Investigation of the correlation between charge and glycosylation of IgG1 variants by liquid chromatography-mass spectrometry

Analytical Biochemistry

Volumn 448, Issue 1, 2014, Pages 82-91

  Yang, Jia Ming ^a   Ai, Junwen ^a   Bao, Yuemei ^a   Yuan, Zhijun ^a   Qin, Yumin ^a   Xie, Yi Wu ^a   Tao, Desheng ^a   Fu, Daotian ^a   Peng, Yucai ^a  

^a Livzon MabPharm ^*  (China)

Author keywords

Charge heterogeneity; Glycosylation; Monoclonal antibody; UPLC MS

Indexed keywords

AMINO ACIDS; CYTOTOXICITY; GLYCOSYLATION; MASS SPECTROMETRY; MONOCLONAL ANTIBODIES;

CATION EXCHANGE CHROMATOGRAPHY; CHARGE HETEROGENEITY; COMPLEMENT-DEPENDENT CYTOTOXICITIES; LIQUID CHROMATOGRAPHY - MASS SPECTROMETRIES; MONOCLONAL ANTIBODIES (MAB); SIMULTANEOUS DETERMINATIONS; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRIES; UPLC-MS;

LIQUID CHROMATOGRAPHY;

EXOPEPTIDASE; GLYCAN; IMMUNOGLOBULIN G1; LYSINE; PYROGLUTAMIC ACID;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; BIOLOGICAL ACTIVITY; CARBOXY TERMINAL SEQUENCE; COMPLEMENT DEPENDENT CYTOTOXICITY; CYCLIZATION; DEAMINATION; FEMALE; GEL PERMEATION CHROMATOGRAPHY; GLYCOSYLATION; MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; PEPTIDE MAPPING; PRIORITY JOURNAL; SEQUENCE ANALYSIS; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY;

CHARGE HETEROGENEITY; GLYCOSYLATION; MONOCLONAL ANTIBODY; UPLC-MS;

ANIMALS; CARBOHYDRATE SEQUENCE; CHO CELLS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, ION EXCHANGE; CRICETINAE; CRICETULUS; GLYCOSYLATION; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN G; LYSINE; MOLECULAR SEQUENCE DATA; PEPTIDE MAPPING; POLYMORPHISM, GENETIC; PROTEIN STRUCTURE, TERTIARY; PYRROLIDONECARBOXYLIC ACID; RECOMBINANT PROTEINS; TANDEM MASS SPECTROMETRY;

EID: 84891758097     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.11.020     Document Type: Article

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