonte M, Barlow C, Kampert J, Church T, Blair S. 2005. Association of muscular strength with incidence of metabolic syndrome in men. Med Sci Sports Exer 37(11):1849-55.
46. Karagounis L, Hawley J. 2010. Skeletal muscle: increasing the size of the locomotor cell. Int J Biochem Cell Biol 42(9):1376-9.
47. Katsanos C, Kobayashi H, Sheffield-Moore M, Aarsland A, Wolfe RR. 2006. A high proportion of leucine is required for optimal stimulation of the rate of muscle protein synthesis by essential amino acids in the elderly. Am J Physiol Endocrinol Metab 291:E381-E7.
48. Koopman R, Wagenmakers AJ, Manders RJ, Zorenc AH, Senden JM, Gorselink M, Keizer HA, van Loon LJ. 2005. Combined ingestion of protein and free leucine with carbohydrate increases postexercise muscle protein synthesis in vivo in male subjects. Am J Physiol Endocrinol Metab 288:E645-E53.
49. Krieger J, Sitren H, Daniels M, Langkamp-Henken B. 2006. Effects of variation in protein and carbohydrate intake on body mass and composition during energy restriction: a meta-regression. Am J Clin Nutr 83:260-74.
50. Landi F, Liperoti R, Russo A, Giovannini S, Tosato M, Capoluongo E, Bernabei R, Onder G. 2012. Sarcopenia as a risk factor for falls in elderly individuals: results from the il SIRENTE study. Clin Nutr 31(5):652-8.
51. Lorenzen J, Frederiksen R, Hoppe C, Hvid R, Astrup A. 2012. The effect of milk proteins on appetite regulation and diet-induced thermogenesis. Eur J Clin Nutr 66:622-7.
52. Luiking Y, Deutz N, Jakel M, Soeters P. 2005. Casein and soy protein meals differentially affect whole-body and splanchnic protein metabolism in healthy humans. J Nutr 135:1080-7.
53. Lutsey P, Steffen L, Stevens J. 2008. Dietary intake and the development of the metabolic syndrome: the Atherosclerosis Risk in Communities study. Circulation 117:754-61.
54. Miller P, Alexander D, Perez V. 2014. Effects of whey protein and resistance exercise on body composition: a meta-analysis of randomized controlled trials. J Am Coll Nutr 33(2):163-75.
55. Mojtahedi M, Thorpe M, Karampinos D, Johnson C, Layman D, Georgiadis J, Evans E. 2011. The effects of a higher protein intake during energy restriction and changes in body composition and physical function in older women. J Gerontol A Biol Sci Med Sci 66A(11):1218-25.
56. Moore D, Robinson M, Fry J, Tang J, Glover E, Wilkinson S, Prior T, Tarnopolsky M, Phillips S. 2009. Ingested protein dose response of muscle and albumin protein synthesis after resistance exercise in young men. Am J Clin Nutr 89(1):161-8.
57. Moore D, Churchward-Venne T, Witard O, Breen L, Burd N, Tipton K, Phillips S. 2014. Protein ingestion to stimulate myofibrillar protein synthesis requires greater relative protein intakes in healthy older versus younger men. J Gerontol A Biol Sci Med Sci doi:10.1093/gerona/glu103.
58. Morley J. 1997. Anorexia of aging: physiologic and pathologic. Am J Clin Nutr 66(4):760-73.
59. Newby P, Muller D, Hallfrisch J, Qiao N, Andres R, Tucker K. 2003. Dietary patterns and changes in body mass index and waist circumference in adults. Am J Clin Nutr 77(6):1417-25.
60. Paddon-Jones D, Rasmussen B. 2009. Dietary protein recommendations and the prevention of sarcopenia. Curr Opin Clin Nutr Metab Care 12:86-90.
61. Pal S, Ellis V. 2010. The acute effects of four protein meals on insulin, glucose, appetite and energy intake in lean men. Br J Nutr 104:1241-8.
62. Pasiakos SM, Vislocky LM, Carbone JW, Altieri N, Konopelski K, Freake HC, Anderson JM, Ferrando AA, Wolfe RR, Rodriguez NR. 2010. Acute energy deprivation affects skeletal muscle protein synthesis and associated intracellular signaling proteins in physically active adults. J Nutr 140:745-51.
63. Pennings B, Boirie Y, Senden J, Gijsen A, Kuipers H, van Loon L. 2011. Whey protein stimulates postprandial muscle protein accretion more effectively than do casein and casein hydrolysate in older men. Am J Clin Nutr 93(5):997-1005.
64. Pennings B, Groen B, de Lange A, Gijsen A, Zorenc A, Senden J, van Loon L. 2012. Amino acid absorption and subsequent muscle protein accretion following graded intakes of whey protein in elderly men. Am J Physiol Endocrinol Metab 302(8):E992-E9.
65. Pereira M, Jacobs DJ, Van Horn L, Slattery M, Kartashov A, Ludwig D. 2002. Dairy consumption, obesity, and the insulin resistance syndrome in young adults: the CARDIA study. JAMA 287(16):2081-9.
66. Phillips S. 2009. Physiologic and molecular bases of muscle hypertrophy and atrophy: impact of resistance exercise on human skeletal muscle (protein and exercise dose effects). Appl Physiol Nutr Metab 34:403-10.
67. Phillips S. 2014. A brief review of critical processes in exercise-induced muscular hypertrophy. Sports Med 44(1):71-7.
68. Phillips S, Tipton K, Aarsland A, Wolf S, Wolfe RR. 1997. Mixed muscle protein synthesis and breakdwon after resistance exercise in humans. Am J Physiol 273(1):E99-E107.
69. Ruiz J, Sui X, Lobelo F, Morrow JJ, Jackson A, Sjostrom M, Blair S. 2008. Association between muscular strength and mortality in men: prospective cohort study. BMJ 337:a439.
70. Schoenfeld B, Aragon A, Krieger J. 2013. The effect of protein timing on muscle strength and hypertrophy: a meta-analysis. J Int Soc Sports Nutr 10:53.
71. Scott D, Hayes A, Sanders K, Aitken D, Ebeling P, Jones G. 2014. Operational definitions of sarcopenia and their associations with 5-year changes in falls risk in community-dwelling middle-aged and older adults. Osteoporosis Int 25(1):187-93.
72. Skov A, Toubro S, Ronn B, Holm L, Astrup A. 1999. Randomized trial on protein vs carbohydrate in ad libitum fat reduced diet for the treatment of obesity. Int J Obes Relat Metab Disord 23(5):528-36.
73. Stark M, Lukaszuk J, Prawitz A, Salacinski A. 2012. Protein timing and its effects on muscular hypertophy and strength in individuals engaged in weight-training. J Int Soc Sports Nutr 9:54.
74. Stookey J, Adair L, Popkin B. 2005. Do protein and energy intakes explain long-term changes in body composition? J Nutr Health Aging 9(1):5-17.
75. Tang JE, Phillips S. 2009. Maximizing muscle protein anabolism: the role of protein quality. Curr Opin Clin Nutr Metab Care 12(1):66-71.
76. Tang JE, Moore DR, Kujbida G, Tarnopolsky M, Phillips S. 2009. Ingestion of whey hydrolysate, casein, or soy protein isolate: effects on mixed muscle protein synthesis at rest and following resistance exercise in young men. J Appl Physiol 107(3):987-92.
77. Tieland M, Dirks M, van der Zwaluw N, Verdijk L, van de Rest O, de Groot L, van Loon L. 2012a. Protein supplementation increases muscle mass gain during prolonged resistance-type exercise training in frail elderly people: a randomized, double-blind, placebo-controlled trial. J Am Med Dir Assoc 13(8):713-9.
78. Tieland M, van de Rest O, Dirks M, van der Zwaluw N, Mensink M, van Loon L, de Groot L. 2012b. Protein supplementation improves physical performance in frail elderly people: a randomized, double-blind, placebo-controlled trial. J Am Med Dir Assoc 13:720-6.
79. Tipton K, Elliot T, Cree M, Wolf S, Sanford A, Wolfe RR. 2004. Ingestion of casein and whey proteins result in muscle anabolism after resistance exercise. Med Sci Sports Exer 36(12):2073-81.
80. Tipton K, Elliot T, Cree M, Aarsland A, Sanford A, Wolfe RR. 2007. Stimulation of net muscle protein synthesis by whey protein ingestion before and after exercise. Am J Physiol Endocrinol Metab 292(1):E71-E6.
81. United States Department of Agriculture. The USDA National Nutrient Database for Standard Reference. Nutrient Data Laboratory: Beltsville Human Nutrition Research Centre. http://ndb.nal.usda.gov/. Lastmodified Dec 7, 2011.
82. Velazquez Alva Mdel C, Irigoyen Camacho M, Delgadillo Velazquez J, Lazarevich I. 2013. The relationship between sarcopenia, undernutrition, physical mobility and basic activities of daily living in a group of elderly women of Mexico City. Nutr Hosp 28(2):514-21.
83. Verdijk L, Jonkers R, Gleeson B, Beelen M, Meijer K, Savelberg H, Wodzig W, Dendale P, van Loon L. 2009. Protein supplementation before and after exercise does not further augment skeletal muscle hypertrophy after resistance training in elderly men. Am J Clin Nutr 89(2):608-16.
84. Volek J. 2004. Influence of nutrition on responses to resistance training. Med Sci Sports Exer 36:689-96.
85. Volek J, Volk B, Gomez A, Kunces L, Jupchak B, Freidenreich D, Aristizabal J, Saenz C, Dunn-Lewis C, Ballard K, Quann E, Kawiecki D, Flanagan S, Comstock B, Fragala M, Earp J, Fernandez M, Bruno R, Ptolemy A, Kellogg M, Maresh C, Kraemer W. 2013. Whey protein supplementation during resistance training augments lean body mass. J Am Coll Nutr 32(2):122-35.
86. Volpi E, Mittendorpher B, Rasmussen B, Wolfe RR. 2000. The response of muscle protein anabolism to combined hyperaminoacidemia and glucose-induced hyperinsulinemia is impaired in the elderly. J Clin Endocrinol Metab 85:4481-90.
87. Volpi E, Campbell WW, Dwyer JT, Johnson MA, Jensen GL, Morley JE, Wolfe RR. 2013. Is the optimal level of protein intake for older adults greater than the recommended dietary allowance? J Gerontol A Biol Sci Med Sci 68(6):677-81.
88. West D, Burd N, Coffey V, Baker S, Burke L, Hawley J, Moore DR, Stellingwerff T, Phillips S. 2011. Rapid aminoacidemia enhances myofibrillar protein synthesis and anabolic intramuscular signaling repsonses after resistance exercise. Am J Clin Nutr 94(3):795-803.
89. Westerterp-Plantenga M, Nieuwenhuizen A, Tome D, Soenen S, Westerterp K. 2009. Dietary protein, weight loss, and weight maintenance. Annu Rev Nut 29:21-41.
90. Wilkinson S, Tarnopolsky M, MacDonald M, Macdonald J, Armstrong D, Phillips S. 2007. Consumption of fluid skim milk promotes greater muscle protein accretion after resistance exercise than does consumption of an isonitrogenous and isoenergetic soy-protein beverage. Am J Clin Nutr 85(4):1031-40.
91. Witard O, Jackman S, Breen L, Smith K, Selby A, Tipton K. 2014. Myofibrillar muscle protein synthesis rates subsequent to a meal in response to increasing doses of whey protein at rest and after resistance exercise. Am J Clin Nutr 99(1):86-95.
92. Wolfe RR. 2006. The underappreciated role of muscle in health and disease. Am J Clin Nutr 84:475-82.
93. Wolfe RR, Miller S, Miller K. 2008. Optimal protein intake in the elderly. Clin Nutr 27(5):675-84.
94. Wycherley T, Noakes M, Clifton P, Cleanthous X, Keogh J, Brinkworth G. 2010. Timing of protein ingestion relative to resistance exercise training does not influence body composition, energy expenditure, glycaemic control or cardiometabolic risk factors in a hypocaloric, high protein diet in patients with type 2 diabetes. Diab Obes Metab 12(12):1097-105.
95. Wycherley T, Moran L, Clifton P, Noakes M, Brinkworth G. 2012. Effects of energy-restricted high-protein, low-fat compared with standard-protein, low fat diets: a meta-analysis of randomized controlled trials. Am J Clin Nutr 96(6):1281-98.
96. Yang Y, Breen L, Burd N, Hector A, Churchward-Venne TJ, AR, Tarnopolsky M, Phillips S. 2012a. Resistance exercise enhances myofibrillar protein synthesis with graded intakes of whey protein in older men. Br J Nutr 108(10):1780-8.
97. Yang Y, Churchward-Venne T, Burd N, Breen L, Tarnopolsky MA, Phillips S. 2012b. Myofibrillar protein synthesis following ingestion of soy protein isolate at rest and after resistance exercise in elderly men. Nutr Metab (Lond) 9:57.